Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5375 and Pf6N2E2_5376 are separated by 37 nucleotides Pf6N2E2_5376 and Pf6N2E2_5377 are separated by 171 nucleotides Pf6N2E2_5377 and Pf6N2E2_5378 are separated by 150 nucleotides Pf6N2E2_5378 and Pf6N2E2_5379 are separated by 14 nucleotides
Pf6N2E2_5375 - Oxidoreductase, short chain dehydrogenase/reductase family, at 6,176,480 to 6,177,304
_5375
Pf6N2E2_5376 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 6,177,342 to 6,177,806
_5376
Pf6N2E2_5377 - Mannose-6-phosphate isomerase (EC 5.3.1.8) / Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 6,177,978 to 6,179,429
_5377
Pf6N2E2_5378 - alginate o-acetyltransferase AlgF, at 6,179,580 to 6,180,245
_5378
Pf6N2E2_5379 - alginate biosynthesis protein AlgJ, at 6,180,260 to 6,181,435
_5379
Position (kb)
6177
6178
6179
6180 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6177.192 kb on - strand, within Pf6N2E2_5375 at 6177.192 kb on - strand, within Pf6N2E2_5375 at 6177.529 kb on + strand, within Pf6N2E2_5376 at 6177.530 kb on - strand, within Pf6N2E2_5376 at 6177.530 kb on - strand, within Pf6N2E2_5376 at 6177.627 kb on - strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.633 kb on + strand, within Pf6N2E2_5376 at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.980 kb on - strand at 6178.033 kb on + strand at 6178.033 kb on + strand at 6178.033 kb on + strand at 6178.034 kb on - strand at 6178.034 kb on - strand at 6178.034 kb on - strand at 6178.072 kb on + strand at 6178.072 kb on + strand at 6178.073 kb on - strand at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377 at 6178.331 kb on - strand, within Pf6N2E2_5377 at 6178.331 kb on - strand, within Pf6N2E2_5377 at 6178.331 kb on - strand, within Pf6N2E2_5377 at 6178.349 kb on - strand, within Pf6N2E2_5377 at 6178.682 kb on - strand, within Pf6N2E2_5377 at 6178.730 kb on + strand, within Pf6N2E2_5377 at 6178.731 kb on - strand, within Pf6N2E2_5377 at 6178.731 kb on - strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.783 kb on + strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.784 kb on - strand, within Pf6N2E2_5377 at 6178.861 kb on + strand, within Pf6N2E2_5377 at 6178.972 kb on + strand, within Pf6N2E2_5377 at 6178.972 kb on + strand, within Pf6N2E2_5377 at 6178.972 kb on + strand, within Pf6N2E2_5377 at 6178.972 kb on + strand, within Pf6N2E2_5377 at 6178.973 kb on - strand, within Pf6N2E2_5377 at 6179.219 kb on + strand, within Pf6N2E2_5377 at 6179.219 kb on + strand, within Pf6N2E2_5377 at 6179.219 kb on + strand, within Pf6N2E2_5377 at 6179.219 kb on + strand, within Pf6N2E2_5377 at 6179.220 kb on - strand, within Pf6N2E2_5377 at 6179.360 kb on + strand at 6179.403 kb on - strand at 6179.436 kb on + strand at 6179.436 kb on + strand at 6179.437 kb on - strand at 6179.460 kb on + strand at 6179.461 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.467 kb on - strand at 6179.541 kb on - strand at 6179.541 kb on - strand at 6179.561 kb on + strand at 6179.561 kb on + strand at 6179.561 kb on + strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.582 kb on - strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.644 kb on + strand at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.926 kb on + strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.927 kb on - strand, within Pf6N2E2_5378 at 6179.929 kb on + strand, within Pf6N2E2_5378 at 6179.929 kb on + strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.930 kb on - strand, within Pf6N2E2_5378 at 6179.983 kb on + strand, within Pf6N2E2_5378 at 6179.983 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.088 kb on + strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.089 kb on - strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.092 kb on + strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.093 kb on - strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.096 kb on + strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.097 kb on - strand, within Pf6N2E2_5378 at 6180.130 kb on + strand, within Pf6N2E2_5378 at 6180.230 kb on + strand at 6180.230 kb on + strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.231 kb on - strand at 6180.390 kb on + strand, within Pf6N2E2_5379 at 6180.391 kb on - strand, within Pf6N2E2_5379 at 6180.391 kb on - strand, within Pf6N2E2_5379
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,177,192 - Pf6N2E2_5375 0.86 +0.7 6,177,192 - Pf6N2E2_5375 0.86 +0.9 6,177,529 + Pf6N2E2_5376 0.40 +0.4 6,177,530 - Pf6N2E2_5376 0.40 +0.5 6,177,530 - Pf6N2E2_5376 0.40 +1.3 6,177,627 - Pf6N2E2_5376 0.61 +1.1 6,177,628 + Pf6N2E2_5376 0.62 +1.4 6,177,628 + Pf6N2E2_5376 0.62 +0.9 6,177,628 + Pf6N2E2_5376 0.62 +0.9 6,177,628 + Pf6N2E2_5376 0.62 +0.6 6,177,628 + Pf6N2E2_5376 0.62 -1.1 6,177,628 + Pf6N2E2_5376 0.62 -0.9 6,177,628 + Pf6N2E2_5376 0.62 -0.1 6,177,628 + Pf6N2E2_5376 0.62 -0.6 6,177,628 + Pf6N2E2_5376 0.62 +0.1 6,177,629 - Pf6N2E2_5376 0.62 -0.0 6,177,629 - Pf6N2E2_5376 0.62 -0.0 6,177,629 - Pf6N2E2_5376 0.62 -0.1 6,177,629 - Pf6N2E2_5376 0.62 +0.8 6,177,629 - Pf6N2E2_5376 0.62 +0.7 6,177,629 - Pf6N2E2_5376 0.62 -1.0 6,177,629 - Pf6N2E2_5376 0.62 +0.5 6,177,629 - Pf6N2E2_5376 0.62 +0.3 6,177,629 - Pf6N2E2_5376 0.62 -0.1 6,177,633 + Pf6N2E2_5376 0.63 -0.1 6,177,956 + -0.0 6,177,956 + -1.5 6,177,956 + +0.2 6,177,956 + +0.6 6,177,957 - -0.2 6,177,957 - +2.0 6,177,957 - -1.0 6,177,957 - -1.5 6,177,957 - +0.0 6,177,980 - +0.4 6,178,033 + -0.4 6,178,033 + -0.8 6,178,033 + +0.4 6,178,034 - -0.9 6,178,034 - -1.8 6,178,034 - -0.8 6,178,072 + -1.4 6,178,072 + +0.0 6,178,073 - -0.4 6,178,153 + Pf6N2E2_5377 0.12 -1.8 6,178,153 + Pf6N2E2_5377 0.12 -1.8 6,178,153 + Pf6N2E2_5377 0.12 +1.0 6,178,153 + Pf6N2E2_5377 0.12 -0.7 6,178,153 + Pf6N2E2_5377 0.12 -2.0 6,178,154 - Pf6N2E2_5377 0.12 +0.2 6,178,154 - Pf6N2E2_5377 0.12 -2.6 6,178,154 - Pf6N2E2_5377 0.12 -0.3 6,178,331 - Pf6N2E2_5377 0.24 -0.7 6,178,331 - Pf6N2E2_5377 0.24 +0.0 6,178,331 - Pf6N2E2_5377 0.24 +0.1 6,178,349 - Pf6N2E2_5377 0.26 -2.0 6,178,682 - Pf6N2E2_5377 0.48 -0.4 6,178,730 + Pf6N2E2_5377 0.52 -0.7 6,178,731 - Pf6N2E2_5377 0.52 -1.0 6,178,731 - Pf6N2E2_5377 0.52 -2.1 6,178,783 + Pf6N2E2_5377 0.55 -0.1 6,178,783 + Pf6N2E2_5377 0.55 -2.0 6,178,783 + Pf6N2E2_5377 0.55 -1.1 6,178,783 + Pf6N2E2_5377 0.55 -0.2 6,178,783 + Pf6N2E2_5377 0.55 -1.1 6,178,783 + Pf6N2E2_5377 0.55 -1.8 6,178,784 - Pf6N2E2_5377 0.56 -1.4 6,178,784 - Pf6N2E2_5377 0.56 -0.3 6,178,784 - Pf6N2E2_5377 0.56 -1.6 6,178,784 - Pf6N2E2_5377 0.56 -1.2 6,178,784 - Pf6N2E2_5377 