Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5377 and Pf6N2E2_5378 are separated by 150 nucleotides Pf6N2E2_5378 and Pf6N2E2_5379 are separated by 14 nucleotides Pf6N2E2_5379 and Pf6N2E2_5380 are separated by 11 nucleotides
Pf6N2E2_5377 - Mannose-6-phosphate isomerase (EC 5.3.1.8) / Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 6,177,978 to 6,179,429
_5377
Pf6N2E2_5378 - alginate o-acetyltransferase AlgF, at 6,179,580 to 6,180,245
_5378
Pf6N2E2_5379 - alginate biosynthesis protein AlgJ, at 6,180,260 to 6,181,435
_5379
Pf6N2E2_5380 - Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-), at 6,181,447 to 6,183,012
_5380
Position (kb)
6180
6181
6182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6179.360 kb on + strand at 6179.403 kb on - strand at 6179.436 kb on + strand at 6179.436 kb on + strand at 6179.437 kb on - strand at 6179.460 kb on + strand at 6179.461 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.541 kb on - strand at 6179.541 kb on - strand at 6179.561 kb on + strand at 6179.561 kb on + strand at 6179.561 kb on + strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.929 kb on + strand, within Pf6N2E2_5378 at 6179.929 kb on + strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.983 kb on + strand, within Pf6N2E2_5378 at 6179.983 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.130 kb on + strand, within Pf6N2E2_5378 at 6180.230 kb on + strand at 6180.230 kb on + strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.390 kb on + strand, within Pf6N2E2_5379 at 6180.391 kb on - strand, within Pf6N2E2_5379 at 6180.391 kb on - strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.498 kb on + strand, within Pf6N2E2_5379 at 6180.499 kb on - strand, within Pf6N2E2_5379 at 6180.567 kb on + strand, within Pf6N2E2_5379 at 6180.567 kb on + strand, within Pf6N2E2_5379 at 6180.568 kb on - strand, within Pf6N2E2_5379 at 6180.973 kb on - strand, within Pf6N2E2_5379 at 6180.973 kb on - strand, within Pf6N2E2_5379 at 6181.110 kb on + strand, within Pf6N2E2_5379 at 6181.110 kb on + strand, within Pf6N2E2_5379 at 6181.110 kb on + strand, within Pf6N2E2_5379 at 6181.111 kb on - strand, within Pf6N2E2_5379 at 6181.167 kb on + strand, within Pf6N2E2_5379 at 6181.168 kb on - strand, within Pf6N2E2_5379 at 6181.168 kb on - strand, within Pf6N2E2_5379 at 6181.224 kb on + strand, within Pf6N2E2_5379 at 6181.225 kb on - strand, within Pf6N2E2_5379 at 6181.410 kb on + strand at 6181.410 kb on + strand at 6181.411 kb on - strand at 6181.421 kb on + strand at 6181.460 kb on + strand at 6181.460 kb on + strand at 6181.461 kb on - strand at 6181.461 kb on - strand at 6181.461 kb on - strand at 6181.461 kb on - strand at 6181.542 kb on - strand at 6181.565 kb on + strand at 6181.602 kb on - strand at 6181.730 kb on + strand, within Pf6N2E2_5380 at 6181.730 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.766 kb on + strand, within Pf6N2E2_5380 at 6181.767 kb on - strand, within Pf6N2E2_5380 at 6181.859 kb on + strand, within Pf6N2E2_5380 at 6181.859 kb on + strand, within Pf6N2E2_5380 at 6181.860 kb on - strand, within Pf6N2E2_5380 at 6181.860 kb on - strand, within Pf6N2E2_5380 at 6181.860 kb on - strand, within Pf6N2E2_5380 at 6182.030 kb on + strand, within Pf6N2E2_5380 at 6182.030 kb on + strand, within