Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5738 and Pf6N2E2_5739 overlap by 4 nucleotides Pf6N2E2_5739 and Pf6N2E2_5740 overlap by 19 nucleotides Pf6N2E2_5740 and Pf6N2E2_5741 overlap by 8 nucleotides Pf6N2E2_5741 and Pf6N2E2_5742 overlap by 4 nucleotides
Pf6N2E2_5738 - Cobyrinic acid A,C-diamide synthase, at 6,554,871 to 6,556,187
_5738
Pf6N2E2_5739 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 6,556,184 to 6,556,834
_5739
Pf6N2E2_5740 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 6,556,816 to 6,557,739
_5740
Pf6N2E2_5741 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 6,557,732 to 6,558,724
_5741
Pf6N2E2_5742 - Cobyric acid synthase (EC 6.3.5.10), at 6,558,721 to 6,560,172
_5742
Position (kb)
6556
6557
6558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6555.839 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.989 kb on + strand, within Pf6N2E2_5738 at 6555.989 kb on + strand, within Pf6N2E2_5738 at 6555.990 kb on - strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6556.044 kb on - strand, within Pf6N2E2_5738 at 6556.104 kb on + strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.226 kb on + strand at 6556.713 kb on + strand, within Pf6N2E2_5739 at 6556.743 kb on - strand, within Pf6N2E2_5739 at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6557.084 kb on - strand, within Pf6N2E2_5740 at 6557.326 kb on + strand, within Pf6N2E2_5740 at 6557.327 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.455 kb on + strand, within Pf6N2E2_5740 at 6557.690 kb on + strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.731 kb on + strand at 6557.731 kb on + strand at 6557.731 kb on + strand at 6557.913 kb on - strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.915 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.931 kb on + strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6557.932 kb on - strand, within Pf6N2E2_5741 at 6558.053 kb on + strand, within Pf6N2E2_5741 at 6558.053 kb on + strand, within Pf6N2E2_5741 at 6558.342 kb on + strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.343 kb on - strand, within Pf6N2E2_5741 at 6558.626 kb on - strand at 6558.697 kb on - strand at 6558.697 kb on - strand at 6558.697 kb on - strand at 6558.704 kb on + strand at 6558.704 kb on + strand at 6558.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,555,839 + Pf6N2E2_5738 0.74 -0.8 6,555,846 + Pf6N2E2_5738 0.74 -0.5 6,555,846 + Pf6N2E2_5738 0.74 +1.2 6,555,846 + Pf6N2E2_5738 0.74 +0.9 6,555,847 - Pf6N2E2_5738 0.74 -0.8 6,555,847 - Pf6N2E2_5738 0.74 +1.1 6,555,847 - Pf6N2E2_5738 0.74 -0.8 6,555,989 + Pf6N2E2_5738 0.85 +0.2 6,555,989 + Pf6N2E2_5738 0.85 -0.9 6,555,990 - Pf6N2E2_5738 0.85 -3.6 6,555,993 + Pf6N2E2_5738 0.85 +0.6 6,555,993 + Pf6N2E2_5738 0.85 -0.0 6,555,993 + Pf6N2E2_5738 0.85 -0.3 6,555,993 + Pf6N2E2_5738 0.85 -1.5 6,555,993 + Pf6N2E2_5738 0.85 +0.6 6,555,993 + Pf6N2E2_5738 0.85 +1.9 6,555,994 - Pf6N2E2_5738 0.85 -0.9 6,555,994 - Pf6N2E2_5738 0.85 -0.4 6,555,994 - Pf6N2E2_5738 0.85 -0.9 6,555,994 - Pf6N2E2_5738 0.85 -2.2 6,555,994 - Pf6N2E2_5738 0.85 -0.1 6,556,044 - Pf6N2E2_5738 0.89 -2.7 6,556,104 + -0.9 6,556,105 - -0.4 6,556,105 - -0.5 6,556,105 - -0.5 6,556,105 - +0.6 6,556,105 - -3.1 6,556,105 - -0.7 6,556,105 - -0.4 6,556,226 + -1.6 6,556,713 + Pf6N2E2_5739 0.81 -1.2 6,556,743 - Pf6N2E2_5739 0.86 +1.1 6,556,805 + -1.6 6,556,805 + -1.1 6,556,805 + -0.3 6,556,805 + -1.6 6,557,084 - Pf6N2E2_5740 0.29 -0.9 6,557,326 + Pf6N2E2_5740 0.55 -1.0 6,557,327 - Pf6N2E2_5740 0.55 -2.2 6,557,341 - Pf6N2E2_5740 0.57 +0.2 6,557,341 - Pf6N2E2_5740 0.57 -1.8 6,557,425 + Pf6N2E2_5740 0.66 +0.2 6,557,425 + Pf6N2E2_5740 0.66 -0.7 6,557,426 - Pf6N2E2_5740 0.66 -0.9 6,557,426 - Pf6N2E2_5740 0.66 -1.6 6,557,426 - Pf6N2E2_5740 0.66 -1.4 6,557,455 + Pf6N2E2_5740 0.69 -1.8 6,557,690 + +0.5 6,557,691 - -0.5 6,557,691 - +0.3 6,557,691 - -3.1 6,557,731 + +0.7 6,557,731 + +0.7 6,557,731 + +0.9 6,557,913 - Pf6N2E2_5741 0.18 -0.1 6,557,915 + Pf6N2E2_5741 0.18 +0.3 6,557,915 + Pf6N2E2_5741 0.18 +0.6 6,557,915 + Pf6N2E2_5741 0.18 -1.0 6,557,915 + Pf6N2E2_5741 0.18 -1.2 6,557,931 + Pf6N2E2_5741 0.20 -1.2 6,557,931 + Pf6N2E2_5741 0.20 +1.4 6,557,931 + Pf6N2E2_5741 0.20 -1.5 6,557,931 + Pf6N2E2_5741 0.20 +1.1 6,557,932 - Pf6N2E2_5741 0.20 -2.0 6,557,932 - Pf6N2E2_5741 0.20 -0.2 6,557,932 - Pf6N2E2_5741 0.20 +0.1 6,558,053 + Pf6N2E2_5741 0.32 +1.4 6,558,053 + Pf6N2E2_5741 0.32 +0.7 6,558,342 + Pf6N2E2_5741 0.61 +1.0 6,558,343 - Pf6N2E2_5741 0.62 -2.3 6,558,343 - Pf6N2E2_5741 0.62 -0.0 6,558,343 - Pf6N2E2_5741 0.62 +0.2 6,558,626 - +0.0 6,558,697 - +0.3 6,558,697 - +0.1 6,558,697 - +0.3 6,558,704 + -2.3 6,558,704 + -0.4 6,558,705 - +1.1
Or see this region's nucleotide sequence