Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5779 and Pf6N2E2_5780 are separated by 118 nucleotides Pf6N2E2_5780 and Pf6N2E2_5781 are separated by 106 nucleotides
Pf6N2E2_5779 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,596,328 to 6,598,115
_5779
Pf6N2E2_5780 - Periplasmic protease, at 6,598,234 to 6,600,345
_5780
Pf6N2E2_5781 - Oxidoreductase, zinc-binding, at 6,600,452 to 6,601,414
_5781
Position (kb)
6598
6599
6600
6601 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6597.415 kb on + strand, within Pf6N2E2_5779 at 6597.415 kb on + strand, within Pf6N2E2_5779 at 6597.595 kb on + strand, within Pf6N2E2_5779 at 6597.595 kb on + strand, within Pf6N2E2_5779 at 6597.634 kb on + strand, within Pf6N2E2_5779 at 6597.912 kb on + strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.913 kb on - strand, within Pf6N2E2_5779 at 6597.951 kb on + strand at 6597.952 kb on - strand at 6598.007 kb on - strand at 6598.166 kb on + strand at 6598.167 kb on - strand at 6598.167 kb on - strand at 6598.268 kb on + strand at 6598.302 kb on - strand at 6598.856 kb on + strand, within Pf6N2E2_5780 at 6598.856 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.934 kb on + strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780 at 6598.935 kb on - strand, within Pf6N2E2_5780 at 6599.150 kb on + strand, within Pf6N2E2_5780 at 6599.189 kb on + strand, within Pf6N2E2_5780 at 6599.190 kb on - strand, within Pf6N2E2_5780 at 6599.190 kb on - strand, within Pf6N2E2_5780 at 6599.190 kb on - strand, within Pf6N2E2_5780 at 6599.190 kb on - strand, within Pf6N2E2_5780 at 6599.190 kb on - strand, within Pf6N2E2_5780 at 6599.222 kb on + strand, within Pf6N2E2_5780 at 6599.222 kb on + strand, within Pf6N2E2_5780 at 6599.223 kb on - strand, within Pf6N2E2_5780 at 6599.223 kb on - strand, within Pf6N2E2_5780 at 6599.624 kb on + strand, within Pf6N2E2_5780 at 6599.625 kb on - strand, within Pf6N2E2_5780 at 6599.642 kb on + strand, within Pf6N2E2_5780 at 6599.666 kb on + strand, within Pf6N2E2_5780 at 6599.666 kb on + strand, within Pf6N2E2_5780 at 6599.666 kb on + strand, within Pf6N2E2_5780 at 6599.666 kb on + strand, within Pf6N2E2_5780 at 6599.667 kb on - strand, within Pf6N2E2_5780 at 6599.883 kb on + strand, within Pf6N2E2_5780 at 6599.931 kb on - strand, within Pf6N2E2_5780 at 6599.969 kb on + strand, within Pf6N2E2_5780 at 6599.969 kb on + strand, within Pf6N2E2_5780 at 6599.969 kb on + strand, within Pf6N2E2_5780 at 6599.970 kb on - strand, within Pf6N2E2_5780 at 6599.970 kb on - strand, within Pf6N2E2_5780 at 6600.065 kb on + strand, within Pf6N2E2_5780 at 6600.095 kb on + strand, within Pf6N2E2_5780 at 6600.095 kb on + strand, within Pf6N2E2_5780 at 6600.096 kb on - strand, within Pf6N2E2_5780 at 6600.096 kb on - strand, within Pf6N2E2_5780 at 6600.104 kb on + strand, within Pf6N2E2_5780 at 6600.146 kb on + strand at 6600.146 kb on + strand at 6600.146 kb on + strand at 6600.146 kb on + strand at 6600.146 kb on + strand at 6600.147 kb on - strand at 6600.278 kb on - strand at 6600.345 kb on + strand at 6600.346 kb on - strand at 6600.346 kb on - strand at 6600.346 kb on - strand at 6600.346 kb on - strand at 6600.348 kb on + strand at 6600.348 kb on + strand at 6600.349 kb on - strand at 6600.349 kb on - strand at 6600.349 kb on - strand at 6600.392 kb on - strand at 6600.392 kb on - strand at 6600.440 kb on + strand at 6600.532 kb on + strand at 6600.532 kb on + strand at 6600.533 kb on - strand at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.750 kb on + strand, within Pf6N2E2_5781 at 6600.751 kb on - strand, within Pf6N2E2_5781 at 6600.751 kb on - strand, within Pf6N2E2_5781 at 6600.751 kb on - strand, within Pf6N2E2_5781 at 6600.751 kb on - strand, within Pf6N2E2_5781 at 6600.751 kb on - strand, within Pf6N2E2_5781 at 6600.806 kb on + strand, within Pf6N2E2_5781 at 6600.864 kb on + strand, within Pf6N2E2_5781 at 6600.971 kb on + strand, within Pf6N2E2_5781 at 6600.971 kb on + strand, within Pf6N2E2_5781 at 6601.005 kb on + strand, within Pf6N2E2_5781 at 6601.005 kb on + strand, within Pf6N2E2_5781 at 6601.080 kb on - strand, within Pf6N2E2_5781 at 6601.189 kb on + strand, within Pf6N2E2_5781
