Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_598 and Pf6N2E2_599 are separated by 93 nucleotides Pf6N2E2_599 and Pf6N2E2_600 overlap by 1 nucleotides Pf6N2E2_600 and Pf6N2E2_601 overlap by 4 nucleotides Pf6N2E2_601 and Pf6N2E2_602 are separated by 45 nucleotides
Pf6N2E2_598 - hypothetical protein, at 673,984 to 674,145
_598
Pf6N2E2_599 - NAD kinase (EC 2.7.1.23), at 674,239 to 675,129
_599
Pf6N2E2_600 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 675,129 to 676,100
_600
Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969
_601
Pf6N2E2_602 - FIG00954117: hypothetical protein, at 677,015 to 677,275
_602
Position (kb) 675
676
677 Strain fitness (log2 ratio) -2
-1
0
1 at 674.178 kb on + strand at 674.197 kb on + strand at 675.423 kb on + strand, within Pf6N2E2_600 at 675.423 kb on + strand, within Pf6N2E2_600 at 675.424 kb on - strand at 675.526 kb on - strand, within Pf6N2E2_600 at 675.933 kb on + strand, within Pf6N2E2_600 at 675.988 kb on - strand, within Pf6N2E2_600 at 676.256 kb on + strand, within Pf6N2E2_601 at 676.257 kb on - strand, within Pf6N2E2_601 at 676.257 kb on - strand, within Pf6N2E2_601 at 676.257 kb on - strand, within Pf6N2E2_601 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.760 kb on + strand, within Pf6N2E2_601 at 676.787 kb on + strand, within Pf6N2E2_601 at 676.954 kb on + strand at 677.060 kb on + strand, within Pf6N2E2_602
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 674,178 + +0.2 674,197 + -2.2 675,423 + Pf6N2E2_600 0.30 -1.1 675,423 + Pf6N2E2_600 0.30 +0.4 675,424 - +0.8 675,526 - Pf6N2E2_600 0.41 -0.6 675,933 + Pf6N2E2_600 0.83 -1.2 675,988 - Pf6N2E2_600 0.88 -0.5 676,256 + Pf6N2E2_601 0.18 -0.4 676,257 - Pf6N2E2_601 0.18 -1.1 676,257 - Pf6N2E2_601 0.18 -0.8 676,257 - Pf6N2E2_601 0.18 -0.7 676,475 + Pf6N2E2_601 0.43 -1.0 676,475 + Pf6N2E2_601 0.43 -0.6 676,476 - Pf6N2E2_601 0.43 +1.3 676,476 - Pf6N2E2_601 0.43 -2.3 676,760 + Pf6N2E2_601 0.76 +0.3 676,787 + Pf6N2E2_601 0.79 +1.2 676,954 + +0.6 677,060 + Pf6N2E2_602 0.17 -0.1
Or see this region's nucleotide sequence