Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5428 and Pf6N2E2_5429 are separated by 49 nucleotides Pf6N2E2_5429 and Pf6N2E2_5430 are separated by 204 nucleotides Pf6N2E2_5430 and Pf6N2E2_5431 are separated by 147 nucleotides Pf6N2E2_5431 and Pf6N2E2_5432 overlap by 45 nucleotides
Pf6N2E2_5428 - DNA recombination and repair protein RecO, at 6,233,476 to 6,234,165
_5428
Pf6N2E2_5429 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 6,234,215 to 6,234,958
_5429
Pf6N2E2_5430 - Transglycosylase, Slt family, at 6,235,163 to 6,236,500
_5430
Pf6N2E2_5431 - hypothetical protein, at 6,236,648 to 6,236,956
_5431
Pf6N2E2_5432 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 6,236,912 to 6,240,808
_5432
Position (kb)
6235
6236
6237 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 6234.174 kb on + strand at 6234.174 kb on + strand at 6234.175 kb on - strand at 6234.190 kb on + strand at 6234.190 kb on + strand at 6234.190 kb on + strand at 6234.985 kb on + strand at 6234.985 kb on + strand at 6234.985 kb on + strand at 6234.986 kb on - strand at 6235.082 kb on + strand at 6235.082 kb on + strand at 6235.083 kb on - strand at 6235.083 kb on - strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.157 kb on - strand at 6235.157 kb on - strand at 6235.281 kb on + strand at 6235.354 kb on - strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.539 kb on + strand, within Pf6N2E2_5430 at 6235.539 kb on + strand, within Pf6N2E2_5430 at 6235.540 kb on - strand, within Pf6N2E2_5430 at 6235.540 kb on - strand, within Pf6N2E2_5430 at 6235.549 kb on - strand, within Pf6N2E2_5430 at 6235.738 kb on - strand, within Pf6N2E2_5430 at 6235.759 kb on - strand, within Pf6N2E2_5430 at 6235.810 kb on - strand, within Pf6N2E2_5430 at 6235.887 kb on + strand, within Pf6N2E2_5430 at 6235.887 kb on + strand, within Pf6N2E2_5430 at 6235.888 kb on - strand, within Pf6N2E2_5430 at 6235.992 kb on + strand, within Pf6N2E2_5430 at 6235.992 kb on + strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6236.063 kb on + strand, within Pf6N2E2_5430 at 6236.064 kb on - strand, within Pf6N2E2_5430 at 6236.064 kb on - strand, within Pf6N2E2_5430 at 6236.100 kb on + strand, within Pf6N2E2_5430 at 6236.101 kb on - strand, within Pf6N2E2_5430 at 6236.125 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.427 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.657 kb on + strand at 6236.657 kb on + strand at 6236.658 kb on - strand at 6236.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,234,174 + +0.5 6,234,174 + -0.7 6,234,175 - +0.6 6,234,190 + -0.7 6,234,190 + -0.9 6,234,190 + +0.8 6,234,985 + +0.2 6,234,985 + -0.2 6,234,985 + -0.1 6,234,986 - -2.4 6,235,082 + +0.4 6,235,082 + +0.2 6,235,083 - +1.4 6,235,083 - +0.7 6,235,156 + +1.3 6,235,156 + +0.7 6,235,156 + +0.7 6,235,156 + -0.2 6,235,157 - +0.3 6,235,157 - +0.9 6,235,281 + -3.3 6,235,354 - Pf6N2E2_5430 0.14 -2.1 6,235,473 + Pf6N2E2_5430 0.23 -2.6 6,235,473 + Pf6N2E2_5430 0.23 -2.9 6,235,473 + Pf6N2E2_5430 0.23 -1.8 6,235,473 + Pf6N2E2_5430 0.23 -3.0 6,235,474 - Pf6N2E2_5430 0.23 -1.7 6,235,474 - Pf6N2E2_5430 0.23 -3.6 6,235,474 - Pf6N2E2_5430 0.23 -2.7 6,235,539 + Pf6N2E2_5430 0.28 -4.1 6,235,539 + Pf6N2E2_5430 0.28 -5.0 6,235,540 - Pf6N2E2_5430 0.28 -1.0 6,235,540 - Pf6N2E2_5430 0.28 -2.3 6,235,549 - Pf6N2E2_5430 0.29 -1.7 6,235,738 - Pf6N2E2_5430 0.43 -2.8 6,235,759 - Pf6N2E2_5430 0.45 -3.0 6,235,810 - Pf6N2E2_5430 0.48 -4.4 6,235,887 + Pf6N2E2_5430 0.54 -3.1 6,235,887 + Pf6N2E2_5430 0.54 -3.1 6,235,888 - Pf6N2E2_5430 0.54 -2.7 6,235,992 + Pf6N2E2_5430 0.62 -1.6 6,235,992 + Pf6N2E2_5430 0.62 -2.7 6,235,993 - Pf6N2E2_5430 0.62 -2.0 6,235,993 - Pf6N2E2_5430 0.62 -2.5 6,235,993 - Pf6N2E2_5430 0.62 -2.7 6,235,993 - Pf6N2E2_5430 0.62 -2.0 6,235,993 - Pf6N2E2_5430 0.62 -2.8 6,236,063 + Pf6N2E2_5430 0.67 -2.7 6,236,064 - Pf6N2E2_5430 0.67 -2.1 6,236,064 - Pf6N2E2_5430 0.67 -0.5 6,236,100 + Pf6N2E2_5430 0.70 -2.0 6,236,101 - Pf6N2E2_5430 0.70 -1.7 6,236,125 + Pf6N2E2_5430 0.72 -2.6 6,236,253 + Pf6N2E2_5430 0.81 -2.8 6,236,253 + Pf6N2E2_5430 0.81 -1.8 6,236,253 + Pf6N2E2_5430 0.81 -3.6 6,236,427 + -0.7 6,236,463 + -2.5 6,236,463 + +0.2 6,236,463 + -2.0 6,236,463 + -4.2 6,236,464 - +0.3 6,236,464 - -2.4 6,236,464 - -3.2 6,236,464 - -5.0 6,236,657 + -0.6 6,236,657 + -2.2 6,236,658 - +0.1 6,236,668 + -1.8
Or see this region's nucleotide sequence