Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_562 and Pf6N2E2_563 are separated by 379 nucleotides Pf6N2E2_563 and Pf6N2E2_564 are separated by 312 nucleotides
Pf6N2E2_562 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 635,624 to 636,577
_562
Pf6N2E2_563 - Serine transporter, at 636,957 to 638,237
_563
Pf6N2E2_564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 638,550 to 641,159
_564
Position (kb) 636
637
638
639 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 636.036 kb on + strand, within Pf6N2E2_562 at 636.069 kb on + strand, within Pf6N2E2_562 at 636.070 kb on - strand, within Pf6N2E2_562 at 636.076 kb on + strand, within Pf6N2E2_562 at 636.222 kb on - strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.534 kb on + strand at 636.535 kb on - strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.591 kb on - strand at 636.591 kb on - strand at 636.612 kb on + strand at 636.613 kb on - strand at 636.613 kb on - strand at 636.627 kb on + strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.659 kb on - strand at 636.661 kb on + strand at 636.661 kb on + strand at 636.690 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.695 kb on - strand at 636.695 kb on - strand at 636.720 kb on + strand at 636.721 kb on - strand at 636.787 kb on + strand at 636.809 kb on + strand at 636.809 kb on + strand at 636.815 kb on + strand at 637.082 kb on + strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.281 kb on + strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.368 kb on - strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.830 kb on + strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 638.121 kb on + strand at 638.122 kb on - strand at 638.122 kb on - strand at 638.221 kb on - strand at 638.345 kb on + strand at 638.346 kb on - strand at 638.445 kb on + strand at 638.494 kb on + strand at 638.494 kb on + strand at 638.495 kb on - strand at 638.495 kb on - strand at 638.545 kb on - strand at 638.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 636,036 + Pf6N2E2_562 0.43 -0.6 636,069 + Pf6N2E2_562 0.47 +1.8 636,070 - Pf6N2E2_562 0.47 -0.6 636,076 + Pf6N2E2_562 0.47 +0.1 636,222 - Pf6N2E2_562 0.63 -0.1 636,335 + Pf6N2E2_562 0.75 -0.3 636,335 + Pf6N2E2_562 0.75 -0.6 636,335 + Pf6N2E2_562 0.75 +2.3 636,335 + Pf6N2E2_562 0.75 +0.5 636,336 - Pf6N2E2_562 0.75 -2.1 636,336 - Pf6N2E2_562 0.75 +0.2 636,336 - Pf6N2E2_562 0.75 -0.7 636,534 + +1.2 636,535 - +0.4 636,590 + +0.2 636,590 + +0.3 636,590 + +0.6 636,590 + +0.1 636,590 + +0.4 636,591 - +1.0 636,591 - +0.5 636,612 + +0.9 636,613 - -1.0 636,613 - +0.8 636,627 + -0.0 636,628 - -0.0 636,628 - +0.5 636,628 - +0.1 636,628 - +0.8 636,658 + -0.3 636,658 + +0.5 636,658 + +0.8 636,658 + +0.1 636,658 + -2.3 636,659 - -1.9 636,661 + -0.5 636,661 + -1.1 636,690 + +0.5 636,694 + -0.5 636,694 + +1.2 636,694 + -0.4 636,695 - -1.2 636,695 - +0.2 636,720 + -1.2 636,721 - +0.4 636,787 + +0.3 636,809 + -2.6 636,809 + +1.8 636,815 + +1.1 637,082 + -1.6 637,083 - -2.6 637,083 - -0.6 637,083 - -0.5 637,167 + Pf6N2E2_563 0.16 -2.9 637,167 + Pf6N2E2_563 0.16 -0.4 637,167 + Pf6N2E2_563 0.16 +0.5 637,168 - Pf6N2E2_563 0.16 +0.8 637,168 - Pf6N2E2_563 0.16 -0.5 637,168 - Pf6N2E2_563 0.16 -0.0 637,168 - Pf6N2E2_563 0.16 +0.6 637,281 + Pf6N2E2_563 0.25 +1.3 637,282 - Pf6N2E2_563 0.25 -0.9 637,282 - Pf6N2E2_563 0.25 +1.1 637,314 + Pf6N2E2_563 0.28 +0.6 637,314 + Pf6N2E2_563 0.28 -0.3 637,315 - Pf6N2E2_563 0.28 +0.1 637,315 - Pf6N2E2_563 0.28 +0.0 637,315 - Pf6N2E2_563 0.28 -1.0 637,368 - Pf6N2E2_563 0.32 +0.1 637,586 + Pf6N2E2_563 0.49 -0.2 637,586 + Pf6N2E2_563 0.49 +0.7 637,587 - Pf6N2E2_563 0.49 +2.0 637,587 - Pf6N2E2_563 0.49 +0.2 637,830 + Pf6N2E2_563 0.68 -1.1 637,921 - Pf6N2E2_563 0.75 -1.6 637,921 - Pf6N2E2_563 0.75 -0.6 637,921 - Pf6N2E2_563 0.75 +0.3 638,121 + -2.2 638,122 - +1.8 638,122 - +1.2 638,221 - -0.1 638,345 + -1.0 638,346 - +0.2 638,445 + -0.1 638,494 + -0.2 638,494 + +0.4 638,495 - +1.0 638,495 - -2.1 638,545 - -0.9 638,545 - +1.6
Or see this region's nucleotide sequence