Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5524 and Pf6N2E2_5525 are separated by 888 nucleotides Pf6N2E2_5525 and Pf6N2E2_5526 are separated by 3 nucleotides Pf6N2E2_5526 and Pf6N2E2_5527 are separated by 78 nucleotides
Pf6N2E2_5524 - Outer membrane porin, OprD family, at 6,343,630 to 6,344,883
_5524
Pf6N2E2_5525 - Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 6,345,772 to 6,346,197
_5525
Pf6N2E2_5526 - Protein SlyX, at 6,346,201 to 6,346,407
_5526
Pf6N2E2_5527 - Cold shock protein CspC, at 6,346,486 to 6,347,091
_5527
Position (kb)
6345
6346
6347 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6344.788 kb on - strand at 6344.915 kb on - strand at 6344.976 kb on - strand at 6344.976 kb on - strand at 6344.976 kb on - strand at 6344.976 kb on - strand at 6344.977 kb on + strand at 6344.978 kb on - strand at 6344.978 kb on - strand at 6344.978 kb on - strand at 6345.100 kb on + strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.101 kb on - strand at 6345.131 kb on + strand at 6345.132 kb on - strand at 6345.132 kb on - strand at 6345.132 kb on - strand at 6345.132 kb on - strand at 6345.132 kb on - strand at 6345.191 kb on + strand at 6345.191 kb on + strand at 6345.192 kb on - strand at 6345.429 kb on + strand at 6345.469 kb on + strand at 6345.522 kb on + strand at 6345.523 kb on - strand at 6345.523 kb on - strand at 6345.663 kb on + strand at 6345.663 kb on + strand at 6345.724 kb on + strand at 6345.725 kb on - strand at 6345.725 kb on - strand at 6345.725 kb on - strand at 6345.764 kb on + strand at 6345.765 kb on - strand at 6345.765 kb on - strand at 6345.782 kb on + strand at 6345.862 kb on + strand, within Pf6N2E2_5525 at 6345.863 kb on - strand, within Pf6N2E2_5525 at 6345.897 kb on + strand, within Pf6N2E2_5525 at 6345.898 kb on - strand, within Pf6N2E2_5525 at 6346.059 kb on + strand, within Pf6N2E2_5525 at 6346.060 kb on - strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.117 kb on + strand, within Pf6N2E2_5525 at 6346.118 kb on - strand, within Pf6N2E2_5525 at 6346.118 kb on - strand, within Pf6N2E2_5525 at 6346.118 kb on - strand, within Pf6N2E2_5525 at 6346.118 kb on - strand, within Pf6N2E2_5525 at 6346.118 kb on - strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.119 kb on + strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.120 kb on - strand, within Pf6N2E2_5525 at 6346.402 kb on + strand at 6346.402 kb on + strand at 6346.402 kb on + strand at 6346.403 kb on - strand at 6346.436 kb on + strand at 6346.436 kb on + strand at 6346.669 kb on - strand, within Pf6N2E2_5527 at 6346.713 kb on - strand, within Pf6N2E2_5527 at 6346.982 kb on + strand, within Pf6N2E2_5527 at 6347.006 kb on + strand, within Pf6N2E2_5527 at 6347.178 kb on + strand at 6347.193 kb on - strand at 6347.