Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1924 and Shew_1925 are separated by 183 nucleotides Shew_1925 and Shew_1926 are separated by 3 nucleotides Shew_1926 and Shew_1927 are separated by 11 nucleotides
Shew_1924: Shew_1924 - succinylglutamate desuccinylase (RefSeq), at 2,221,280 to 2,222,347
_1924
Shew_1925: Shew_1925 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (RefSeq), at 2,222,531 to 2,223,709
_1925
Shew_1926: Shew_1926 - transketolase, central region (RefSeq), at 2,223,713 to 2,224,690
_1926
Shew_1927: Shew_1927 - dihydrolipoamide acetyltransferase (RefSeq), at 2,224,702 to 2,226,264
_1927
Position (kb)
2222
2223
2224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2222.301 kb on + strand at 2222.404 kb on + strand at 2222.869 kb on - strand, within Shew_1925 at 2222.883 kb on + strand, within Shew_1925 at 2223.060 kb on - strand, within Shew_1925 at 2223.096 kb on + strand, within Shew_1925 at 2223.119 kb on + strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.334 kb on + strand, within Shew_1925 at 2223.363 kb on + strand, within Shew_1925 at 2223.831 kb on - strand, within Shew_1926 at 2223.842 kb on - strand, within Shew_1926 at 2224.075 kb on - strand, within Shew_1926
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 2,222,301 + +1.2 2,222,404 + +0.4 2,222,869 - Shew_1925 0.29 -1.3 2,222,883 + Shew_1925 0.30 -2.0 2,223,060 - Shew_1925 0.45 -2.3 2,223,096 + Shew_1925 0.48 +1.9 2,223,119 + Shew_1925 0.50 -1.2 2,223,127 - Shew_1925 0.51 -1.6 2,223,127 - Shew_1925 0.51 -2.9 2,223,334 + Shew_1925 0.68 -2.0 2,223,363 + Shew_1925 0.71 -1.4 2,223,831 - Shew_1926 0.12 -2.3 2,223,842 - Shew_1926 0.13 -0.1 2,224,075 - Shew_1926 0.37 -1.6
Or see this region's nucleotide sequence