Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1754 and Shew_1755 are separated by 25 nucleotides Shew_1755 and Shew_1756 overlap by 1 nucleotides Shew_1756 and Shew_1757 are separated by 2 nucleotides
Shew_1754: Shew_1754 - hypothetical protein (RefSeq), at 2,006,401 to 2,006,565
_1754
Shew_1755: Shew_1755 - hydrogenase expression/synthesis, HypA (RefSeq), at 2,006,591 to 2,006,941
_1755
Shew_1756: Shew_1756 - hydrogenase expression/formation protein HypE (RefSeq), at 2,006,941 to 2,007,951
_1756
Shew_1757: Shew_1757 - hydrogenase expression/formation protein HypD (RefSeq), at 2,007,954 to 2,009,069
_1757
Position (kb)
2006
2007
2008 Strain fitness (log2 ratio)
-2
-1
0
1 at 2005.983 kb on - strand at 2005.983 kb on - strand at 2005.994 kb on - strand at 2006.040 kb on + strand at 2006.063 kb on - strand at 2006.063 kb on - strand at 2006.064 kb on - strand at 2006.260 kb on + strand at 2006.298 kb on + strand at 2006.364 kb on + strand at 2006.364 kb on + strand at 2006.387 kb on + strand at 2006.490 kb on - strand, within Shew_1754 at 2006.556 kb on - strand at 2006.968 kb on - strand at 2007.120 kb on + strand, within Shew_1756 at 2007.195 kb on - strand, within Shew_1756 at 2007.969 kb on - strand at 2008.316 kb on - strand, within Shew_1757 at 2008.346 kb on + strand, within Shew_1757
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 2,005,983 - +0.7 2,005,983 - +0.3 2,005,994 - +0.5 2,006,040 + +0.3 2,006,063 - -0.2 2,006,063 - -0.1 2,006,064 - +0.5 2,006,260 + -0.5 2,006,298 + -2.7 2,006,364 + +0.8 2,006,364 + -0.8 2,006,387 + +0.8 2,006,490 - Shew_1754 0.54 -0.4 2,006,556 - +0.7 2,006,968 - -0.3 2,007,120 + Shew_1756 0.18 -0.7 2,007,195 - Shew_1756 0.25 -0.3 2,007,969 - +0.3 2,008,316 - Shew_1757 0.32 -0.3 2,008,346 + Shew_1757 0.35 -1.9
Or see this region's nucleotide sequence