Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6016 and Pf6N2E2_6017 are separated by 597 nucleotides Pf6N2E2_6017 and Pf6N2E2_6018 are separated by 102 nucleotides Pf6N2E2_6018 and Pf6N2E2_6019 are separated by 21 nucleotides
Pf6N2E2_6016 - Transcriptional regulator, AraC family, at 6,835,515 to 6,836,414
_6016
Pf6N2E2_6017 - Hexuronate transporter, at 6,837,012 to 6,838,343
_6017
Pf6N2E2_6018 - Fumarylacetoacetate hydrolase family protein, at 6,838,446 to 6,839,144
_6018
Pf6N2E2_6019 - putative oxidoreductase, at 6,839,166 to 6,839,924
_6019
Position (kb)
6837
6838
6839 Strain fitness (log2 ratio)
-2
-1
0
1 at 6836.329 kb on + strand at 6836.329 kb on + strand at 6836.383 kb on + strand at 6836.384 kb on - strand at 6836.384 kb on - strand at 6836.419 kb on + strand at 6836.419 kb on + strand at 6836.419 kb on + strand at 6836.419 kb on + strand at 6836.419 kb on + strand at 6836.420 kb on - strand at 6836.974 kb on + strand at 6836.975 kb on - strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.992 kb on + strand at 6836.993 kb on - strand at 6836.993 kb on - strand at 6837.061 kb on + strand at 6837.062 kb on - strand at 6837.132 kb on + strand at 6837.133 kb on - strand at 6837.149 kb on + strand, within Pf6N2E2_6017 at 6837.149 kb on + strand, within Pf6N2E2_6017 at 6837.150 kb on - strand, within Pf6N2E2_6017 at 6837.150 kb on - strand, within Pf6N2E2_6017 at 6837.150 kb on - strand, within Pf6N2E2_6017 at 6837.297 kb on + strand, within Pf6N2E2_6017 at 6837.480 kb on + strand, within Pf6N2E2_6017 at 6837.481 kb on - strand, within Pf6N2E2_6017 at 6837.481 kb on - strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.568 kb on - strand, within Pf6N2E2_6017 at 6837.619 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.751 kb on - strand, within Pf6N2E2_6017 at 6837.804 kb on + strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.832 kb on - strand, within Pf6N2E2_6017 at 6837.832 kb on - strand, within Pf6N2E2_6017 at 6838.166 kb on + strand, within Pf6N2E2_6017 at 6838.166 kb on + strand, within Pf6N2E2_6017 at 6838.167 kb on - strand, within Pf6N2E2_6017 at 6838.294 kb on - strand at 6838.294 kb on - strand at 6838.317 kb on + strand at 6838.414 kb on - strand at 6838.440 kb on - strand at 6838.461 kb on + strand at 6838.462 kb on - strand at 6838.462 kb on - strand at 6838.462 kb on - strand at 6838.554 kb on + strand, within Pf6N2E2_6018 at 6838.746 kb on + strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.921 kb on + strand, within Pf6N2E2_6018 at 6839.139 kb on + strand at 6839.139 kb on + strand at 6839.253 kb on + strand, within Pf6N2E2_6019
