Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0003 and Shewana3_0004 are separated by 177 nucleotides Shewana3_0004 and trmE are separated by 103 nucleotides
Shewana3_0003: Shewana3_0003 - flavodoxin (RefSeq), at 3,107 to 3,547
_0003
Shewana3_0004: Shewana3_0004 - TGF-beta receptor, type I/II extracellular region (RefSeq), at 3,725 to 5,272
_0004
Shewana3_0005: trmE - tRNA modification GTPase TrmE (RefSeq), at 5,376 to 6,737
trmE
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3.989 kb on - strand, within Shewana3_0004 at 4.049 kb on + strand, within Shewana3_0004 at 4.049 kb on + strand, within Shewana3_0004 at 4.057 kb on - strand, within Shewana3_0004 at 4.288 kb on - strand, within Shewana3_0004 at 4.438 kb on - strand, within Shewana3_0004 at 4.479 kb on - strand, within Shewana3_0004 at 4.492 kb on - strand, within Shewana3_0004 at 4.512 kb on + strand, within Shewana3_0004 at 4.578 kb on - strand, within Shewana3_0004 at 4.619 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.786 kb on + strand, within Shewana3_0004 at 4.816 kb on + strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.957 kb on - strand, within Shewana3_0004 at 5.160 kb on + strand at 5.231 kb on + strand at 5.433 kb on - strand at 5.536 kb on - strand, within trmE at 5.557 kb on + strand, within trmE at 5.610 kb on - strand, within trmE at 6.126 kb on + strand, within trmE at 6.126 kb on + strand, within trmE at 6.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,989 - Shewana3_0004 0.17 -0.2 4,049 + Shewana3_0004 0.21 -0.7 4,049 + Shewana3_0004 0.21 +0.3 4,057 - Shewana3_0004 0.21 -1.1 4,288 - Shewana3_0004 0.36 -0.4 4,438 - Shewana3_0004 0.46 -0.6 4,479 - Shewana3_0004 0.49 -0.6 4,492 - Shewana3_0004 0.50 -0.5 4,512 + Shewana3_0004 0.51 +0.8 4,578 - Shewana3_0004 0.55 -1.6 4,619 - Shewana3_0004 0.58 +0.0 4,719 - Shewana3_0004 0.64 -1.2 4,719 - Shewana3_0004 0.64 -0.0 4,786 + Shewana3_0004 0.69 +0.4 4,816 + Shewana3_0004 0.70 +0.6 4,851 - Shewana3_0004 0.73 -0.8 4,851 - Shewana3_0004 0.73 -3.4 4,957 - Shewana3_0004 0.80 -0.4 5,160 + +0.3 5,231 + +0.4 5,433 - +0.7 5,536 - trmE Shewana3_0005 0.12 +1.6 5,557 + trmE Shewana3_0005 0.13 -0.1 5,610 - trmE Shewana3_0005 0.17 +2.0 6,126 + trmE Shewana3_0005 0.55 -1.0 6,126 + trmE Shewana3_0005 0.55 -0.4 6,129 - -0.4
Or see this region's nucleotide sequence