Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1221 and Shew_1222 are separated by 363 nucleotides Shew_1222 and Shew_1223 are separated by 29 nucleotides Shew_1223 and Shew_1224 are separated by 132 nucleotides Shew_1224 and Shew_1225 are separated by 277 nucleotides Shew_1225 and Shew_1226 are separated by 85 nucleotides
Shew_1221: Shew_1221 - sodium ion-translocating decarboxylase, beta subunit (RefSeq), at 1,419,368 to 1,420,501
_1221
Shew_1222: Shew_1222 - hypothetical protein (RefSeq), at 1,420,865 to 1,421,050
_1222
Shew_1223: Shew_1223 - hypothetical protein (RefSeq), at 1,421,080 to 1,421,265
_1223
Shew_1224: Shew_1224 - sodium:dicarboxylate symporter (RefSeq), at 1,421,398 to 1,422,648
_1224
Shew_1225: Shew_1225 - hypothetical protein (RefSeq), at 1,422,926 to 1,423,348
_1225
Shew_1226: Shew_1226 - peptidase M16 domain-containing protein (RefSeq), at 1,423,434 to 1,426,268
_1226
Position (kb)
1421
1422
1423 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1420.573 kb on + strand at 1420.581 kb on - strand at 1420.690 kb on - strand at 1420.975 kb on - strand, within Shew_1222 at 1421.139 kb on + strand, within Shew_1223 at 1421.575 kb on + strand, within Shew_1224 at 1421.705 kb on + strand, within Shew_1224 at 1421.713 kb on - strand, within Shew_1224 at 1422.135 kb on + strand, within Shew_1224 at 1422.312 kb on + strand, within Shew_1224 at 1422.312 kb on + strand, within Shew_1224 at 1422.320 kb on - strand, within Shew_1224 at 1422.720 kb on + strand at 1423.070 kb on + strand, within Shew_1225 at 1423.089 kb on + strand, within Shew_1225 at 1423.251 kb on + strand, within Shew_1225 at 1423.400 kb on + strand at 1423.400 kb on + strand at 1423.408 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,420,573 + -0.4 1,420,581 - -0.5 1,420,690 - -2.3 1,420,975 - Shew_1222 0.59 +0.1 1,421,139 + Shew_1223 0.32 +1.5 1,421,575 + Shew_1224 0.14 +3.0 1,421,705 + Shew_1224 0.25 +3.4 1,421,713 - Shew_1224 0.25 +3.3 1,422,135 + Shew_1224 0.59 +2.9 1,422,312 + Shew_1224 0.73 +3.1 1,422,312 + Shew_1224 0.73 +3.1 1,422,320 - Shew_1224 0.74 +3.3 1,422,720 + +2.9 1,423,070 + Shew_1225 0.34 +0.3 1,423,089 + Shew_1225 0.39 -0.5 1,423,251 + Shew_1225 0.77 +1.2 1,423,400 + -0.1 1,423,400 + +0.2 1,423,408 - -0.1
Or see this region's nucleotide sequence