0.56 -0.6 6,178,784 - Pf6N2E2_5377 0.56 +0.2 6,178,784 - Pf6N2E2_5377 0.56 -0.4 6,178,784 - Pf6N2E2_5377 0.56 -1.5 6,178,861 + Pf6N2E2_5377 0.61 -0.5 6,178,972 + Pf6N2E2_5377 0.68 +1.4 6,178,972 + Pf6N2E2_5377 0.68 -1.7 6,178,972 + Pf6N2E2_5377 0.68 -3.0 6,178,972 + Pf6N2E2_5377 0.68 -0.4 6,178,973 - Pf6N2E2_5377 0.69 -0.3 6,179,219 + Pf6N2E2_5377 0.85 +0.0 6,179,219 + Pf6N2E2_5377 0.85 -2.0 6,179,219 + Pf6N2E2_5377 0.85 -0.4 6,179,219 + Pf6N2E2_5377 0.85 -2.6 6,179,220 - Pf6N2E2_5377 0.86 -1.9 6,179,360 + -0.2 6,179,403 - -0.3 6,179,436 + -0.4 6,179,436 + -1.9 6,179,437 - -2.5 6,179,460 + +0.1 6,179,461 - +0.6 6,179,467 - -0.2 6,179,467 - -0.1 6,179,467 - +0.1 6,179,467 - +2.4 6,179,467 - +0.7 6,179,541 - -1.6 6,179,541 - +1.4 6,179,561 + -3.3 6,179,561 + -2.9 6,179,561 + -0.6 6,179,582 - -1.6 6,179,582 - +1.1 6,179,582 - +0.0 6,179,582 - +0.3 6,179,644 + -1.1 6,179,644 + -0.3 6,179,644 + -1.3 6,179,644 + -1.7 6,179,644 + +0.6 6,179,926 + Pf6N2E2_5378 0.52 -0.8 6,179,926 + Pf6N2E2_5378 0.52 +0.2 6,179,926 + Pf6N2E2_5378 0.52 -0.7 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 -1.6 6,179,926 + +1.4 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 -1.3 6,179,926 + Pf6N2E2_5378 0.52 -0.5 6,179,926 + Pf6N2E2_5378 0.52 +0.6 6,179,926 + Pf6N2E2_5378 0.52 -1.2 6,179,926 + Pf6N2E2_5378 0.52 -1.7 6,179,926 + Pf6N2E2_5378 0.52 -3.6 6,179,926 + Pf6N2E2_5378 0.52 -1.2 6,179,927 - Pf6N2E2_5378 0.52 +1.1 6,179,927 - Pf6N2E2_5378 0.52 -1.6 6,179,927 - Pf6N2E2_5378 0.52 -0.5 6,179,927 - Pf6N2E2_5378 0.52 -0.2 6,179,927 - Pf6N2E2_5378 0.52 -1.1 6,179,929 + Pf6N2E2_5378 0.52 -0.4 6,179,929 + Pf6N2E2_5378 0.52 -1.5 6,179,930 - Pf6N2E2_5378 0.53 -0.5 6,179,930 - Pf6N2E2_5378 0.53 -0.5 6,179,930 - Pf6N2E2_5378 0.53 -3.6 6,179,983 + Pf6N2E2_5378 0.61 -0.4 6,179,983 + Pf6N2E2_5378 0.61 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -1.5 6,180,088 + Pf6N2E2_5378 0.76 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -0.7 6,180,088 + Pf6N2E2_5378 0.76 -2.0 6,180,088 + Pf6N2E2_5378 0.76 -1.4 6,180,089 - Pf6N2E2_5378 0.76 +0.4 6,180,089 - Pf6N2E2_5378 0.76 +0.7 6,180,089 - Pf6N2E2_5378 0.76 -0.8 6,180,089 - Pf6N2E2_5378 0.76 -1.8 6,180,092 + Pf6N2E2_5378 0.77 -0.8 6,180,092 + Pf6N2E2_5378 0.77 -1.5 6,180,092 + Pf6N2E2_5378 0.77 -1.0 6,180,092 + Pf6N2E2_5378 0.77 -1.7 6,180,092 + Pf6N2E2_5378 0.77 -2.4 6,180,092 + Pf6N2E2_5378 0.77 -0.5 6,180,092 + Pf6N2E2_5378 0.77 +0.6 6,180,092 + Pf6N2E2_5378 0.77 -0.6 6,180,093 - Pf6N2E2_5378 0.77 +0.4 6,180,093 - Pf6N2E2_5378 0.77 -0.2 6,180,093 - Pf6N2E2_5378 0.77 +0.2 6,180,093 - Pf6N2E2_5378 0.77 -2.5 6,180,093 - Pf6N2E2_5378 0.77 -1.5 6,180,093 - Pf6N2E2_5378 0.77 -2.1 6,180,093 - Pf6N2E2_5378 0.77 -1.7 6,180,093 - Pf6N2E2_5378 0.77 -0.3 6,180,096 + Pf6N2E2_5378 0.77 -1.3 6,180,096 + Pf6N2E2_5378 0.77 -1.4 6,180,096 + Pf6N2E2_5378 0.77 -2.1 6,180,097 - Pf6N2E2_5378 0.78 -0.1 6,180,097 - Pf6N2E2_5378 0.78 -0.1 6,180,097 - Pf6N2E2_5378 0.78 -1.2 6,180,097 - Pf6N2E2_5378 0.78 -0.4 6,180,130 + Pf6N2E2_5378 0.83 -0.3 6,180,230 + -2.5 6,180,230 + -1.5 6,180,231 - -3.2 6,180,231 - +0.2 6,180,231 - +0.1 6,180,231 - -1.1 6,180,390 + Pf6N2E2_5379 0.11 -1.0 6,180,391 - Pf6N2E2_5379 0.11 -1.4 6,180,391 - Pf6N2E2_5379 0.11 -3.2
Or see this region's nucleotide sequence