Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5589 and Pf6N2E2_5590 are separated by 129 nucleotides Pf6N2E2_5590 and Pf6N2E2_5591 are separated by 90 nucleotides
Pf6N2E2_5589 - Sodium-type flagellar protein motY precursor, at 6,406,370 to 6,407,347
_5589
Pf6N2E2_5590 - Argininosuccinate synthase (EC 6.3.4.5), at 6,407,477 to 6,408,694
_5590
Pf6N2E2_5591 - hypothetical protein, at 6,408,785 to 6,409,267
_5591
Position (kb)
6407
6408
6409 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.575 kb on - strand, within Pf6N2E2_5589 at 6406.575 kb on - strand, within Pf6N2E2_5589 at 6406.576 kb on + strand, within Pf6N2E2_5589 at 6406.576 kb on + strand, within Pf6N2E2_5589 at 6406.577 kb on - strand, within Pf6N2E2_5589 at 6406.577 kb on - strand, within Pf6N2E2_5589 at 6406.758 kb on + strand, within Pf6N2E2_5589 at 6406.759 kb on - strand, within Pf6N2E2_5589 at 6407.158 kb on + strand, within Pf6N2E2_5589 at 6407.159 kb on - strand, within Pf6N2E2_5589 at 6407.159 kb on - strand, within Pf6N2E2_5589 at 6407.378 kb on - strand at 6407.378 kb on - strand at 6407.398 kb on + strand at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.835 kb on - strand, within Pf6N2E2_5591 at 6408.835 kb on - strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.910 kb on + strand, within Pf6N2E2_5591 at 6408.910 kb on + strand, within Pf6N2E2_5591 at 6408.911 kb on - strand, within Pf6N2E2_5591 at 6408.920 kb on - strand, within Pf6N2E2_5591 at 6408.920 kb on - strand, within Pf6N2E2_5591 at 6408.950 kb on + strand, within Pf6N2E2_5591 at 6408.950 kb on + strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.986 kb on + strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6409.029 kb on - strand, within Pf6N2E2_5591 at 6409.149 kb on + strand, within Pf6N2E2_5591 at 6409.217 kb on + strand, within Pf6N2E2_5591 at 6409.217 kb on + strand, within Pf6N2E2_5591 at 6409.271 kb on + strand at 6409.385 kb on + strand at 6409.392 kb on + strand at 6409.396 kb on + strand at 6409.491 kb on + strand at 6409.619 kb on + strand at 6409.619 kb on + strand at 6409.626 kb on + strand at 6409.627 kb on - strand at 6409.677 kb on - strand at 6409.691 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,406,574 + Pf6N2E2_5589 0.21 +0.4 6,406,574 + Pf6N2E2_5589 0.21 +0.2 6,406,574 + Pf6N2E2_5589 0.21 +1.7 6,406,574 + Pf6N2E2_5589 0.21 -0.5 6,406,575 - Pf6N2E2_5589 0.21 +1.6 6,406,575 - Pf6N2E2_5589 0.21 +0.1 6,406,576 + Pf6N2E2_5589 0.21 -0.2 6,406,576 + Pf6N2E2_5589 0.21 +0.5 6,406,577 - Pf6N2E2_5589 0.21 +1.3 6,406,577 - Pf6N2E2_5589 0.21 +0.6 6,406,758 + Pf6N2E2_5589 0.40 +0.1 6,406,759 - Pf6N2E2_5589 0.40 +0.7 6,407,158 + Pf6N2E2_5589 0.81 -0.3 6,407,159 - Pf6N2E2_5589 0.81 +0.6 6,407,159 - Pf6N2E2_5589 0.81 -1.5 6,407,378 - -1.7 6,407,378 - -1.5 6,407,398 + -0.2 6,408,825 + +2.0 6,408,825 + +0.3 6,408,825 + +2.0 6,408,825 + +1.2 6,408,826 - -0.3 6,408,826 - -0.1 6,408,826 - +0.4 6,408,826 - +0.0 6,408,826 - -0.0 6,408,834 + Pf6N2E2_5591 0.10 +1.9 6,408,834 + Pf6N2E2_5591 0.10 +0.4 6,408,834 + Pf6N2E2_5591 0.10 -0.0 6,408,834 + Pf6N2E2_5591 0.10 -0.3 6,408,835 - Pf6N2E2_5591 0.10 +2.8 6,408,835 - Pf6N2E2_5591 0.10 +1.9 6,408,905 + Pf6N2E2_5591 0.25 -0.0 6,408,905 + Pf6N2E2_5591 0.25 +0.2 6,408,905 + Pf6N2E2_5591 0.25 +0.0 6,408,905 + Pf6N2E2_5591 0.25 +0.5 6,408,910 + Pf6N2E2_5591 0.26 -0.3 6,408,910 + Pf6N2E2_5591 0.26 +0.2 6,408,911 - Pf6N2E2_5591 0.26 +0.7 6,408,920 - Pf6N2E2_5591 0.28 +0.3 6,408,920 - Pf6N2E2_5591 0.28 -0.0 6,408,950 + Pf6N2E2_5591 0.34 -0.3 6,408,950 + Pf6N2E2_5591 0.34 +1.1 6,408,951 - Pf6N2E2_5591 0.34 -0.9 6,408,951 - Pf6N2E2_5591 0.34 -0.8 6,408,951 - Pf6N2E2_5591 0.34 -1.5 6,408,986 + Pf6N2E2_5591 0.42 -0.5 6,408,987 - Pf6N2E2_5591 0.42 -0.7 6,408,987 - Pf6N2E2_5591 0.42 +0.4 6,408,987 - Pf6N2E2_5591 0.42 -0.0 6,408,987 - Pf6N2E2_5591 0.42 -2.3 6,408,987 - Pf6N2E2_5591 0.42 +0.8 6,409,029 - Pf6N2E2_5591 0.51 +0.1 6,409,149 + Pf6N2E2_5591 0.75 +0.2 6,409,217 + Pf6N2E2_5591 0.89 +1.2 6,409,217 + Pf6N2E2_5591 0.89 -1.6 6,409,271 + -0.7 6,409,385 + +0.3 6,409,392 + +1.2 6,409,396 + -0.3 6,409,491 + +1.4 6,409,619 + -1.1 6,409,619 + -0.2 6,409,626 + +2.3 6,409,627 - +4.9 6,409,677 - -0.1 6,409,691 + -2.3
Or see this region's nucleotide sequence