Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5464 and Pf6N2E2_5465 are separated by 42 nucleotides Pf6N2E2_5465 and Pf6N2E2_5466 overlap by 4 nucleotides
Pf6N2E2_5463 - sensor histidine kinase, at 6,274,973 to 6,276,775
_5463
Pf6N2E2_5464 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 6,276,772 to 6,277,731
_5464
Pf6N2E2_5465 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,277,774 to 6,279,648
_5465
Pf6N2E2_5466 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,279,645 to 6,281,936
_5466
Position (kb)
6277
6278
6279
6280 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6276.776 kb on - strand at 6276.835 kb on - strand at 6276.866 kb on + strand at 6276.866 kb on + strand at 6276.866 kb on + strand at 6276.867 kb on - strand at 6276.867 kb on - strand at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.047 kb on - strand, within Pf6N2E2_5464 at 6277.047 kb on - strand, within Pf6N2E2_5464 at 6277.121 kb on + strand, within Pf6N2E2_5464 at 6277.121 kb on + strand, within Pf6N2E2_5464 at 6277.122 kb on - strand, within Pf6N2E2_5464 at 6277.122 kb on - strand, within Pf6N2E2_5464 at 6277.187 kb on + strand, within Pf6N2E2_5464 at 6277.187 kb on + strand, within Pf6N2E2_5464 at 6277.229 kb on + strand, within Pf6N2E2_5464 at 6277.459 kb on - strand, within Pf6N2E2_5464 at 6277.723 kb on + strand at 6277.723 kb on + strand at 6277.723 kb on + strand at 6277.724 kb on - strand at 6277.724 kb on - strand at 6277.761 kb on - strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.856 kb on + strand at 6277.857 kb on - strand at 6277.857 kb on - strand at 6277.857 kb on - strand at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.061 kb on - strand, within Pf6N2E2_5465 at 6278.114 kb on - strand, within Pf6N2E2_5465 at 6278.167 kb on + strand, within Pf6N2E2_5465 at 6278.167 kb on + strand, within Pf6N2E2_5465 at 6278.236 kb on - strand, within Pf6N2E2_5465 at 6278.291 kb on + strand, within Pf6N2E2_5465 at 6278.291 kb on + strand, within Pf6N2E2_5465 at 6278.292 kb on - strand, within Pf6N2E2_5465 at 6278.501 kb on + strand, within Pf6N2E2_5465 at 6278.937 kb on + strand, within Pf6N2E2_5465 at 6279.071 kb on + strand, within Pf6N2E2_5465 at 6279.088 kb on + strand, within Pf6N2E2_5465 at 6279.089 kb on - strand, within Pf6N2E2_5465 at 6279.089 kb on - strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.405 kb on - strand, within Pf6N2E2_5465 at 6279.405 kb on - strand, within Pf6N2E2_5465 at 6279.664 kb on - strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.974 kb on - strand, within Pf6N2E2_5466 at 6279.974 kb on - strand, within Pf6N2E2_5466 at 6279.988 kb on + strand, within Pf6N2E2_5466 at 6279.988 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.154 kb on - strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.417 kb on + strand, within Pf6N2E2_5466 at 6280.418 kb on - strand, within Pf6N2E2_5466 at 6280.418 kb on - strand, within Pf6N2E2_5466 at 6280.497 kb on - strand, within Pf6N2E2_5466 at 6280.519 kb on + strand, within Pf6N2E2_5466 at 6280.607 kb on - strand, within Pf6N2E2_5466
