Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5515 and Pf6N2E2_5516 are separated by 123 nucleotides Pf6N2E2_5516 and Pf6N2E2_5517 are separated by 93 nucleotides Pf6N2E2_5517 and Pf6N2E2_5518 are separated by 11 nucleotides
Pf6N2E2_5515 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 6,332,373 to 6,335,108
_5515
Pf6N2E2_5516 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 6,335,232 to 6,336,449
_5516
Pf6N2E2_5517 - Glutathione S-transferase (EC 2.5.1.18), at 6,336,543 to 6,337,175
_5517
Pf6N2E2_5518 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 6,337,187 to 6,338,116
_5518
Position (kb)
6335
6336
6337 Strain fitness (log2 ratio)
-2
-1
0
1 at 6335.079 kb on + strand at 6335.219 kb on + strand at 6336.304 kb on - strand, within Pf6N2E2_5516 at 6336.447 kb on + strand at 6336.447 kb on + strand at 6336.448 kb on - strand at 6336.525 kb on + strand at 6336.526 kb on - strand at 6336.526 kb on - strand at 6336.526 kb on - strand at 6336.630 kb on + strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.631 kb on - strand, within Pf6N2E2_5517 at 6336.688 kb on - strand, within Pf6N2E2_5517 at 6336.688 kb on - strand, within Pf6N2E2_5517 at 6336.762 kb on + strand, within Pf6N2E2_5517 at 6337.002 kb on + strand, within Pf6N2E2_5517 at 6337.002 kb on + strand, within Pf6N2E2_5517 at 6337.003 kb on - strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.068 kb on + strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.069 kb on - strand, within Pf6N2E2_5517 at 6337.101 kb on + strand, within Pf6N2E2_5517 at 6337.101 kb on + strand, within Pf6N2E2_5517 at 6337.102 kb on - strand, within Pf6N2E2_5517 at 6337.116 kb on + strand at 6337.394 kb on + strand, within Pf6N2E2_5518
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,335,079 + -0.1 6,335,219 + -2.1 6,336,304 - Pf6N2E2_5516 0.88 -2.1 6,336,447 + -0.1 6,336,447 + -1.6 6,336,448 - -0.3 6,336,525 + +0.1 6,336,526 - +1.3 6,336,526 - +1.5 6,336,526 - +0.9 6,336,630 + Pf6N2E2_5517 0.14 -0.1 6,336,631 - Pf6N2E2_5517 0.14 -0.3 6,336,631 - Pf6N2E2_5517 0.14 -0.4 6,336,631 - Pf6N2E2_5517 0.14 +0.7 6,336,688 - Pf6N2E2_5517 0.23 -0.9 6,336,688 - Pf6N2E2_5517 0.23 +0.3 6,336,762 + Pf6N2E2_5517 0.35 -0.1 6,337,002 + Pf6N2E2_5517 0.73 +0.1 6,337,002 + Pf6N2E2_5517 0.73 +1.0 6,337,003 - Pf6N2E2_5517 0.73 +0.7 6,337,068 + Pf6N2E2_5517 0.83 -0.7 6,337,068 + Pf6N2E2_5517 0.83 -0.3 6,337,068 + Pf6N2E2_5517 0.83 +0.0 6,337,068 + Pf6N2E2_5517 0.83 -1.2 6,337,069 - Pf6N2E2_5517 0.83 +0.4 6,337,069 - Pf6N2E2_5517 0.83 -0.4 6,337,069 - Pf6N2E2_5517 0.83 -0.1 6,337,069 - Pf6N2E2_5517 0.83 +0.7 6,337,069 - Pf6N2E2_5517 0.83 -1.2 6,337,101 + Pf6N2E2_5517 0.88 -1.3 6,337,101 + Pf6N2E2_5517 0.88 +0.2 6,337,102 - Pf6N2E2_5517 0.88 -0.1 6,337,116 + +0.1 6,337,394 + Pf6N2E2_5518 0.22 -1.5
Or see this region's nucleotide sequence