Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5721 and Pf6N2E2_5722 overlap by 1 nucleotides Pf6N2E2_5722 and Pf6N2E2_5723 are separated by 100 nucleotides
Pf6N2E2_5721 - Cysteine synthase B (EC 2.5.1.47), at 6,537,549 to 6,538,451
_5721
Pf6N2E2_5722 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939, at 6,538,451 to 6,539,821
_5722
Pf6N2E2_5723 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 6,539,922 to 6,542,168
_5723
Position (kb)
6538
6539
6540 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6537.460 kb on - strand at 6537.460 kb on - strand at 6537.487 kb on + strand at 6537.510 kb on + strand at 6537.511 kb on - strand at 6537.515 kb on + strand at 6539.506 kb on + strand, within Pf6N2E2_5722 at 6539.532 kb on + strand, within Pf6N2E2_5722 at 6539.660 kb on + strand, within Pf6N2E2_5722 at 6539.660 kb on + strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.664 kb on + strand, within Pf6N2E2_5722 at 6539.664 kb on + strand, within Pf6N2E2_5722 at 6539.665 kb on - strand, within Pf6N2E2_5722 at 6539.749 kb on + strand at 6539.749 kb on + strand at 6539.788 kb on - strand at 6539.855 kb on + strand at 6539.904 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.914 kb on - strand at 6539.917 kb on + strand at 6539.917 kb on + strand at 6539.966 kb on + strand at 6540.074 kb on + strand at 6540.422 kb on + strand, within Pf6N2E2_5723 at 6540.423 kb on - strand, within Pf6N2E2_5723 at 6540.423 kb on - strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.585 kb on + strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.724 kb on - strand, within Pf6N2E2_5723
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,537,460 - +0.2 6,537,460 - -0.0 6,537,487 + -0.3 6,537,510 + +1.0 6,537,511 - -0.6 6,537,515 + -1.1 6,539,506 + Pf6N2E2_5722 0.77 -2.2 6,539,532 + Pf6N2E2_5722 0.79 +2.0 6,539,660 + Pf6N2E2_5722 0.88 +2.3 6,539,660 + Pf6N2E2_5722 0.88 +1.2 6,539,661 - Pf6N2E2_5722 0.88 +1.1 6,539,661 - Pf6N2E2_5722 0.88 +2.4 6,539,661 - Pf6N2E2_5722 0.88 +0.5 6,539,661 - Pf6N2E2_5722 0.88 -0.1 6,539,661 - Pf6N2E2_5722 0.88 +0.2 6,539,661 - Pf6N2E2_5722 0.88 +0.5 6,539,664 + Pf6N2E2_5722 0.88 +0.8 6,539,664 + Pf6N2E2_5722 0.88 +1.9 6,539,665 - Pf6N2E2_5722 0.89 +1.3 6,539,749 + -0.0 6,539,749 + +0.2 6,539,788 - +0.9 6,539,855 + -1.1 6,539,904 + -0.8 6,539,913 + +0.9 6,539,913 + +0.5 6,539,913 + +0.8 6,539,913 + -0.9 6,539,914 - +0.5 6,539,917 + +0.6 6,539,917 + +1.0 6,539,966 + +1.9 6,540,074 + -0.1 6,540,422 + Pf6N2E2_5723 0.22 +1.7 6,540,423 - Pf6N2E2_5723 0.22 +1.1 6,540,423 - Pf6N2E2_5723 0.22 +1.3 6,540,498 + Pf6N2E2_5723 0.26 +0.7 6,540,498 + Pf6N2E2_5723 0.26 +0.9 6,540,498 + Pf6N2E2_5723 0.26 +1.4 6,540,498 + Pf6N2E2_5723 0.26 +0.7 6,540,498 + Pf6N2E2_5723 0.26 +2.3 6,540,498 + Pf6N2E2_5723 0.26 +1.1 6,540,498 + Pf6N2E2_5723 0.26 +2.2 6,540,498 + Pf6N2E2_5723 0.26 +1.3 6,540,498 + Pf6N2E2_5723 0.26 -0.7 6,540,498 + Pf6N2E2_5723 0.26 +0.5 6,540,498 + Pf6N2E2_5723 0.26 -0.6 6,540,498 + Pf6N2E2_5723 0.26 -0.0 6,540,498 + Pf6N2E2_5723 0.26 +1.5 6,540,499 - Pf6N2E2_5723 0.26 +1.4 6,540,499 - Pf6N2E2_5723 0.26 +1.0 6,540,499 - Pf6N2E2_5723 0.26 +2.2 6,540,499 - Pf6N2E2_5723 0.26 -0.1 6,540,499 - Pf6N2E2_5723 0.26 -0.4 6,540,585 + Pf6N2E2_5723 0.30 +1.4 6,540,586 - Pf6N2E2_5723 0.30 +2.1 6,540,586 - Pf6N2E2_5723 0.30 +2.7 6,540,586 - Pf6N2E2_5723 0.30 -1.2 6,540,724 - Pf6N2E2_5723 0.36 +0.5
Or see this region's nucleotide sequence