Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5494 and Pf6N2E2_5495 are separated by 365 nucleotides Pf6N2E2_5495 and Pf6N2E2_5496 are separated by 290 nucleotides Pf6N2E2_5496 and Pf6N2E2_5497 are separated by 77 nucleotides
Pf6N2E2_5494 - Pyruvate kinase (EC 2.7.1.40), at 6,312,652 to 6,314,103
_5494
Pf6N2E2_5495 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 6,314,469 to 6,314,843
_5495
Pf6N2E2_5496 - FIG00953991: hypothetical protein, at 6,315,134 to 6,315,493
_5496
Pf6N2E2_5497 - hypothetical protein, at 6,315,571 to 6,315,939
_5497
Position (kb)
6314
6315 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 6313.494 kb on - strand, within Pf6N2E2_5494 at 6313.536 kb on - strand, within Pf6N2E2_5494 at 6314.055 kb on + strand at 6314.138 kb on + strand at 6314.138 kb on + strand at 6314.138 kb on + strand at 6314.139 kb on - strand at 6314.141 kb on + strand at 6314.141 kb on + strand at 6314.141 kb on + strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.142 kb on - strand at 6314.157 kb on - strand at 6314.169 kb on + strand at 6314.169 kb on + strand at 6314.170 kb on - strand at 6314.230 kb on + strand at 6314.319 kb on + strand at 6314.553 kb on + strand, within Pf6N2E2_5495 at 6314.554 kb on - strand, within Pf6N2E2_5495 at 6314.554 kb on - strand, within Pf6N2E2_5495 at 6314.554 kb on - strand, within Pf6N2E2_5495 at 6314.554 kb on - strand, within Pf6N2E2_5495 at 6314.554 kb on - strand, within Pf6N2E2_5495 at 6314.562 kb on + strand, within Pf6N2E2_5495 at 6314.562 kb on + strand, within Pf6N2E2_5495 at 6314.563 kb on - strand, within Pf6N2E2_5495 at 6314.563 kb on - strand, within Pf6N2E2_5495 at 6314.563 kb on - strand, within Pf6N2E2_5495 at 6314.635 kb on - strand, within Pf6N2E2_5495 at 6314.713 kb on - strand, within Pf6N2E2_5495 at 6314.733 kb on + strand, within Pf6N2E2_5495 at 6314.738 kb on + strand, within Pf6N2E2_5495 at 6314.906 kb on + strand at 6314.907 kb on - strand at 6314.950 kb on + strand at 6314.950 kb on + strand at 6314.950 kb on + strand at 6314.950 kb on + strand at 6314.951 kb on - strand at 6314.952 kb on + strand at 6314.952 kb on + strand at 6314.952 kb on + strand at 6314.952 kb on + strand at 6314.953 kb on - strand at 6314.953 kb on - strand at 6314.953 kb on - strand at 6314.953 kb on - strand at 6314.953 kb on - strand at 6314.972 kb on - strand at 6314.977 kb on + strand at 6314.977 kb on + strand at 6314.977 kb on + strand at 6314.978 kb on - strand at 6314.978 kb on - strand at 6314.978 kb on - strand at 6314.978 kb on - strand at 6315.017 kb on + strand at 6315.018 kb on - strand at 6315.088 kb on + strand at 6315.089 kb on - strand at 6315.089 kb on - strand at 6315.089 kb on - strand at 6315.091 kb on + strand at 6315.091 kb on + strand at 6315.091 kb on + strand at 6315.092 kb on - strand at 6315.092 kb on - strand at 6315.137 kb on + strand at 6315.137 kb on + strand at 6315.137 kb on + strand at 6315.137 kb on + strand at 6315.137 kb on + strand at 6315.137 kb on + strand at 6315.138 kb on - strand at 6315.174 kb on - strand, within Pf6N2E2_5496 at 6315.190 kb on + strand, within Pf6N2E2_5496 at 6315.264 kb on + strand, within Pf6N2E2_5496 at 6315.264 kb on + strand, within Pf6N2E2_5496 at 6315.265 kb on - strand, within Pf6N2E2_5496 at 6315.265 kb on - strand, within Pf6N2E2_5496 at 6315.458 kb on + strand at 6315.458 kb on + strand at 6315.458 kb on + strand at 6315.459 kb on - strand at 6315.561 kb on + strand at 6315.562 kb on - strand at 6315.562 kb on - strand at 6315.562 kb on - strand at 6315.572 kb on + strand at 6315.573 kb on - strand at 6315.728 kb on + strand, within Pf6N2E2_5497 at 6315.728 kb on + strand, within Pf6N2E2_5497 at 6315.728 kb on + strand, within Pf6N2E2_5497 at 