Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5554 and Pf6N2E2_5555 are separated by 93 nucleotides Pf6N2E2_5555 and Pf6N2E2_5556 are separated by 394 nucleotides Pf6N2E2_5556 and Pf6N2E2_5557 are separated by 39 nucleotides
Pf6N2E2_5554 - FIG023103: Predicted transmembrane protein, at 6,370,348 to 6,370,836
_5554
Pf6N2E2_5555 - Cold shock protein CspA, at 6,370,930 to 6,371,142
_5555
Pf6N2E2_5556 - Aminomethyltransferase (EC 2.1.2.10) (from data) , at 6,371,537 to 6,372,661
_5556
Pf6N2E2_5557 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 6,372,701 to 6,374,077
_5557
Position (kb)
6371
6372
6373 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6370.712 kb on + strand, within Pf6N2E2_5554 at 6370.712 kb on + strand, within Pf6N2E2_5554 at 6370.712 kb on + strand, within Pf6N2E2_5554 at 6370.726 kb on - strand, within Pf6N2E2_5554 at 6370.760 kb on + strand, within Pf6N2E2_5554 at 6370.761 kb on - strand, within Pf6N2E2_5554 at 6370.874 kb on + strand at 6370.874 kb on + strand at 6370.874 kb on + strand at 6370.883 kb on - strand at 6370.898 kb on - strand at 6370.898 kb on - strand at 6370.915 kb on + strand at 6370.932 kb on - strand at 6370.942 kb on + strand at 6370.943 kb on - strand at 6371.083 kb on - strand, within Pf6N2E2_5555 at 6371.083 kb on - strand, within Pf6N2E2_5555 at 6371.197 kb on + strand at 6371.198 kb on - strand at 6371.220 kb on + strand at 6371.220 kb on + strand at 6371.220 kb on + strand at 6371.221 kb on - strand at 6371.221 kb on - strand at 6371.221 kb on - strand at 6371.221 kb on - strand at 6371.221 kb on - strand at 6371.241 kb on + strand at 6371.241 kb on + strand at 6371.242 kb on - strand at 6371.253 kb on + strand at 6371.253 kb on + strand at 6371.253 kb on + strand at 6371.254 kb on - strand at 6371.254 kb on - strand at 6371.254 kb on - strand at 6371.254 kb on - strand at 6371.258 kb on + strand at 6371.320 kb on + strand at 6371.377 kb on + strand at 6371.377 kb on + strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.378 kb on - strand at 6371.381 kb on + strand at 6371.381 kb on + strand at 6371.382 kb on - strand at 6371.382 kb on - strand at 6371.382 kb on - strand at 6371.401 kb on - strand at 6371.401 kb on - strand at 6371.413 kb on - strand at 6371.413 kb on - strand at 6371.413 kb on - strand at 6371.434 kb on + strand at 6371.434 kb on + strand at 6371.435 kb on - strand at 6371.435 kb on - strand at 6371.437 kb on - strand at 6371.437 kb on - strand at 6371.454 kb on + strand at 6371.454 kb on + strand at 6371.454 kb on + strand at 6371.487 kb on - strand at 6371.502 kb on + strand at 6371.503 kb on - strand at 6371.503 kb on - strand at 6371.519 kb on - strand at 6371.524 kb on + strand at 6371.525 kb on - strand at 6371.550 kb on + strand at 6371.550 kb on + strand at 6371.551 kb on - strand at 6371.580 kb on - strand at 6371.585 kb on + strand at 6371.585 kb on + strand at 6371.585 kb on + strand at 6371.586 kb on - strand at 6371.640 kb on + strand at 6371.640 kb on + strand at 6371.641 kb on - strand at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.765 kb on - strand, within Pf6N2E2_5556 at 6371.765 kb on - strand, within Pf6N2E2_5556 at 6371.876 kb on + strand, within Pf6N2E2_5556 at 6371.943 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.580 kb on - strand at 6372.598 kb on + strand at 6372.599 kb on - strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.750 kb on + strand at 6372.750 kb on + strand at 6372.750 kb on + strand at 6372.751 kb on - strand at 6372.751 kb on - strand at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.170 kb on + strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.210 kb on + strand, within Pf6N2E2_5557 at 6373.266 kb on + strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.519 kb on - strand, within Pf6N2E2_5557 at 6373.644 kb on + strand, within Pf6N2E2_5557 at 6373.644 kb on + strand, within Pf6N2E2_5557 at 6373.647 kb on + strand, within Pf6N2E2_5557
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,370,712 + Pf6N2E2_5554 0.74 +0.8 6,370,712 + Pf6N2E2_5554 0.74 -1.0 6,370,712 + Pf6N2E2_5554 0.74 +1.3 6,370,726 - Pf6N2E2_5554 0.77 +0.2 6,370,760 + Pf6N2E2_5554 0.84 +0.6 6,370,761 - Pf6N2E2_5554 0.84 -0.4 6,370,874 + +0.2 6,370,874 + -0.5 6,370,874 + -1.0 6,370,883 - -2.0 6,370,898 - -1.1 6,370,898 - -3.0 6,370,915 + +0.1 6,370,932 - -0.3 6,370,942 + -0.4 6,370,943 - -1.6 6,371,083 - Pf6N2E2_5555 0.72 -1.1 6,371,083 - Pf6N2E2_5555 0.72 -1.9 6,371,197 + +0.2 6,371,198 - +2.1 6,371,220 + -1.2 6,371,220 + -0.2 6,371,220 + +0.7 6,371,221 - -0.4 6,371,221 - +1.3 6,371,221 - +0.1 6,371,221 - +0.4 6,371,221 - +0.5 6,371,241 + -0.1 6,371,241 + +0.1 6,371,242 - -2.1 6,371,253 + +0.2 6,371,253 + -1.1 6,371,253 + +0.4 6,371,254 - +0.1 6,371,254 - -0.9 6,371,254 - -0.2 6,371,254 - -0.5 6,371,258 + +0.6 6,371,320 + -1.1 6,371,377 + -0.1 6,371,377 + +1.9 6,371,378 - -2.2 6,371,378 - -0.0 6,371,378 - +0.6 6,371,378 - +1.2 6,371,378 - +1.3 6,371,378 - -0.3 6,371,378 - +0.3 6,371,378 - -0.3 6,371,378 - -0.0 6,371,378 - +0.7 6,371,378 - -0.7 6,371,381 + -1.2 6,371,381 + +0.5 6,371,382 - +0.3 6,371,382 - -0.9 6,371,382 - +0.2 6,371,401 - +1.2 6,371,401 - +0.5 6,371,413 - -0.9 6,371,413 - +0.0 6,371,413 - +0.9 6,371,434 + +0.7 6,371,434 + +0.6 6,371,435 - -0.0 6,371,435 - +0.8 6,371,437 - +0.4 6,371,437 - +1.3 6,371,454 + +0.5 6,371,454 + +0.2 6,371,454 + -0.1 6,371,487 - +1.2 6,371,502 + +0.1 6,371,503 - +0.8 6,371,503 - -0.1 6,371,519 - -1.1 6,371,524 + +0.0 6,371,525 - -0.3 6,371,550 + -0.1 6,371,550 + -0.1 6,371,551 - -0.0 6,371,580 - +0.5 6,371,585 + +0.2 6,371,585 + +1.5 6,371,585 + -1.9 6,371,586 - -0.3 6,371,640 + -0.4 6,371,640 + -1.1 6,371,641 - -0.2 6,371,764 + Pf6N2E2_5556 0.20 -0.6 6,371,764 + Pf6N2E2_5556 0.20 +0.5 6,371,764 + Pf6N2E2_5556 0.20 -0.8 6,371,764 + Pf6N2E2_5556 0.20 +0.1 6,371,765 - Pf6N2E2_5556 0.20 +1.1 6,371,765 - Pf6N2E2_5556 0.20 -0.0 6,371,876 + Pf6N2E2_5556 0.30 -1.7 6,371,943 + Pf6N2E2_5556 0.36 +1.2 6,372,105 + Pf6N2E2_5556 0.50 -0.1 6,372,105 + Pf6N2E2_5556 0.50 -0.0 6,372,105 + Pf6N2E2_5556 0.50 +0.0 6,372,105 + Pf6N2E2_5556 0.50 -0.1 6,372,106 - Pf6N2E2_5556 0.51 +0.5 6,372,106 - Pf6N2E2_5556 0.51 -1.7 6,372,106 - Pf6N2E2_5556 0.51 -0.5 6,372,106 - Pf6N2E2_5556 0.51 +0.1 6,372,580 - -0.8 6,372,598 + -0.7 6,372,599 - +0.7 6,372,661 + +0.2 6,372,661 + -0.0 6,372,661 + +0.6 6,372,661 + +0.6 6,372,661 + +0.6 6,372,662 - +0.2 6,372,662 - -0.5 6,372,662 - -3.1 6,372,662 - -0.8 6,372,662 - +0.9 6,372,662 - -0.4 6,372,662 - -0.5 6,372,750 + -0.1 6,372,750 + -1.3 6,372,750 + +0.8 6,372,751 - -0.4 6,372,751 - +1.8 6,373,089 + Pf6N2E2_5557 0.28 +0.4 6,373,089 + Pf6N2E2_5557 0.28 -0.1 6,373,089 + Pf6N2E2_5557 0.28 +0.8 6,373,090 - Pf6N2E2_5557 0.28 +0.3 6,373,090 - Pf6N2E2_5557 0.28 +0.4 6,373,090 - Pf6N2E2_5557 0.28 -0.1 6,373,090 - Pf6N2E2_5557 0.28 -0.3 6,373,090 - Pf6N2E2_5557 0.28 -0.5 6,373,167 + Pf6N2E2_5557 0.34 +0.4 6,373,167 + Pf6N2E2_5557 0.34 -0.2 6,373,167 + Pf6N2E2_5557 0.34 -1.9 6,373,167 + Pf6N2E2_5557 0.34 +1.0 6,373,167 + Pf6N2E2_5557 0.34 -0.6 6,373,167 + Pf6N2E2_5557 0.34 -0.6 6,373,167 + Pf6N2E2_5557 0.34 +1.4 6,373,167 + Pf6N2E2_5557 0.34 +0.9 6,373,168 - Pf6N2E2_5557 0.34 -1.4 6,373,168 - Pf6N2E2_5557 0.34 -0.1 6,373,168 - Pf6N2E2_5557 0.34 -1.2 6,373,168 - Pf6N2E2_5557 0.34 -0.4 6,373,168 - Pf6N2E2_5557 0.34 +0.7 6,373,168 - Pf6N2E2_5557 0.34 +0.9 6,373,168 - Pf6N2E2_5557 0.34 -0.1 6,373,170 + Pf6N2E2_5557 0.34 -0.4 6,373,171 - Pf6N2E2_5557 0.34 -1.0 6,373,171 - Pf6N2E2_5557 0.34 -0.1 6,373,171 - Pf6N2E2_5557 0.34 +0.7 6,373,171 - Pf6N2E2_5557 0.34 +0.8 6,373,171 - Pf6N2E2_5557 0.34 -1.2 6,373,171 - Pf6N2E2_5557 0.34 +0.5 6,373,171 - Pf6N2E2_5557 0.34 +0.7 6,373,210 + Pf6N2E2_5557 0.37 -0.9 6,373,266 + Pf6N2E2_5557 0.41 +0.8 6,373,267 - Pf6N2E2_5557 0.41 -0.1 6,373,267 - Pf6N2E2_5557 0.41 -1.4 6,373,267 - Pf6N2E2_5557 0.41 -0.1 6,373,518 + Pf6N2E2_5557 0.59 -0.2 6,373,518 + Pf6N2E2_5557 0.59 +0.5 6,373,518 + Pf6N2E2_5557 0.59 -0.5 6,373,518 + Pf6N2E2_5557 0.59 +0.6 6,373,518 + Pf6N2E2_5557 0.59 +0.1 6,373,518 + Pf6N2E2_5557 0.59 -1.0 6,373,518 + Pf6N2E2_5557 0.59 -0.3 6,373,519 - Pf6N2E2_5557 0.59 -0.0 6,373,644 + Pf6N2E2_5557 0.68 -2.1 6,373,644 + Pf6N2E2_5557 0.68 -0.9 6,373,647 + Pf6N2E2_5557 0.69 +0.2
Or see this region's nucleotide sequence