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,276,776 - -2.6 6,276,835 - +0.7 6,276,866 + +0.7 6,276,866 + +0.2 6,276,866 + +0.6 6,276,867 - -0.2 6,276,867 - +0.5 6,277,046 + Pf6N2E2_5464 0.29 -1.1 6,277,046 + Pf6N2E2_5464 0.29 -1.4 6,277,046 + Pf6N2E2_5464 0.29 +0.7 6,277,047 - Pf6N2E2_5464 0.29 -0.1 6,277,047 - Pf6N2E2_5464 0.29 -0.6 6,277,121 + Pf6N2E2_5464 0.36 +1.1 6,277,121 + Pf6N2E2_5464 0.36 -0.1 6,277,122 - Pf6N2E2_5464 0.36 +0.0 6,277,122 - Pf6N2E2_5464 0.36 +0.6 6,277,187 + Pf6N2E2_5464 0.43 +1.7 6,277,187 + Pf6N2E2_5464 0.43 +1.1 6,277,229 + Pf6N2E2_5464 0.48 -1.9 6,277,459 - Pf6N2E2_5464 0.72 -0.0 6,277,723 + +2.3 6,277,723 + +0.8 6,277,723 + -2.0 6,277,724 - -0.0 6,277,724 - +0.8 6,277,761 - -1.4 6,277,853 + -0.6 6,277,853 + -2.5 6,277,853 + -1.3 6,277,853 + -0.7 6,277,853 + +0.2 6,277,853 + -0.0 6,277,853 + +1.8 6,277,854 - -0.4 6,277,854 - +0.5 6,277,854 - +0.1 6,277,854 - -1.2 6,277,856 + +0.4 6,277,857 - -0.2 6,277,857 - +0.0 6,277,857 - +0.1 6,278,060 + Pf6N2E2_5465 0.15 +0.3 6,278,060 + Pf6N2E2_5465 0.15 -1.0 6,278,060 + Pf6N2E2_5465 0.15 -0.5 6,278,060 + Pf6N2E2_5465 0.15 -0.6 6,278,061 - Pf6N2E2_5465 0.15 -1.5 6,278,114 - Pf6N2E2_5465 0.18 -0.4 6,278,167 + Pf6N2E2_5465 0.21 -0.6 6,278,167 + Pf6N2E2_5465 0.21 +0.1 6,278,236 - Pf6N2E2_5465 0.25 -1.6 6,278,291 + Pf6N2E2_5465 0.28 +0.9 6,278,291 + Pf6N2E2_5465 0.28 -0.6 6,278,292 - Pf6N2E2_5465 0.28 +0.4 6,278,501 + Pf6N2E2_5465 0.39 -0.4 6,278,937 + Pf6N2E2_5465 0.62 -0.6 6,279,071 + Pf6N2E2_5465 0.69 +1.5 6,279,088 + Pf6N2E2_5465 0.70 -0.2 6,279,089 - Pf6N2E2_5465 0.70 -1.4 6,279,089 - Pf6N2E2_5465 0.70 +1.3 6,279,238 + Pf6N2E2_5465 0.78 +1.4 6,279,238 + Pf6N2E2_5465 0.78 -0.6 6,279,238 + Pf6N2E2_5465 0.78 -0.6 6,279,405 - Pf6N2E2_5465 0.87 -0.4 6,279,405 - Pf6N2E2_5465 0.87 -0.5 6,279,664 - -1.4 6,279,763 + +1.1 6,279,763 + -2.0 6,279,763 + +1.4 6,279,763 + +0.2 6,279,763 + -0.5 6,279,764 - +0.3 6,279,764 - +0.5 6,279,764 - +0.5 6,279,764 - +0.8 6,279,764 - +0.7 6,279,974 - Pf6N2E2_5466 0.14 +0.2 6,279,974 - Pf6N2E2_5466 0.14 +0.7 6,279,988 + Pf6N2E2_5466 0.15 -0.1 6,279,988 + Pf6N2E2_5466 0.15 +0.7 6,280,153 + Pf6N2E2_5466 0.22 +0.7 6,280,153 + Pf6N2E2_5466 0.22 +1.3 6,280,153 + Pf6N2E2_5466 0.22 +1.2 6,280,154 - Pf6N2E2_5466 0.22 -0.2 6,280,158 + Pf6N2E2_5466 0.22 -0.1 6,280,158 + Pf6N2E2_5466 0.22 +0.1 6,280,158 + Pf6N2E2_5466 0.22 +0.3 6,280,417 + Pf6N2E2_5466 0.34 +0.2 6,280,418 - Pf6N2E2_5466 0.34 -0.0 6,280,418 - Pf6N2E2_5466 0.34 +1.3 6,280,497 - Pf6N2E2_5466 0.37 -2.7 6,280,519 + Pf6N2E2_5466 0.38 -0.9 6,280,607 - Pf6N2E2_5466 0.42 +1.0
Or see this region's nucleotide sequence