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,344,788 - +1.3 6,344,915 - +0.3 6,344,976 - -0.5 6,344,976 - +0.3 6,344,976 - +0.4 6,344,976 - +0.8 6,344,977 + -1.3 6,344,978 - -0.4 6,344,978 - +1.3 6,344,978 - -0.3 6,345,100 + +0.8 6,345,101 - +1.0 6,345,101 - +0.5 6,345,101 - +0.6 6,345,101 - +0.5 6,345,101 - +0.5 6,345,101 - -0.7 6,345,101 - +1.5 6,345,101 - +0.4 6,345,101 - +0.7 6,345,131 + -0.1 6,345,132 - +0.3 6,345,132 - +0.2 6,345,132 - +0.9 6,345,132 - +0.5 6,345,132 - +0.7 6,345,191 + -2.0 6,345,191 + +1.5 6,345,192 - +0.5 6,345,429 + +0.3 6,345,469 + -0.4 6,345,522 + +0.0 6,345,523 - +1.7 6,345,523 - +1.3 6,345,663 + +0.2 6,345,663 + -0.8 6,345,724 + -0.4 6,345,725 - -1.5 6,345,725 - +1.2 6,345,725 - -1.5 6,345,764 + -0.5 6,345,765 - -1.5 6,345,765 - -1.2 6,345,782 + -1.9 6,345,862 + Pf6N2E2_5525 0.21 -1.9 6,345,863 - Pf6N2E2_5525 0.21 -1.2 6,345,897 + Pf6N2E2_5525 0.29 -2.2 6,345,898 - Pf6N2E2_5525 0.30 -1.8 6,346,059 + Pf6N2E2_5525 0.67 -1.1 6,346,060 - Pf6N2E2_5525 0.68 -3.7 6,346,117 + Pf6N2E2_5525 0.81 -1.4 6,346,117 + Pf6N2E2_5525 0.81 -0.6 6,346,117 + Pf6N2E2_5525 0.81 -0.5 6,346,117 + Pf6N2E2_5525 0.81 -1.1 6,346,117 + Pf6N2E2_5525 0.81 -1.3 6,346,117 + Pf6N2E2_5525 0.81 -0.3 6,346,118 - Pf6N2E2_5525 0.81 -2.0 6,346,118 - Pf6N2E2_5525 0.81 +0.5 6,346,118 - Pf6N2E2_5525 0.81 -2.0 6,346,118 - Pf6N2E2_5525 0.81 +0.8 6,346,118 - Pf6N2E2_5525 0.81 -2.4 6,346,119 + Pf6N2E2_5525 0.81 -0.8 6,346,119 + Pf6N2E2_5525 0.81 -2.7 6,346,119 + Pf6N2E2_5525 0.81 -0.4 6,346,119 + Pf6N2E2_5525 0.81 +0.1 6,346,119 + Pf6N2E2_5525 0.81 -0.1 6,346,119 + Pf6N2E2_5525 0.81 -0.9 6,346,119 + Pf6N2E2_5525 0.81 -1.4 6,346,119 + Pf6N2E2_5525 0.81 -2.2 6,346,119 + Pf6N2E2_5525 0.81 -2.9 6,346,119 + Pf6N2E2_5525 0.81 -1.0 6,346,119 + Pf6N2E2_5525 0.81 -0.2 6,346,120 - Pf6N2E2_5525 0.82 -0.2 6,346,120 - Pf6N2E2_5525 0.82 -0.9 6,346,120 - Pf6N2E2_5525 0.82 -0.4 6,346,120 - Pf6N2E2_5525 0.82 -0.2 6,346,120 - Pf6N2E2_5525 0.82 +0.3 6,346,120 - Pf6N2E2_5525 0.82 +0.2 6,346,120 - Pf6N2E2_5525 0.82 -1.1 6,346,120 - Pf6N2E2_5525 0.82 -0.2 6,346,120 - Pf6N2E2_5525 0.82 -0.8 6,346,120 - Pf6N2E2_5525 0.82 -0.4 6,346,120 - Pf6N2E2_5525 0.82 -1.7 6,346,120 - Pf6N2E2_5525 0.82 -2.1 6,346,120 - Pf6N2E2_5525 0.82 -0.5 6,346,120 - Pf6N2E2_5525 0.82 +0.1 6,346,402 + +0.4 6,346,402 + -0.2 6,346,402 + -0.0 6,346,403 - -0.8 6,346,436 + -1.9 6,346,436 + +0.2 6,346,669 - Pf6N2E2_5527 0.30 -0.7 6,346,713 - Pf6N2E2_5527 0.37 -1.9 6,346,982 + Pf6N2E2_5527 0.82 -0.7 6,347,006 + Pf6N2E2_5527 0.86 -0.8 6,347,178 + -1.8 6,347,193 - +0.5 6,347,193 - -0.5
Or see this region's nucleotide sequence