Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5341 and Pf6N2E2_5342 are separated by 589 nucleotides Pf6N2E2_5342 and Pf6N2E2_5343 overlap by 4 nucleotides Pf6N2E2_5343 and Pf6N2E2_5344 are separated by 102 nucleotides
Pf6N2E2_5341 - Stringent starvation protein B, at 6,135,427 to 6,135,840
_5341
Pf6N2E2_5342 - 21 kDa hemolysin precursor, at 6,136,430 to 6,137,008
_5342
Pf6N2E2_5343 - Phosphoheptose isomerase (EC 5.3.1.-), at 6,137,005 to 6,137,598
_5343
Pf6N2E2_5344 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 6,137,701 to 6,138,063
_5344
Position (kb)
6136
6137
6138 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6135.445 kb on + strand at 6135.445 kb on + strand at 6135.445 kb on + strand at 6135.445 kb on + strand at 6135.445 kb on + strand at 6135.446 kb on - strand at 6135.463 kb on + strand at 6135.661 kb on + strand, within Pf6N2E2_5341 at 6135.661 kb on + strand, within Pf6N2E2_5341 at 6135.661 kb on + strand, within Pf6N2E2_5341 at 6135.691 kb on + strand, within Pf6N2E2_5341 at 6135.691 kb on + strand, within Pf6N2E2_5341 at 6135.691 kb on + strand, within Pf6N2E2_5341 at 6135.691 kb on + strand, within Pf6N2E2_5341 at 6135.692 kb on - strand, within Pf6N2E2_5341 at 6135.807 kb on + strand at 6135.819 kb on + strand at 6135.820 kb on - strand at 6135.825 kb on + strand at 6135.825 kb on + strand at 6135.826 kb on - strand at 6135.838 kb on + strand at 6135.838 kb on + strand at 6135.839 kb on - strand at 6135.919 kb on + strand at 6135.919 kb on + strand at 6135.919 kb on + strand at 6135.919 kb on + strand at 6135.920 kb on - strand at 6135.962 kb on - strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.965 kb on + strand at 6135.979 kb on + strand at 6135.980 kb on - strand at 6136.024 kb on + strand at 6136.231 kb on - strand at 6136.231 kb on - strand at 6136.275 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.303 kb on + strand at 6136.374 kb on + strand at 6136.375 kb on - strand at 6136.431 kb on + strand at 6136.431 kb on + strand at 6136.438 kb on + strand at 6136.438 kb on + strand at 6136.438 kb on + strand at 6136.439 kb on - strand at 6136.440 kb on + strand at 6136.440 kb on + strand at 6136.441 kb on - strand at 6136.441 kb on - strand at 6136.441 kb on - strand at 6136.441 kb on - strand at 6136.441 kb on - strand at 6136.536 kb on + strand, within Pf6N2E2_5342 at 6136.537 kb on - strand, within Pf6N2E2_5342 at 6136.682 kb on - strand, within Pf6N2E2_5342 at 6136.704 kb on - strand, within Pf6N2E2_5342 at 6136.704 kb on - strand, within Pf6N2E2_5342 at 6136.704 kb on - strand, within Pf6N2E2_5342 at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.611 kb on - strand at 6137.615 kb on - strand at 6137.814 kb on - strand, within Pf6N2E2_5344 at 6137.884 kb on - strand, within Pf6N2E2_5344 at 6137.912 kb on - strand, within Pf6N2E2_5344 at 6137.912 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.915 kb on - strand, within Pf6N2E2_5344 at 6137.920 kb on - strand, within Pf6N2E2_5344 at 6137.920 kb on - strand, within Pf6N2E2_5344
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,135,445 + +0.7 6,135,445 + +0.1 6,135,445 + -0.4 6,135,445 + +1.2 6,135,445 + -0.1 6,135,446 - -0.6 6,135,463 + -1.7 6,135,661 + Pf6N2E2_5341 0.57 +0.2 6,135,661 + Pf6N2E2_5341 0.57 -0.8 6,135,661 + Pf6N2E2_5341 0.57 +0.5 6,135,691 + Pf6N2E2_5341 0.64 -0.9 6,135,691 + Pf6N2E2_5341 0.64 +0.5 6,135,691 + Pf6N2E2_5341 0.64 +1.3 6,135,691 + Pf6N2E2_5341 0.64 +0.9 6,135,692 - Pf6N2E2_5341 0.64 -0.9 6,135,807 + +0.4 6,135,819 + -0.0 6,135,820 - +0.8 6,135,825 + -0.8 6,135,825 + -1.2 6,135,826 - +0.6 6,135,838 + +1.0 6,135,838 + +0.6 6,135,839 - +1.2 6,135,919 + -1.8 6,135,919 + -0.5 6,135,919 + -0.2 6,135,919 + -0.4 6,135,920 - +1.1 6,135,962 - -0.2 6,135,965 + +0.4 6,135,965 + -0.6 6,135,965 + +0.4 6,135,965 + +0.2 6,135,965 + +0.8 6,135,965 + +1.4 6,135,965 + -0.8 6,135,979 + +0.3 6,135,980 - +1.1 6,136,024 + +0.6 6,136,231 - -0.2 6,136,231 - -0.8 6,136,275 + -0.2 6,136,303 + +0.1 6,136,303 + +0.1 6,136,303 + -1.2 6,136,303 + +0.1 6,136,303 + -0.3 6,136,303 + +1.5 6,136,303 + +0.1 6,136,303 + +0.9 6,136,303 + +0.6 6,136,303 + +0.3 6,136,303 + -0.3 6,136,374 + +0.3 6,136,375 - -0.3 6,136,431 + +0.4 6,136,431 + -0.5 6,136,438 + -1.4 6,136,438 + +0.4 6,136,438 + -1.6 6,136,439 - -0.7 6,136,440 + +1.4 6,136,440 + -0.9 6,136,441 - -3.6 6,136,441 - -1.2 6,136,441 - -3.0 6,136,441 - -2.1 6,136,441 - -3.1 6,136,536 + Pf6N2E2_5342 0.18 -0.4 6,136,537 - Pf6N2E2_5342 0.18 -0.0 6,136,682 - Pf6N2E2_5342 0.44 -2.9 6,136,704 - Pf6N2E2_5342 0.47 -1.6 6,136,704 - Pf6N2E2_5342 0.47 -2.2 6,136,704 - Pf6N2E2_5342 0.47 -1.2 6,137,611 - +0.9 6,137,611 - -1.7 6,137,611 - -0.1 6,137,611 - +1.7 6,137,611 - -0.0 6,137,611 - +1.3 6,137,615 - +0.9 6,137,814 - Pf6N2E2_5344 0.31 +0.5 6,137,884 - Pf6N2E2_5344 0.50 +1.1 6,137,912 - Pf6N2E2_5344 0.58 -0.7 6,137,912 - Pf6N2E2_5344 0.58 +1.2 6,137,915 - Pf6N2E2_5344 0.59 +0.4 6,137,915 - Pf6N2E2_5344 0.59 -2.1 6,137,915 - Pf6N2E2_5344 0.59 +1.0 6,137,915 - Pf6N2E2_5344 0.59 -1.1 6,137,915 - Pf6N2E2_5344 0.59 +0.7 6,137,915 - Pf6N2E2_5344 0.59 +0.7 6,137,920 - Pf6N2E2_5344 0.60 -0.0 6,137,920 - Pf6N2E2_5344 0.60 +0.4
Or see this region's nucleotide sequence