Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1265 and Shew_1266 are separated by 4 nucleotides Shew_1266 and Shew_1267 are separated by 538 nucleotides Shew_1267 and ssrA are separated by 201 nucleotides ssrA and smpB are separated by 122 nucleotides
Shew_1265: Shew_1265 - hypothetical protein (RefSeq), at 1,464,090 to 1,464,632
_1265
Shew_1266: Shew_1266 - XRE family transcriptional regulator (RefSeq), at 1,464,637 to 1,465,020
_1266
Shew_1267: Shew_1267 - phage integrase family protein (RefSeq), at 1,465,559 to 1,466,788
_1267
Shew_R0032: ssrA - no description, at 1,466,990 to 1,467,345
ssrA
Shew_1268: smpB - SsrA-binding protein (RefSeq), at 1,467,468 to 1,467,959
smpB
Position (kb)
1465
1466
1467 Strain fitness (log2 ratio)
-2
-1
0
1 at 1464.825 kb on + strand, within Shew_1266 at 1465.755 kb on + strand, within Shew_1267 at 1465.763 kb on - strand, within Shew_1267 at 1465.989 kb on - strand, within Shew_1267 at 1466.085 kb on - strand, within Shew_1267 at 1466.143 kb on + strand, within Shew_1267 at 1466.280 kb on - strand, within Shew_1267
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,464,825 + Shew_1266 0.49 -1.9 1,465,755 + Shew_1267 0.16 -0.2 1,465,763 - Shew_1267 0.17 +0.6 1,465,989 - Shew_1267 0.35 +0.4 1,466,085 - Shew_1267 0.43 -0.1 1,466,143 + Shew_1267 0.47 +0.1 1,466,280 - Shew_1267 0.59 +0.3
Or see this region's nucleotide sequence