Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5529 and Pf6N2E2_5530 are separated by 302 nucleotides Pf6N2E2_5530 and Pf6N2E2_5531 are separated by 3 nucleotides Pf6N2E2_5531 and Pf6N2E2_5532 are separated by 21 nucleotides Pf6N2E2_5532 and Pf6N2E2_5533 are separated by 75 nucleotides
Pf6N2E2_5529 - Non-specific DNA-binding protein Dps / Iron-binding ferritin-like antioxidant protein / Ferroxidase (EC 1.16.3.1), at 6,347,486 to 6,347,956
_5529
Pf6N2E2_5530 - FIG00955846: hypothetical protein, at 6,348,259 to 6,348,603
_5530
Pf6N2E2_5531 - hypothetical protein, at 6,348,607 to 6,348,771
_5531
Pf6N2E2_5532 - Glycerate kinase (EC 2.7.1.31), at 6,348,793 to 6,348,972
_5532
Pf6N2E2_5533 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 6,349,048 to 6,350,823
_5533
Position (kb)
6348
6349 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6347.830 kb on + strand, within Pf6N2E2_5529 at 6347.854 kb on - strand, within Pf6N2E2_5529 at 6347.954 kb on + strand at 6347.954 kb on + strand at 6347.954 kb on + strand at 6347.955 kb on - strand at 6347.958 kb on - strand at 6347.958 kb on - strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6348.105 kb on + strand at 6348.105 kb on + strand at 6348.105 kb on + strand at 6348.106 kb on - strand at 6348.115 kb on + strand at 6348.115 kb on + strand at 6348.115 kb on + strand at 6348.116 kb on - strand at 6348.147 kb on + strand at 6348.147 kb on + strand at 6348.161 kb on + strand at 6348.162 kb on - strand at 6348.236 kb on + strand at 6348.236 kb on + strand at 6348.258 kb on + strand at 6348.258 kb on + strand at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.419 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.608 kb on + strand at 6348.609 kb on - strand at 6348.658 kb on + strand, within Pf6N2E2_5531 at 6348.679 kb on + strand, within Pf6N2E2_5531 at 6348.679 kb on + strand, within Pf6N2E2_5531 at 6348.686 kb on + strand, within Pf6N2E2_5531 at 6348.686 kb on + strand, within Pf6N2E2_5531 at 6348.688 kb on + strand, within Pf6N2E2_5531 at 6348.688 kb on + strand, within Pf6N2E2_5531 at 6348.728 kb on + strand, within Pf6N2E2_5531 at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.766 kb on + strand at 6348.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,347,830 + Pf6N2E2_5529 0.73 -1.2 6,347,854 - Pf6N2E2_5529 0.78 -2.1 6,347,954 + -0.8 6,347,954 + -0.5 6,347,954 + +1.3 6,347,955 - +0.1 6,347,958 - -0.9 6,347,958 - -0.5 6,347,971 + -2.1 6,347,971 + +1.4 6,347,971 + -0.1 6,347,971 + +1.5 6,347,971 + -0.8 6,347,971 + -0.4 6,347,971 + +0.4 6,347,971 + -1.7 6,347,971 + +0.2 6,347,972 - +0.3 6,347,972 - +0.7 6,347,972 - +0.1 6,347,972 - +0.6 6,347,972 - +1.8 6,347,972 - +0.1 6,347,972 - +0.7 6,348,105 + -0.8 6,348,105 + +1.1 6,348,105 + -0.1 6,348,106 - +0.1 6,348,115 + +0.9 6,348,115 + +0.6 6,348,115 + -0.9 6,348,116 - -1.7 6,348,147 + -1.8 6,348,147 + +0.1 6,348,161 + +1.0 6,348,162 - +1.3 6,348,236 + +1.0 6,348,236 + +1.0 6,348,258 + +0.0 6,348,258 + +1.1 6,348,418 + Pf6N2E2_5530 0.46 -1.1 6,348,418 + Pf6N2E2_5530 0.46 -0.3 6,348,418 + Pf6N2E2_5530 0.46 +0.4 6,348,418 + Pf6N2E2_5530 0.46 +0.1 6,348,419 - Pf6N2E2_5530 0.46 -1.7 6,348,469 - Pf6N2E2_5530 0.61 +0.4 6,348,469 - Pf6N2E2_5530 0.61 +1.0 6,348,469 - Pf6N2E2_5530 0.61 -0.3 6,348,469 - Pf6N2E2_5530 0.61 +0.1 6,348,548 + Pf6N2E2_5530 0.84 -0.8 6,348,548 + Pf6N2E2_5530 0.84 +0.2 6,348,548 + Pf6N2E2_5530 0.84 +0.6 6,348,548 + Pf6N2E2_5530 0.84 +1.4 6,348,548 + Pf6N2E2_5530 0.84 +0.8 6,348,548 + Pf6N2E2_5530 0.84 +0.8 6,348,548 + Pf6N2E2_5530 0.84 -1.1 6,348,548 + Pf6N2E2_5530 0.84 -0.0 6,348,548 + Pf6N2E2_5530 0.84 -0.1 6,348,548 + Pf6N2E2_5530 0.84 +0.5 6,348,548 + Pf6N2E2_5530 0.84 -1.7 6,348,549 - Pf6N2E2_5530 0.84 +1.5 6,348,549 - Pf6N2E2_5530 0.84 +0.4 6,348,549 - Pf6N2E2_5530 0.84 -1.5 6,348,549 - Pf6N2E2_5530 0.84 +0.4 6,348,549 - Pf6N2E2_5530 0.84 -0.4 6,348,549 - Pf6N2E2_5530 0.84 -0.9 6,348,549 - Pf6N2E2_5530 0.84 -0.2 6,348,549 - Pf6N2E2_5530 0.84 +0.4 6,348,608 + +2.0 6,348,609 - +3.8 6,348,658 + Pf6N2E2_5531 0.31 +0.8 6,348,679 + Pf6N2E2_5531 0.44 +1.3 6,348,679 + Pf6N2E2_5531 0.44 -0.7 6,348,686 + Pf6N2E2_5531 0.48 +0.6 6,348,686 + Pf6N2E2_5531 0.48 +0.4 6,348,688 + Pf6N2E2_5531 0.49 +0.8 6,348,688 + Pf6N2E2_5531 0.49 +0.1 6,348,728 + Pf6N2E2_5531 0.73 +1.6 6,348,760 + +0.3 6,348,760 + -0.4 6,348,760 + +0.3 6,348,760 + +1.0 6,348,760 + +0.2 6,348,760 + -0.3 6,348,760 + -0.3 6,348,760 + -0.7 6,348,760 + -0.6 6,348,766 + -0.9 6,348,766 + -0.1
Or see this region's nucleotide sequence