Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5424 and Pf6N2E2_5425 are separated by 31 nucleotides Pf6N2E2_5425 and Pf6N2E2_5426 overlap by 4 nucleotides Pf6N2E2_5426 and Pf6N2E2_5427 overlap by 8 nucleotides Pf6N2E2_5427 and Pf6N2E2_5428 are separated by 191 nucleotides Pf6N2E2_5428 and Pf6N2E2_5429 are separated by 49 nucleotides
Pf6N2E2_5424 - Signal peptidase I (EC 3.4.21.89), at 6,230,662 to 6,231,516
_5424
Pf6N2E2_5425 - hypothetical protein, at 6,231,548 to 6,231,703
_5425
Pf6N2E2_5426 - Ribonuclease III (EC 3.1.26.3), at 6,231,700 to 6,232,389
_5426
Pf6N2E2_5427 - GTP-binding protein Era, at 6,232,382 to 6,233,284
_5427
Pf6N2E2_5428 - DNA recombination and repair protein RecO, at 6,233,476 to 6,234,165
_5428
Pf6N2E2_5429 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 6,234,215 to 6,234,958
_5429
Position (kb)
6232
6233
6234 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6231.514 kb on + strand at 6231.514 kb on + strand at 6231.514 kb on + strand at 6231.598 kb on + strand, within Pf6N2E2_5425 at 6231.609 kb on + strand, within Pf6N2E2_5425 at 6233.282 kb on + strand at 6233.282 kb on + strand at 6233.445 kb on + strand at 6233.462 kb on + strand at 6233.462 kb on + strand at 6233.744 kb on + strand, within Pf6N2E2_5428 at 6233.744 kb on + strand, within Pf6N2E2_5428 at 6233.744 kb on + strand, within Pf6N2E2_5428 at 6233.744 kb on + strand, within Pf6N2E2_5428 at 6233.744 kb on + strand, within Pf6N2E2_5428 at 6233.745 kb on - strand, within Pf6N2E2_5428 at 6233.745 kb on - strand, within Pf6N2E2_5428 at 6233.746 kb on + strand, within Pf6N2E2_5428 at 6233.747 kb on - strand, within Pf6N2E2_5428 at 6233.747 kb on - strand, within Pf6N2E2_5428 at 6233.747 kb on - strand, within Pf6N2E2_5428 at 6233.812 kb on + strand, within Pf6N2E2_5428 at 6233.813 kb on - strand, within Pf6N2E2_5428 at 6233.903 kb on - strand, within Pf6N2E2_5428 at 6233.966 kb on + strand, within Pf6N2E2_5428 at 6233.995 kb on + strand, within Pf6N2E2_5428 at 6233.996 kb on - strand, within Pf6N2E2_5428 at 6234.174 kb on + strand at 6234.174 kb on + strand at 6234.175 kb on - strand at 6234.190 kb on + strand at 6234.190 kb on + strand at 6234.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,231,514 + +1.4 6,231,514 + +2.4 6,231,514 + +1.7 6,231,598 + Pf6N2E2_5425 0.32 +1.9 6,231,609 + Pf6N2E2_5425 0.39 -2.2 6,233,282 + -0.5 6,233,282 + -1.1 6,233,445 + +0.2 6,233,462 + +0.2 6,233,462 + +0.1 6,233,744 + Pf6N2E2_5428 0.39 -1.1 6,233,744 + Pf6N2E2_5428 0.39 -0.8 6,233,744 + Pf6N2E2_5428 0.39 +0.9 6,233,744 + Pf6N2E2_5428 0.39 -0.4 6,233,744 + Pf6N2E2_5428 0.39 -0.7 6,233,745 - Pf6N2E2_5428 0.39 -1.1 6,233,745 - Pf6N2E2_5428 0.39 -0.0 6,233,746 + Pf6N2E2_5428 0.39 -0.6 6,233,747 - Pf6N2E2_5428 0.39 -0.2 6,233,747 - Pf6N2E2_5428 0.39 +0.1 6,233,747 - Pf6N2E2_5428 0.39 +1.0 6,233,812 + Pf6N2E2_5428 0.49 +0.7 6,233,813 - Pf6N2E2_5428 0.49 -0.4 6,233,903 - Pf6N2E2_5428 0.62 +0.7 6,233,966 + Pf6N2E2_5428 0.71 -0.8 6,233,995 + Pf6N2E2_5428 0.75 -1.7 6,233,996 - Pf6N2E2_5428 0.75 +3.3 6,234,174 + +0.5 6,234,174 + -0.7 6,234,175 - +0.6 6,234,190 + -0.7 6,234,190 + -0.9 6,234,190 + +0.8
Or see this region's nucleotide sequence