Pf6N2E2_5380 at 6182.030 kb on + strand, within Pf6N2E2_5380 at 6182.030 kb on + strand, within Pf6N2E2_5380 at 6182.030 kb on + strand, within Pf6N2E2_5380 at 6182.031 kb on - strand, within Pf6N2E2_5380 at 6182.031 kb on - strand, within Pf6N2E2_5380 at 6182.031 kb on - strand, within Pf6N2E2_5380 at 6182.031 kb on - strand, within Pf6N2E2_5380 at 6182.090 kb on + strand, within Pf6N2E2_5380 at 6182.091 kb on - strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.132 kb on + strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.133 kb on - strand, within Pf6N2E2_5380 at 6182.139 kb on - strand, within Pf6N2E2_5380 at 6182.139 kb on - strand, within Pf6N2E2_5380 at 6182.207 kb on + strand, within Pf6N2E2_5380 at 6182.207 kb on + strand, within Pf6N2E2_5380 at 6182.208 kb on - strand, within Pf6N2E2_5380 at 6182.208 kb on - strand, within Pf6N2E2_5380 at 6182.208 kb on - strand, within Pf6N2E2_5380 at 6182.294 kb on + strand, within Pf6N2E2_5380 at 6182.308 kb on - strand, within Pf6N2E2_5380 at 6182.309 kb on + strand, within Pf6N2E2_5380 at 6182.310 kb on - strand, within Pf6N2E2_5380 at 6182.310 kb on - strand, within Pf6N2E2_5380
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,179,360 + -0.2 6,179,403 - -0.3 6,179,436 + -0.4 6,179,436 + -1.9 6,179,437 - -2.5 6,179,460 + +0.1 6,179,461 - +0.6 6,179,467 - -0.2 6,179,467 - -0.1 6,179,467 - +0.1 6,179,467 - +2.4 6,179,467 - +0.7 6,179,541 - -1.6 6,179,541 - +1.4 6,179,561 + -3.3 6,179,561 + -2.9 6,179,561 + -0.6 6,179,582 - -1.6 6,179,582 - +1.1 6,179,582 - +0.0 6,179,582 - +0.3 6,179,644 + -1.1 6,179,644 + -0.3 6,179,644 + -1.3 6,179,644 + -1.7 6,179,644 + +0.6 6,179,926 + Pf6N2E2_5378 0.52 -0.8 6,179,926 + Pf6N2E2_5378 0.52 +0.2 6,179,926 + Pf6N2E2_5378 0.52 -0.7 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 -1.6 6,179,926 + +1.4 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 -1.3 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 +0.6 6,179,926 + Pf6N2E2_5378 0.52 -1.2 6,179,926 + Pf6N2E2_5378 0.52 -1.7 6,179,926 + Pf6N2E2_5378 0.52 -3.6 6,179,926 + Pf6N2E2_5378 0.52 -1.2 6,179,927 - Pf6N2E2_5378 0.52 +1.1 6,179,927 - Pf6N2E2_5378 0.52 -1.6 6,179,927 - Pf6N2E2_5378 0.52 -0.5 6,179,927 - Pf6N2E2_5378 0.52 -0.2 6,179,927 - Pf6N2E2_5378 0.52 -1.1 6,179,929 + Pf6N2E2_5378 0.52 -0.4 6,179,929 + Pf6N2E2_5378 0.52 -1.5 6,179,930 - Pf6N2E2_5378 0.53 -0.5 6,179,930 - Pf6N2E2_5378 0.53 -0.5 6,179,930 - Pf6N2E2_5378 0.53 -3.6 6,179,983 + Pf6N2E2_5378 0.61 -0.4 6,179,983 + Pf6N2E2_5378 0.61 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -1.5 6,180,088 + Pf6N2E2_5378 0.76 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -2.0 6,180,088 + Pf6N2E2_5378 0.76 -1.4 6,180,089 - Pf6N2E2_5378 0.76 +0.4 6,180,089 - Pf6N2E2_5378 0.76 +0.7 6,180,089 - Pf6N2E2_5378 0.76 -0.8 6,180,089 - Pf6N2E2_5378 0.76 -1.8 6,180,092 + Pf6N2E2_5378 0.77 -0.8 6,180,092 + Pf6N2E2_5378 0.77 -1.5 6,180,092 + Pf6N2E2_5378 0.77 -1.0 6,180,092 + Pf6N2E2_5378 0.77 -1.7 6,180,092 + Pf6N2E2_5378 0.77 -2.4 6,180,092 + Pf6N2E2_5378 0.77 -0.5 6,180,092 + Pf6N2E2_5378 0.77 +0.6 6,180,092 + Pf6N2E2_5378 0.77 -0.6 6,180,093 - Pf6N2E2_5378 0.77 +0.4 6,180,093 - Pf6N2E2_5378 0.77 -0.2 6,180,093 - Pf6N2E2_5378 0.77 +0.2 6,180,093 - Pf6N2E2_5378 0.77 -2.5 6,180,093 - Pf6N2E2_5378 0.77 -1.5 6,180,093 - Pf6N2E2_5378 0.77 -2.1 6,180,093 - Pf6N2E2_5378 0.77 -1.7 6,180,093 - Pf6N2E2_5378 0.77 -0.3 6,180,096 + Pf6N2E2_5378 0.77 -1.3 6,180,096 + Pf6N2E2_5378 0.77 -1.4 6,180,096 + Pf6N2E2_5378 0.77 -2.1 6,180,097 - Pf6N2E2_5378 0.78 -0.1 6,180,097 - Pf6N2E2_5378 0.78 -0.1 6,180,097 - Pf6N2E2_5378 0.78 -1.2 6,180,097 - Pf6N2E2_5378 0.78 -0.4 6,180,130 + Pf6N2E2_5378 0.83 -0.3 6,180,230 + -2.5 6,180,230 + -1.5 6,180,231 - -3.2 6,180,231 - +0.2 6,180,231 - +0.1 6,180,231 - -1.1 6,180,390 + Pf6N2E2_5379 0.11 -1.0 6,180,391 - Pf6N2E2_5379 0.11 -1.4 6,180,391 - Pf6N2E2_5379 0.11 -3.2 6,180,498 + Pf6N2E2_5379 0.20 -0.1 6,180,498 + Pf6N2E2_5379 0.20 -2.2 6,180,498 + Pf6N2E2_5379 0.20 -0.0 6,180,498 + Pf6N2E2_5379 0.20 -0.9 6,180,498 + Pf6N2E2_5379 0.20 -0.9 6,180,498 + Pf6N2E2_5379 0.20 -1.2 6,180,499 - Pf6N2E2_5379 0.20 -0.6 6,180,567 + Pf6N2E2_5379 0.26 -1.1 6,180,567 + Pf6N2E2_5379 0.26 -2.3 6,180,568 - Pf6N2E2_5379 0.26 -1.4 6,180,973 - Pf6N2E2_5379 0.61 -0.5 6,180,973 - Pf6N2E2_5379 0.61 -1.0 6,181,110 + Pf6N2E2_5379 0.72 -1.7 6,181,110 + Pf6N2E2_5379 0.72 +0.1 6,181,110 + Pf6N2E2_5379 0.72 -0.5 6,181,111 - Pf6N2E2_5379 0.72 +0.6 6,181,167 + Pf6N2E2_5379 0.77 -0.1 6,181,168 - Pf6N2E2_5379 0.77 +0.3 6,181,168 - Pf6N2E2_5379 0.77 -2.5 6,181,224 + Pf6N2E2_5379 0.82 +0.4 6,181,225 - Pf6N2E2_5379 0.82 +0.1 6,181,410 + -1.2 6,181,410 + -0.2 6,181,411 - +0.7 6,181,421 + -1.1 6,181,460 + -0.3 6,181,460 + -2.0 6,181,461 - -3.1 6,181,461 - -1.8 6,181,461 - -0.9 6,181,461 - +1.1 6,181,542 - -0.6 6,181,565 + -0.7 6,181,602 - +1.4 6,181,730 + Pf6N2E2_5380 0.18 -1.6 6,181,730 + Pf6N2E2_5380 0.18 +0.1 6,181,766 + Pf6N2E2_5380 0.20 +0.1 6,181,766 + Pf6N2E2_5380 0.20 +1.1 6,181,766 + Pf6N2E2_5380 0.20 -0.6 6,181,766 + Pf6N2E2_5380 0.20 -0.5 6,181,766 + Pf6N2E2_5380 0.20 -0.2 6,181,766 + Pf6N2E2_5380 0.20 -1.2 6,181,767 - Pf6N2E2_5380 0.20 +0.1 6,181,859 + Pf6N2E2_5380 0.26 -0.3 6,181,859 + Pf6N2E2_5380 0.26 -1.4 6,181,860 - Pf6N2E2_5380 0.26 -0.2 6,181,860 - Pf6N2E2_5380 0.26 +0.5 6,181,860 - Pf6N2E2_5380 0.26 -3.2 6,182,030 + Pf6N2E2_5380 0.37 -0.7 6,182,030 + Pf6N2E2_5380 0.37 -2.5 6,182,030 + Pf6N2E2_5380 0.37 +0.3 6,182,030 + Pf6N2E2_5380 0.37 -1.0 6,182,030 + Pf6N2E2_5380 0.37 +0.4 6,182,031 - Pf6N2E2_5380 0.37 -2.3 6,182,031 - Pf6N2E2_5380 0.37 -0.8 6,182,031 - Pf6N2E2_5380 0.37 +0.6 6,182,031 - Pf6N2E2_5380 0.37 -0.8 6,182,090 + Pf6N2E2_5380 0.41 -0.7 6,182,091 - Pf6N2E2_5380 0.41 -0.3 6,182,132 + Pf6N2E2_5380 0.44 -2.6 6,182,132 + Pf6N2E2_5380 0.44 -1.5 6,182,132 + Pf6N2E2_5380 0.44 +0.0 6,182,132 + Pf6N2E2_5380 0.44 -0.6 6,182,132 + Pf6N2E2_5380 0.44 -2.8 6,182,132 + Pf6N2E2_5380 0.44 -2.3 6,182,133 - Pf6N2E2_5380 0.44 -2.7 6,182,133 - Pf6N2E2_5380 0.44 -0.3 6,182,133 - Pf6N2E2_5380 0.44 -1.8 6,182,133 - Pf6N2E2_5380 0.44 -0.2 6,182,133 - Pf6N2E2_5380 0.44 -1.6 6,182,133 - Pf6N2E2_5380 0.44 +0.1 6,182,139 - Pf6N2E2_5380 0.44 +0.4 6,182,139 - Pf6N2E2_5380 0.44 +0.5 6,182,207 + Pf6N2E2_5380 0.49 -0.3 6,182,207 + Pf6N2E2_5380 0.49 -1.8 6,182,208 - Pf6N2E2_5380 0.49 -0.4 6,182,208 - Pf6N2E2_5380 0.49 -0.2 6,182,208 - Pf6N2E2_5380 0.49 -0.9 6,182,294 + Pf6N2E2_5380 0.54 -1.0 6,182,308 - Pf6N2E2_5380 0.55 +0.5 6,182,309 + Pf6N2E2_5380 0.55 +0.5 6,182,310 - Pf6N2E2_5380 0.55 -1.4 6,182,310 - Pf6N2E2_5380 0.55 +0.8
Or see this region's nucleotide sequence