Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaA and Shew_1299 are separated by 146 nucleotides Shew_1299 and Shew_1300 are separated by 233 nucleotides Shew_1300 and Shew_1301 are separated by 325 nucleotides
Shew_1298: guaA - bifunctional GMP synthase/glutamine amidotransferase protein (RefSeq), at 1,500,113 to 1,501,690
guaA
Shew_1299: Shew_1299 - CMP/dCMP deaminase, zinc-binding (RefSeq), at 1,501,837 to 1,502,373
_1299
Shew_1300: Shew_1300 - lytic transglycosylase, catalytic (RefSeq), at 1,502,607 to 1,504,037
_1300
Shew_1301: Shew_1301 - phosphoribosylformylglycinamidine synthase (RefSeq), at 1,504,363 to 1,508,244
_1301
Position (kb)
1502
1503
1504
1505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1501.791 kb on + strand at 1502.544 kb on - strand at 1502.566 kb on + strand at 1502.566 kb on + strand at 1502.676 kb on - strand at 1502.866 kb on - strand, within Shew_1300 at 1503.343 kb on + strand, within Shew_1300 at 1503.348 kb on - strand, within Shew_1300 at 1503.404 kb on + strand, within Shew_1300 at 1503.711 kb on - strand, within Shew_1300 at 1503.758 kb on - strand, within Shew_1300 at 1503.895 kb on + strand at 1503.925 kb on - strand at 1504.265 kb on + strand at 1504.427 kb on + strand at 1504.546 kb on - strand at 1504.578 kb on + strand at 1504.836 kb on - strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,501,791 + -0.3 1,502,544 - +1.2 1,502,566 + -0.5 1,502,566 + +0.8 1,502,676 - -0.5 1,502,866 - Shew_1300 0.18 -2.9 1,503,343 + Shew_1300 0.51 +2.9 1,503,348 - Shew_1300 0.52 +0.4 1,503,404 + Shew_1300 0.56 +0.6 1,503,711 - Shew_1300 0.77 +1.2 1,503,758 - Shew_1300 0.80 +1.0 1,503,895 + +0.4 1,503,925 - +1.7 1,504,265 + +0.1 1,504,427 + -0.3 1,504,546 - +0.1 1,504,578 + +3.8 1,504,836 - Shew_1301 0.12 +0.7 1,504,883 + Shew_1301 0.13 -0.1 1,504,883 + Shew_1301 0.13 -0.3
Or see this region's nucleotide sequence