6315.729 kb on - strand, within Pf6N2E2_5497 at 6315.729 kb on - strand, within Pf6N2E2_5497 at 6315.779 kb on + strand, within Pf6N2E2_5497 at 6315.779 kb on + strand, within Pf6N2E2_5497 at 6315.779 kb on + strand, within Pf6N2E2_5497 at 6315.779 kb on + strand, within Pf6N2E2_5497 at 6315.780 kb on - strand, within Pf6N2E2_5497 at 6315.833 kb on + strand, within Pf6N2E2_5497 at 6315.834 kb on - strand, within Pf6N2E2_5497 at 6315.834 kb on - strand, within Pf6N2E2_5497 at 6315.836 kb on + strand, within Pf6N2E2_5497 at 6315.836 kb on + strand, within Pf6N2E2_5497 at 6315.836 kb on + strand, within Pf6N2E2_5497 at 6315.836 kb on + strand, within Pf6N2E2_5497 at 6315.837 kb on - strand, within Pf6N2E2_5497 at 6315.837 kb on - strand, within Pf6N2E2_5497 at 6315.837 kb on - strand, within Pf6N2E2_5497 at 6315.837 kb on - strand, within Pf6N2E2_5497
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,313,494 - Pf6N2E2_5494 0.58 +5.5 6,313,536 - Pf6N2E2_5494 0.61 +4.7 6,314,055 + +6.2 6,314,138 + +4.8 6,314,138 + +6.6 6,314,138 + +4.8 6,314,139 - +4.1 6,314,141 + +6.1 6,314,141 + +5.0 6,314,141 + +6.0 6,314,142 - +4.3 6,314,142 - +4.9 6,314,142 - +4.7 6,314,142 - +6.2 6,314,142 - +4.6 6,314,142 - +4.2 6,314,142 - +3.1 6,314,157 - +3.2 6,314,169 + +5.9 6,314,169 + +6.6 6,314,170 - +4.7 6,314,230 + +6.7 6,314,319 + +5.9 6,314,553 + Pf6N2E2_5495 0.22 +2.3 6,314,554 - Pf6N2E2_5495 0.23 -0.2 6,314,554 - Pf6N2E2_5495 0.23 -0.2 6,314,554 - Pf6N2E2_5495 0.23 -0.3 6,314,554 - Pf6N2E2_5495 0.23 +0.7 6,314,554 - Pf6N2E2_5495 0.23 -2.4 6,314,562 + Pf6N2E2_5495 0.25 +2.0 6,314,562 + Pf6N2E2_5495 0.25 +1.7 6,314,563 - Pf6N2E2_5495 0.25 +0.5 6,314,563 - Pf6N2E2_5495 0.25 +0.1 6,314,563 - Pf6N2E2_5495 0.25 +0.9 6,314,635 - Pf6N2E2_5495 0.44 +0.6 6,314,713 - Pf6N2E2_5495 0.65 -0.9 6,314,733 + Pf6N2E2_5495 0.70 +1.3 6,314,738 + Pf6N2E2_5495 0.72 +2.3 6,314,906 + +0.5 6,314,907 - +0.6 6,314,950 + -0.9 6,314,950 + +1.7 6,314,950 + +0.9 6,314,950 + +0.8 6,314,951 - +0.9 6,314,952 + +3.9 6,314,952 + +0.3 6,314,952 + +1.3 6,314,952 + -0.3 6,314,953 - -0.4 6,314,953 - +0.3 6,314,953 - -0.2 6,314,953 - +0.5 6,314,953 - -2.1 6,314,972 - +1.3 6,314,977 + +1.0 6,314,977 + -0.4 6,314,977 + +0.6 6,314,978 - -0.7 6,314,978 - +0.7 6,314,978 - +1.9 6,314,978 - -1.2 6,315,017 + +1.8 6,315,018 - +0.4 6,315,088 + -0.4 6,315,089 - -0.1 6,315,089 - -0.5 6,315,089 - -0.4 6,315,091 + +0.1 6,315,091 + +1.5 6,315,091 + +0.9 6,315,092 - -0.3 6,315,092 - +0.4 6,315,137 + +0.5 6,315,137 + +0.4 6,315,137 + +1.9 6,315,137 + -0.3 6,315,137 + +0.6 6,315,137 + +1.5 6,315,138 - +1.4 6,315,174 - Pf6N2E2_5496 0.11 -1.7 6,315,190 + Pf6N2E2_5496 0.16 -0.1 6,315,264 + Pf6N2E2_5496 0.36 +1.5 6,315,264 + Pf6N2E2_5496 0.36 +1.0 6,315,265 - Pf6N2E2_5496 0.36 +0.7 6,315,265 - Pf6N2E2_5496 0.36 +0.8 6,315,458 + +0.2 6,315,458 + +1.0 6,315,458 + +1.5 6,315,459 - -0.7 6,315,561 + +1.6 6,315,562 - +0.0 6,315,562 - +0.1 6,315,562 - -0.7 6,315,572 + +0.1 6,315,573 - +1.6 6,315,728 + Pf6N2E2_5497 0.43 +1.1 6,315,728 + Pf6N2E2_5497 0.43 +1.1 6,315,728 + Pf6N2E2_5497 0.43 +1.3 6,315,729 - Pf6N2E2_5497 0.43 +2.0 6,315,729 - Pf6N2E2_5497 0.43 -2.0 6,315,779 + Pf6N2E2_5497 0.56 +1.6 6,315,779 + Pf6N2E2_5497 0.56 +0.9 6,315,779 + Pf6N2E2_5497 0.56 +1.1 6,315,779 + Pf6N2E2_5497 0.56 +0.8 6,315,780 - Pf6N2E2_5497 0.57 +0.4 6,315,833 + Pf6N2E2_5497 0.71 +0.5 6,315,834 - Pf6N2E2_5497 0.71 -1.5 6,315,834 - Pf6N2E2_5497 0.71 -0.2 6,315,836 + Pf6N2E2_5497 0.72 +0.7 6,315,836 + Pf6N2E2_5497 0.72 -0.5 6,315,836 + Pf6N2E2_5497 0.72 +1.1 6,315,836 + Pf6N2E2_5497 0.72 +0.8 6,315,837 - Pf6N2E2_5497 0.72 -0.3 6,315,837 - Pf6N2E2_5497 0.72 -0.2 6,315,837 - Pf6N2E2_5497 0.72 +0.9 6,315,837 - Pf6N2E2_5497 0.72 +0.2
Or see this region's nucleotide sequence