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,836,329 + -0.1 6,836,329 + -0.3 6,836,383 + +0.4 6,836,384 - +1.7 6,836,384 - -1.0 6,836,419 + +0.3 6,836,419 + -0.1 6,836,419 + +0.7 6,836,419 + +0.5 6,836,419 + +0.0 6,836,420 - -1.3 6,836,974 + +0.2 6,836,975 - +0.7 6,836,992 + +1.4 6,836,992 + +0.5 6,836,992 + -0.3 6,836,992 + -2.1 6,836,992 + -0.4 6,836,992 + -1.1 6,836,992 + -0.7 6,836,993 - -1.2 6,836,993 - -1.2 6,837,061 + +0.6 6,837,062 - +0.1 6,837,132 + -0.7 6,837,133 - -0.7 6,837,149 + Pf6N2E2_6017 0.10 +1.3 6,837,149 + Pf6N2E2_6017 0.10 -0.6 6,837,150 - Pf6N2E2_6017 0.10 -0.6 6,837,150 - Pf6N2E2_6017 0.10 +0.4 6,837,150 - Pf6N2E2_6017 0.10 -0.1 6,837,297 + Pf6N2E2_6017 0.21 +1.4 6,837,480 + Pf6N2E2_6017 0.35 -0.1 6,837,481 - Pf6N2E2_6017 0.35 -1.6 6,837,481 - Pf6N2E2_6017 0.35 +0.5 6,837,495 + Pf6N2E2_6017 0.36 -2.1 6,837,495 + Pf6N2E2_6017 0.36 +1.1 6,837,495 + Pf6N2E2_6017 0.36 +0.2 6,837,546 + Pf6N2E2_6017 0.40 -0.1 6,837,546 + Pf6N2E2_6017 0.40 +0.1 6,837,546 + Pf6N2E2_6017 0.40 +0.9 6,837,546 + Pf6N2E2_6017 0.40 -0.5 6,837,547 - Pf6N2E2_6017 0.40 +1.1 6,837,547 - Pf6N2E2_6017 0.40 -1.0 6,837,547 - Pf6N2E2_6017 0.40 +0.5 6,837,547 - Pf6N2E2_6017 0.40 -0.3 6,837,547 - Pf6N2E2_6017 0.40 +0.7 6,837,567 + Pf6N2E2_6017 0.42 -0.8 6,837,567 + Pf6N2E2_6017 0.42 +0.4 6,837,567 + Pf6N2E2_6017 0.42 +0.7 6,837,567 + Pf6N2E2_6017 0.42 -0.2 6,837,568 - Pf6N2E2_6017 0.42 -0.3 6,837,619 - Pf6N2E2_6017 0.46 -0.6 6,837,667 - Pf6N2E2_6017 0.49 +1.7 6,837,667 - Pf6N2E2_6017 0.49 -0.6 6,837,667 - Pf6N2E2_6017 0.49 -0.9 6,837,667 - Pf6N2E2_6017 0.49 -0.5 6,837,667 - Pf6N2E2_6017 0.49 +1.1 6,837,667 - Pf6N2E2_6017 0.49 -1.2 6,837,751 - Pf6N2E2_6017 0.55 +0.6 6,837,804 + Pf6N2E2_6017 0.59 -0.9 6,837,805 - Pf6N2E2_6017 0.60 +0.8 6,837,805 - Pf6N2E2_6017 0.60 +0.8 6,837,805 - Pf6N2E2_6017 0.60 -0.1 6,837,832 - Pf6N2E2_6017 0.62 +0.6 6,837,832 - Pf6N2E2_6017 0.62 -1.9 6,838,166 + Pf6N2E2_6017 0.87 -0.2 6,838,166 + Pf6N2E2_6017 0.87 +0.3 6,838,167 - Pf6N2E2_6017 0.87 +0.3 6,838,294 - +0.9 6,838,294 - -0.1 6,838,317 + -0.8 6,838,414 - +0.0 6,838,440 - +0.4 6,838,461 + -0.6 6,838,462 - +0.4 6,838,462 - -1.1 6,838,462 - -1.8 6,838,554 + Pf6N2E2_6018 0.15 +0.7 6,838,746 + Pf6N2E2_6018 0.43 -0.5 6,838,747 - Pf6N2E2_6018 0.43 -0.8 6,838,747 - Pf6N2E2_6018 0.43 +1.0 6,838,747 - Pf6N2E2_6018 0.43 +0.4 6,838,747 - Pf6N2E2_6018 0.43 +0.3 6,838,747 - Pf6N2E2_6018 0.43 +0.0 6,838,921 + Pf6N2E2_6018 0.68 -0.7 6,839,139 + -0.2 6,839,139 + -1.3 6,839,253 + Pf6N2E2_6019 0.11 +0.6
Or see this region's nucleotide sequence