Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6018 and Pf6N2E2_6019 are separated by 21 nucleotides Pf6N2E2_6019 and Pf6N2E2_6020 are separated by 194 nucleotides Pf6N2E2_6020 and Pf6N2E2_6021 are separated by 144 nucleotides Pf6N2E2_6021 and Pf6N2E2_6022 overlap by 11 nucleotides Pf6N2E2_6022 and Pf6N2E2_6023 are separated by 4 nucleotides
Pf6N2E2_6018 - Fumarylacetoacetate hydrolase family protein, at 6,838,446 to 6,839,144
_6018
Pf6N2E2_6019 - putative oxidoreductase, at 6,839,166 to 6,839,924
_6019
Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075
_6020
Pf6N2E2_6021 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 6,841,220 to 6,841,507
_6021
Pf6N2E2_6022 - StbE replicon stabilization toxin, at 6,841,497 to 6,841,808
_6022
Pf6N2E2_6023 - Putative cytoplasmic protein, at 6,841,813 to 6,842,451
_6023
Position (kb)
6840
6841
6842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6839.139 kb on + strand at 6839.139 kb on + strand at 6839.253 kb on + strand, within Pf6N2E2_6019 at 6839.353 kb on + strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.403 kb on + strand, within Pf6N2E2_6019 at 6839.404 kb on - strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.771 kb on - strand, within Pf6N2E2_6019 at 6839.842 kb on - strand, within Pf6N2E2_6019 at 6840.107 kb on + strand at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.450 kb on + strand, within Pf6N2E2_6020 at 6840.451 kb on - strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.807 kb on - strand, within Pf6N2E2_6020 at 6841.033 kb on + strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.136 kb on - strand at 6841.198 kb on + strand at 6841.198 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.692 kb on + strand, within Pf6N2E2_6022 at 6841.787 kb on + strand at 6841.788 kb on - strand at 6841.865 kb on + strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.928 kb on - strand, within Pf6N2E2_6023
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,839,139 + -0.2 6,839,139 + -1.3 6,839,253 + Pf6N2E2_6019 0.11 +0.6 6,839,353 + Pf6N2E2_6019 0.25 +0.7 6,839,354 - Pf6N2E2_6019 0.25 -1.6 6,839,354 - Pf6N2E2_6019 0.25 +2.8 6,839,354 - Pf6N2E2_6019 0.25 -0.1 6,839,403 + Pf6N2E2_6019 0.31 -0.1 6,839,404 - Pf6N2E2_6019 0.31 -0.5 6,839,697 + Pf6N2E2_6019 0.70 -0.4 6,839,697 + Pf6N2E2_6019 0.70 -0.4 6,839,697 + Pf6N2E2_6019 0.70 -0.3 6,839,698 - Pf6N2E2_6019 0.70 -2.6 6,839,698 - Pf6N2E2_6019 0.70 +1.6 6,839,698 - Pf6N2E2_6019 0.70 -3.0 6,839,698 - Pf6N2E2_6019 0.70 -0.1 6,839,770 + Pf6N2E2_6019 0.80 -0.2 6,839,770 + Pf6N2E2_6019 0.80 +0.1 6,839,771 - Pf6N2E2_6019 0.80 +0.2 6,839,842 - Pf6N2E2_6019 0.89 -1.4 6,840,107 + +0.6 6,840,230 + Pf6N2E2_6020 0.12 -0.0 6,840,230 + Pf6N2E2_6020 0.12 -0.9 6,840,230 + Pf6N2E2_6020 0.12 +0.4 6,840,231 - Pf6N2E2_6020 0.12 -0.3 6,840,231 - Pf6N2E2_6020 0.12 +0.7 6,840,231 - Pf6N2E2_6020 0.12 +0.0 6,840,231 - Pf6N2E2_6020 0.12 -0.1 6,840,231 - Pf6N2E2_6020 0.12 +0.4 6,840,450 + Pf6N2E2_6020 0.35 -0.3 6,840,451 - Pf6N2E2_6020 0.35 +0.1 6,840,647 + Pf6N2E2_6020 0.55 +0.3 6,840,647 + Pf6N2E2_6020 0.55 +0.8 6,840,647 + Pf6N2E2_6020 0.55 -2.0 6,840,647 + Pf6N2E2_6020 0.55 -0.7 6,840,647 + Pf6N2E2_6020 0.55 -0.8 6,840,807 - Pf6N2E2_6020 0.72 +0.3 6,841,033 + +1.3 6,841,034 - +0.6 6,841,034 - +1.0 6,841,034 - -1.2 6,841,135 + -0.9 6,841,135 + +0.0 6,841,135 + -0.4 6,841,135 + +0.6 6,841,135 + +0.7 6,841,135 + +0.4 6,841,135 + +0.2 6,841,136 - +1.0 6,841,198 + +0.4 6,841,198 + -0.3 6,841,509 + -0.7 6,841,509 + -0.4 6,841,509 + +1.3 6,841,509 + +0.7 6,841,509 + -0.3 6,841,653 + Pf6N2E2_6022 0.50 -0.1 6,841,653 + Pf6N2E2_6022 0.50 -0.4 6,841,692 + Pf6N2E2_6022 0.62 +0.6 6,841,787 + -0.5 6,841,788 - -0.3 6,841,865 + +0.7 6,841,866 - +0.6 6,841,866 - +0.2 6,841,866 - +1.0 6,841,866 - +2.1 6,841,928 - Pf6N2E2_6023 0.18 -0.7
Or see this region's nucleotide sequence