Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5669 and Pf6N2E2_5670 are separated by 69 nucleotides Pf6N2E2_5670 and Pf6N2E2_5671 are separated by 12 nucleotides Pf6N2E2_5671 and Pf6N2E2_5672 are separated by 56 nucleotides Pf6N2E2_5672 and Pf6N2E2_5673 are separated by 228 nucleotides
Pf6N2E2_5669 - Succinylarginine dihydrolase (EC 3.5.3.23), at 6,482,348 to 6,483,694
_5669
Pf6N2E2_5670 - Putative GTPases (G3E family), at 6,483,764 to 6,484,051
_5670
Pf6N2E2_5671 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 6,484,064 to 6,485,071
_5671
Pf6N2E2_5672 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 6,485,128 to 6,485,637
_5672
Pf6N2E2_5673 - hypothetical protein, at 6,485,866 to 6,486,216
_5673
Position (kb)
6484
6485
6486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6483.137 kb on + strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.283 kb on + strand, within Pf6N2E2_5669 at 6483.365 kb on + strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.659 kb on - strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.758 kb on - strand at 6483.758 kb on - strand at 6483.772 kb on + strand at 6483.773 kb on - strand at 6483.930 kb on - strand, within Pf6N2E2_5670 at 6484.050 kb on - strand at 6484.505 kb on + strand, within Pf6N2E2_5671 at 6484.506 kb on - strand, within Pf6N2E2_5671 at 6484.668 kb on - strand, within Pf6N2E2_5671 at 6484.673 kb on + strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671 at 6484.928 kb on + strand, within Pf6N2E2_5671 at 6484.928 kb on + strand, within Pf6N2E2_5671 at 6484.928 kb on + strand, within Pf6N2E2_5671 at 6485.129 kb on + strand at 6485.185 kb on - strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.487 kb on + strand, within Pf6N2E2_5672 at 6485.696 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.762 kb on - strand at 6485.848 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.885 kb on - strand at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,483,137 + Pf6N2E2_5669 0.59 +1.5 6,483,138 - Pf6N2E2_5669 0.59 -0.4 6,483,138 - Pf6N2E2_5669 0.59 +0.8 6,483,138 - Pf6N2E2_5669 0.59 -1.2 6,483,138 - Pf6N2E2_5669 0.59 +0.2 6,483,283 + Pf6N2E2_5669 0.69 -0.6 6,483,365 + Pf6N2E2_5669 0.76 -1.4 6,483,366 - Pf6N2E2_5669 0.76 -0.2 6,483,366 - Pf6N2E2_5669 0.76 +0.9 6,483,536 + Pf6N2E2_5669 0.88 -0.5 6,483,536 + Pf6N2E2_5669 0.88 -0.8 6,483,659 - +0.1 6,483,713 + +0.9 6,483,713 + -0.7 6,483,713 + +0.0 6,483,757 + +1.6 6,483,757 + +0.8 6,483,757 + +0.4 6,483,758 - -1.3 6,483,758 - -0.4 6,483,772 + +0.5 6,483,773 - +0.2 6,483,930 - Pf6N2E2_5670 0.58 -1.0 6,484,050 - +0.3 6,484,505 + Pf6N2E2_5671 0.44 -2.2 6,484,506 - Pf6N2E2_5671 0.44 +0.3 6,484,668 - Pf6N2E2_5671 0.60 -1.8 6,484,673 + Pf6N2E2_5671 0.60 -2.5 6,484,674 - Pf6N2E2_5671 0.61 -1.6 6,484,674 - Pf6N2E2_5671 0.61 +1.6 6,484,928 + Pf6N2E2_5671 0.86 -1.6 6,484,928 + Pf6N2E2_5671 0.86 -0.8 6,484,928 + Pf6N2E2_5671 0.86 -1.3 6,485,129 + +0.4 6,485,185 - Pf6N2E2_5672 0.11 +0.7 6,485,369 + Pf6N2E2_5672 0.47 -1.4 6,485,369 + Pf6N2E2_5672 0.47 -0.2 6,485,369 + Pf6N2E2_5672 0.47 +0.2 6,485,370 - Pf6N2E2_5672 0.47 +0.3 6,485,370 - Pf6N2E2_5672 0.47 +0.8 6,485,370 - Pf6N2E2_5672 0.47 +0.0 6,485,370 - Pf6N2E2_5672 0.47 -1.2 6,485,370 - Pf6N2E2_5672 0.47 +0.9 6,485,487 + Pf6N2E2_5672 0.70 +0.2 6,485,696 + -0.3 6,485,761 + +0.2 6,485,761 + +0.0 6,485,761 + +1.2 6,485,761 + -0.5 6,485,762 - -0.6 6,485,848 + -1.0 6,485,884 + -0.6 6,485,884 + -0.1 6,485,884 + -0.4 6,485,884 + +2.1 6,485,885 - -0.5 6,485,947 + Pf6N2E2_5673 0.23 +0.8 6,485,947 + Pf6N2E2_5673 0.23 +0.1 6,485,947 + Pf6N2E2_5673 0.23 -0.7 6,485,947 + Pf6N2E2_5673 0.23 +1.3 6,485,947 + Pf6N2E2_5673 0.23 -0.5 6,485,948 - Pf6N2E2_5673 0.23 +2.2 6,485,948 - Pf6N2E2_5673 0.23 -0.9 6,485,948 - Pf6N2E2_5673 0.23 +1.2 6,485,948 - Pf6N2E2_5673 0.23 +1.3
Or see this region's nucleotide sequence