Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5585 and Pf6N2E2_5586 are separated by 116 nucleotides Pf6N2E2_5586 and Pf6N2E2_5587 are separated by 187 nucleotides Pf6N2E2_5587 and Pf6N2E2_5588 overlap by 4 nucleotides
Pf6N2E2_5585 - hypothetical protein, at 6,403,403 to 6,403,552
_5585
Pf6N2E2_5586 - Alkyl hydroperoxide reductase subunit C-like protein, at 6,403,669 to 6,404,271
_5586
Pf6N2E2_5587 - Ribonuclease T (EC 3.1.13.-), at 6,404,459 to 6,405,136
_5587
Pf6N2E2_5588 - Dihydroorotase (EC 3.5.2.3), at 6,405,133 to 6,406,179
_5588
Position (kb)
6404
6405
6406 Strain fitness (log2 ratio)
-2
-1
0
1 at 6403.563 kb on + strand at 6403.563 kb on + strand at 6403.563 kb on + strand at 6403.563 kb on + strand at 6403.563 kb on + strand at 6403.563 kb on + strand at 6403.564 kb on - strand at 6403.564 kb on - strand at 6403.564 kb on - strand at 6403.564 kb on - strand at 6403.564 kb on - strand at 6403.648 kb on + strand at 6403.649 kb on - strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.651 kb on + strand at 6403.652 kb on - strand at 6403.652 kb on - strand at 6403.652 kb on - strand at 6403.663 kb on + strand at 6403.676 kb on + strand at 6403.677 kb on - strand at 6403.682 kb on + strand at 6403.777 kb on + strand, within Pf6N2E2_5586 at 6403.795 kb on + strand, within Pf6N2E2_5586 at 6403.795 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.969 kb on + strand, within Pf6N2E2_5586 at 6403.970 kb on - strand, within Pf6N2E2_5586 at 6403.970 kb on - strand, within Pf6N2E2_5586 at 6403.970 kb on - strand, within Pf6N2E2_5586 at 6403.970 kb on - strand, within Pf6N2E2_5586 at 6403.970 kb on - strand, within Pf6N2E2_5586 at 6404.011 kb on + strand, within Pf6N2E2_5586 at 6404.011 kb on + strand, within Pf6N2E2_5586 at 6404.011 kb on + strand, within Pf6N2E2_5586 at 6404.011 kb on + strand, within Pf6N2E2_5586 at 6404.011 kb on + strand, within Pf6N2E2_5586 at 6404.115 kb on + strand, within Pf6N2E2_5586 at 6404.115 kb on + strand, within Pf6N2E2_5586 at 6404.116 kb on - strand, within Pf6N2E2_5586 at 6404.242 kb on + strand at 6404.242 kb on + strand at 6404.269 kb on + strand at 6404.269 kb on + strand at 6404.269 kb on + strand at 6404.269 kb on + strand at 6404.269 kb on + strand at 6404.269 kb on + strand at 6404.284 kb on + strand at 6404.284 kb on + strand at 6404.317 kb on + strand at 6404.328 kb on + strand at 6404.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,403,563 + -0.2 6,403,563 + -0.4 6,403,563 + +0.1 6,403,563 + -0.6 6,403,563 + -1.0 6,403,563 + -2.1 6,403,564 - -0.3 6,403,564 - +1.2 6,403,564 - +0.6 6,403,564 - -0.6 6,403,564 - +0.6 6,403,648 + +0.6 6,403,649 - -1.3 6,403,651 + +0.4 6,403,651 + -1.3 6,403,651 + -0.3 6,403,651 + -0.5 6,403,651 + -0.0 6,403,651 + -0.4 6,403,651 + -0.3 6,403,652 - -0.2 6,403,652 - -0.8 6,403,652 - -0.7 6,403,663 + -1.5 6,403,676 + +0.6 6,403,677 - -2.3 6,403,682 + -0.9 6,403,777 + Pf6N2E2_5586 0.18 +1.1 6,403,795 + Pf6N2E2_5586 0.21 -1.4 6,403,795 + Pf6N2E2_5586 0.21 +0.9 6,403,969 + Pf6N2E2_5586 0.50 -0.9 6,403,969 + Pf6N2E2_5586 0.50 -0.5 6,403,969 + Pf6N2E2_5586 0.50 -1.8 6,403,969 + Pf6N2E2_5586 0.50 -1.0 6,403,969 + Pf6N2E2_5586 0.50 -0.9 6,403,969 + Pf6N2E2_5586 0.50 -1.4 6,403,969 + Pf6N2E2_5586 0.50 -1.9 6,403,970 - Pf6N2E2_5586 0.50 -1.6 6,403,970 - Pf6N2E2_5586 0.50 -0.7 6,403,970 - Pf6N2E2_5586 0.50 -1.1 6,403,970 - Pf6N2E2_5586 0.50 -0.8 6,403,970 - Pf6N2E2_5586 0.50 +0.3 6,404,011 + Pf6N2E2_5586 0.57 -0.4 6,404,011 + Pf6N2E2_5586 0.57 -0.3 6,404,011 + Pf6N2E2_5586 0.57 -1.1 6,404,011 + Pf6N2E2_5586 0.57 -0.7 6,404,011 + Pf6N2E2_5586 0.57 -1.8 6,404,115 + Pf6N2E2_5586 0.74 -0.4 6,404,115 + Pf6N2E2_5586 0.74 +0.4 6,404,116 - Pf6N2E2_5586 0.74 -1.4 6,404,242 + -0.5 6,404,242 + +0.1 6,404,269 + -1.1 6,404,269 + -0.5 6,404,269 + -1.5 6,404,269 + +0.1 6,404,269 + -0.7 6,404,269 + -2.0 6,404,284 + -2.3 6,404,284 + -1.5 6,404,317 + +0.5 6,404,328 + +1.1 6,404,329 - +0.2
Or see this region's nucleotide sequence