Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5572 and Pf6N2E2_5573 are separated by 326 nucleotides Pf6N2E2_5573 and Pf6N2E2_5574 are separated by 85 nucleotides
Pf6N2E2_5572 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 6,389,850 to 6,391,388
_5572
Pf6N2E2_5573 - Glycerol-3-phosphate regulon repressor, DeoR family, at 6,391,715 to 6,392,470
_5573
Pf6N2E2_5574 - Glycerol kinase (EC 2.7.1.30), at 6,392,556 to 6,394,061
_5574
Position (kb)
6391
6392
6393 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 6391.433 kb on - strand at 6391.463 kb on - strand at 6391.556 kb on + strand at 6391.610 kb on + strand at 6391.631 kb on - strand at 6391.682 kb on + strand at 6391.682 kb on + strand at 6391.907 kb on + strand, within Pf6N2E2_5573 at 6391.908 kb on - strand, within Pf6N2E2_5573 at 6392.039 kb on + strand, within Pf6N2E2_5573 at 6392.108 kb on - strand, within Pf6N2E2_5573 at 6392.271 kb on + strand, within Pf6N2E2_5573 at 6392.299 kb on - strand, within Pf6N2E2_5573 at 6392.388 kb on - strand, within Pf6N2E2_5573 at 6392.388 kb on - strand, within Pf6N2E2_5573 at 6392.415 kb on + strand at 6392.415 kb on + strand at 6392.547 kb on + strand at 6392.557 kb on + strand at 6392.558 kb on - strand at 6392.734 kb on + strand, within Pf6N2E2_5574 at 6392.734 kb on + strand, within Pf6N2E2_5574 at 6392.734 kb on + strand, within Pf6N2E2_5574 at 6392.734 kb on + strand, within Pf6N2E2_5574 at 6392.735 kb on - strand, within Pf6N2E2_5574 at 6392.735 kb on - strand, within Pf6N2E2_5574 at 6392.735 kb on - strand, within Pf6N2E2_5574 at 6392.735 kb on - strand, within Pf6N2E2_5574 at 6392.895 kb on + strand, within Pf6N2E2_5574 at 6392.896 kb on - strand, within Pf6N2E2_5574 at 6392.896 kb on - strand, within Pf6N2E2_5574 at 6392.911 kb on + strand, within Pf6N2E2_5574 at 6392.955 kb on + strand, within Pf6N2E2_5574 at 6392.993 kb on - strand, within Pf6N2E2_5574 at 6393.004 kb on + strand, within Pf6N2E2_5574 at 6393.040 kb on + strand, within Pf6N2E2_5574 at 6393.076 kb on + strand, within Pf6N2E2_5574 at 6393.274 kb on + strand, within Pf6N2E2_5574 at 6393.275 kb on - strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.380 kb on - strand, within Pf6N2E2_5574
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,391,433 - +3.5 6,391,463 - +1.1 6,391,556 + +1.1 6,391,610 + -1.0 6,391,631 - +2.2 6,391,682 + -1.8 6,391,682 + -2.1 6,391,907 + Pf6N2E2_5573 0.25 -1.3 6,391,908 - Pf6N2E2_5573 0.26 -0.2 6,392,039 + Pf6N2E2_5573 0.43 -2.9 6,392,108 - Pf6N2E2_5573 0.52 -1.5 6,392,271 + Pf6N2E2_5573 0.74 -3.0 6,392,299 - Pf6N2E2_5573 0.77 -1.4 6,392,388 - Pf6N2E2_5573 0.89 -2.0 6,392,388 - Pf6N2E2_5573 0.89 -0.4 6,392,415 + -4.4 6,392,415 + -1.8 6,392,547 + +3.3 6,392,557 + +2.5 6,392,558 - +1.6 6,392,734 + Pf6N2E2_5574 0.12 +5.1 6,392,734 + Pf6N2E2_5574 0.12 +5.0 6,392,734 + Pf6N2E2_5574 0.12 +5.1 6,392,734 + Pf6N2E2_5574 0.12 +5.0 6,392,735 - Pf6N2E2_5574 0.12 +4.6 6,392,735 - Pf6N2E2_5574 0.12 +4.7 6,392,735 - Pf6N2E2_5574 0.12 +4.1 6,392,735 - Pf6N2E2_5574 0.12 +5.3 6,392,895 + Pf6N2E2_5574 0.23 +4.8 6,392,896 - Pf6N2E2_5574 0.23 +5.5 6,392,896 - Pf6N2E2_5574 0.23 +5.1 6,392,911 + Pf6N2E2_5574 0.24 +5.9 6,392,955 + Pf6N2E2_5574 0.26 +4.9 6,392,993 - Pf6N2E2_5574 0.29 +4.3 6,393,004 + Pf6N2E2_5574 0.30 +5.9 6,393,040 + Pf6N2E2_5574 0.32 +5.4 6,393,076 + Pf6N2E2_5574 0.35 +5.9 6,393,274 + Pf6N2E2_5574 0.48 +5.2 6,393,275 - Pf6N2E2_5574 0.48 +4.2 6,393,379 + Pf6N2E2_5574 0.55 +6.1 6,393,379 + Pf6N2E2_5574 0.55 +5.0 6,393,379 + Pf6N2E2_5574 0.55 +5.1 6,393,379 + Pf6N2E2_5574 0.55 +5.3 6,393,379 + Pf6N2E2_5574 0.55 +5.3 6,393,380 - Pf6N2E2_5574 0.55 +5.0
Or see this region's nucleotide sequence