Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1371 and fliL are separated by 99 nucleotides fliL and fliM are separated by 42 nucleotides fliM and fliN are separated by 25 nucleotides fliN and Shew_1375 are separated by 9 nucleotides Shew_1375 and fliP overlap by 4 nucleotides
Shew_1371: Shew_1371 - flagellar hook-length control protein (RefSeq), at 1,580,960 to 1,582,666
_1371
Shew_1372: fliL - flagellar basal body-associated protein FliL (RefSeq), at 1,582,766 to 1,583,287
fliL
Shew_1373: fliM - flagellar motor switch protein FliM (RefSeq), at 1,583,330 to 1,584,355
fliM
Shew_1374: fliN - flagellar motor switch protein (RefSeq), at 1,584,381 to 1,584,764
fliN
Shew_1375: Shew_1375 - flagellar biosynthesis protein, FliO (RefSeq), at 1,584,774 to 1,585,151
_1375
Shew_1376: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,585,148 to 1,585,915
fliP
Position (kb)
1583
1584
1585 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1583.140 kb on - strand, within fliL at 1583.258 kb on + strand at 1583.368 kb on + strand at 1583.376 kb on - strand at 1583.828 kb on + strand, within fliM at 1584.333 kb on - strand at 1584.397 kb on - strand at 1584.547 kb on + strand, within fliN at 1584.547 kb on + strand, within fliN at 1584.604 kb on + strand, within fliN at 1584.854 kb on - strand, within Shew_1375 at 1584.959 kb on + strand, within Shew_1375
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,583,140 - fliL Shew_1372 0.72 -1.3 1,583,258 + +0.6 1,583,368 + -1.7 1,583,376 - -0.8 1,583,828 + fliM Shew_1373 0.49 -1.1 1,584,333 - -0.6 1,584,397 - -1.2 1,584,547 + fliN Shew_1374 0.43 -1.7 1,584,547 + fliN Shew_1374 0.43 -0.6 1,584,604 + fliN Shew_1374 0.58 -4.3 1,584,854 - Shew_1375 0.21 -2.0 1,584,959 + Shew_1375 0.49 -0.7
Or see this region's nucleotide sequence