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,597,415 + Pf6N2E2_5779 0.61 +0.3 6,597,415 + Pf6N2E2_5779 0.61 -1.2 6,597,595 + Pf6N2E2_5779 0.71 +0.2 6,597,595 + Pf6N2E2_5779 0.71 +0.1 6,597,634 + Pf6N2E2_5779 0.73 -0.9 6,597,912 + Pf6N2E2_5779 0.89 +0.5 6,597,913 - Pf6N2E2_5779 0.89 -1.2 6,597,913 - Pf6N2E2_5779 0.89 +1.6 6,597,913 - Pf6N2E2_5779 0.89 +1.0 6,597,913 - Pf6N2E2_5779 0.89 -0.8 6,597,913 - Pf6N2E2_5779 0.89 +0.3 6,597,951 + +0.3 6,597,952 - +0.7 6,598,007 - +0.9 6,598,166 + +0.4 6,598,167 - +0.7 6,598,167 - +0.3 6,598,268 + -0.5 6,598,302 - -1.1 6,598,856 + Pf6N2E2_5780 0.29 -0.6 6,598,856 + Pf6N2E2_5780 0.29 -3.6 6,598,934 + Pf6N2E2_5780 0.33 -0.9 6,598,934 + Pf6N2E2_5780 0.33 -1.9 6,598,934 + Pf6N2E2_5780 0.33 -0.7 6,598,935 - Pf6N2E2_5780 0.33 -1.6 6,598,935 - Pf6N2E2_5780 0.33 -1.1 6,598,935 - Pf6N2E2_5780 0.33 -0.8 6,599,150 + Pf6N2E2_5780 0.43 +0.2 6,599,189 + Pf6N2E2_5780 0.45 -1.3 6,599,190 - Pf6N2E2_5780 0.45 -0.6 6,599,190 - Pf6N2E2_5780 0.45 -2.3 6,599,190 - Pf6N2E2_5780 0.45 -2.1 6,599,190 - Pf6N2E2_5780 0.45 -0.7 6,599,190 - Pf6N2E2_5780 0.45 -0.1 6,599,222 + Pf6N2E2_5780 0.47 -1.8 6,599,222 + Pf6N2E2_5780 0.47 -2.1 6,599,223 - Pf6N2E2_5780 0.47 -2.5 6,599,223 - Pf6N2E2_5780 0.47 -0.6 6,599,624 + Pf6N2E2_5780 0.66 +0.6 6,599,625 - Pf6N2E2_5780 0.66 -0.6 6,599,642 + Pf6N2E2_5780 0.67 -0.8 6,599,666 + Pf6N2E2_5780 0.68 -0.5 6,599,666 + Pf6N2E2_5780 0.68 +0.0 6,599,666 + Pf6N2E2_5780 0.68 +0.5 6,599,666 + Pf6N2E2_5780 0.68 -0.1 6,599,667 - Pf6N2E2_5780 0.68 -1.5 6,599,883 + Pf6N2E2_5780 0.78 -3.8 6,599,931 - Pf6N2E2_5780 0.80 +0.8 6,599,969 + Pf6N2E2_5780 0.82 -1.8 6,599,969 + Pf6N2E2_5780 0.82 -0.7 6,599,969 + Pf6N2E2_5780 0.82 -1.4 6,599,970 - Pf6N2E2_5780 0.82 -0.3 6,599,970 - Pf6N2E2_5780 0.82 -1.2 6,600,065 + Pf6N2E2_5780 0.87 +0.5 6,600,095 + Pf6N2E2_5780 0.88 -0.7 6,600,095 + Pf6N2E2_5780 0.88 -0.4 6,600,096 - Pf6N2E2_5780 0.88 -0.5 6,600,096 - Pf6N2E2_5780 0.88 -1.2 6,600,104 + Pf6N2E2_5780 0.89 +0.2 6,600,146 + -1.9 6,600,146 + -0.2 6,600,146 + -1.8 6,600,146 + -3.0 6,600,146 + -1.3 6,600,147 - -1.4 6,600,278 - -0.6 6,600,345 + -2.7 6,600,346 - +1.1 6,600,346 - +0.1 6,600,346 - -0.7 6,600,346 - +1.0 6,600,348 + +0.7 6,600,348 + +0.8 6,600,349 - -0.1 6,600,349 - -2.2 6,600,349 - +1.4 6,600,392 - +0.5 6,600,392 - +0.5 6,600,440 + -1.1 6,600,532 + -0.3 6,600,532 + +0.0 6,600,533 - +0.1 6,600,750 + Pf6N2E2_5781 0.31 +1.0 6,600,750 + Pf6N2E2_5781 0.31 -2.6 6,600,750 + Pf6N2E2_5781 0.31 +0.4 6,600,750 + Pf6N2E2_5781 0.31 +0.2 6,600,750 + Pf6N2E2_5781 0.31 +0.4 6,600,750 + Pf6N2E2_5781 0.31 +1.1 6,600,750 + Pf6N2E2_5781 0.31 +0.4 6,600,750 + Pf6N2E2_5781 0.31 +0.7 6,600,751 - Pf6N2E2_5781 0.31 -1.2 6,600,751 - Pf6N2E2_5781 0.31 +0.2 6,600,751 - Pf6N2E2_5781 0.31 +0.8 6,600,751 - Pf6N2E2_5781 0.31 -0.0 6,600,751 - Pf6N2E2_5781 0.31 +0.4 6,600,806 + Pf6N2E2_5781 0.37 -0.4 6,600,864 + Pf6N2E2_5781 0.43 -1.5 6,600,971 + Pf6N2E2_5781 0.54 -1.2 6,600,971 + Pf6N2E2_5781 0.54 -1.1 6,601,005 + Pf6N2E2_5781 0.57 -0.5 6,601,005 + Pf6N2E2_5781 0.57 -0.5 6,601,080 - Pf6N2E2_5781 0.65 +1.9 6,601,189 + Pf6N2E2_5781 0.77 -0.0
Or see this region's nucleotide sequence