Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5333 and Pf6N2E2_5334 are separated by 131 nucleotides Pf6N2E2_5334 and Pf6N2E2_5335 are separated by 245 nucleotides Pf6N2E2_5335 and Pf6N2E2_5336 are separated by 14 nucleotides
Pf6N2E2_5333 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 6,128,412 to 6,129,548
_5333
Pf6N2E2_5334 - Oxidoreductase, aldo/keto reductase family, at 6,129,680 to 6,130,720
_5334
Pf6N2E2_5335 - LSU ribosomal protein L13p (L13Ae), at 6,130,966 to 6,131,394
_5335
Pf6N2E2_5336 - SSU ribosomal protein S9p (S16e), at 6,131,409 to 6,131,801
_5336
Position (kb)
6129
6130
6131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6128.703 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.742 kb on + strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.743 kb on - strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.856 kb on + strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6128.857 kb on - strand, within Pf6N2E2_5333 at 6129.114 kb on + strand, within Pf6N2E2_5333 at 6129.131 kb on - strand, within Pf6N2E2_5333 at 6129.204 kb on + strand, within Pf6N2E2_5333 at 6129.204 kb on + strand, within Pf6N2E2_5333 at 6129.205 kb on - strand, within Pf6N2E2_5333 at 6129.205 kb on - strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.375 kb on + strand, within Pf6N2E2_5333 at 6129.376 kb on - strand, within Pf6N2E2_5333 at 6129.498 kb on + strand at 6129.499 kb on - strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.622 kb on + strand at 6129.891 kb on + strand, within Pf6N2E2_5334 at 6129.891 kb on + strand, within Pf6N2E2_5334 at 6129.892 kb on - strand, within Pf6N2E2_5334 at 6129.928 kb on - strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.945 kb on + strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.946 kb on - strand, within Pf6N2E2_5334 at 6129.988 kb on - strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.098 kb on + strand, within Pf6N2E2_5334 at 6130.335 kb on + strand, within Pf6N2E2_5334 at 6130.335 kb on + strand, within Pf6N2E2_5334 at 6130.336 kb on - strand, within Pf6N2E2_5334 at 6130.348 kb on - strand, within Pf6N2E2_5334 at 6130.348 kb on - strand, within Pf6N2E2_5334 at 6130.431 kb on + strand, within Pf6N2E2_5334 at 6130.432 kb on - strand, within Pf6N2E2_5334 at 6130.438 kb on + strand, within Pf6N2E2_5334 at 6130.506 kb on + strand, within Pf6N2E2_5334 at 6130.506 kb on + strand, within Pf6N2E2_5334 at 6130.507 kb on - strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334 at 6130.518 kb on + strand, within Pf6N2E2_5334 at 6130.563 kb on + strand, within Pf6N2E2_5334 at 6130.563 kb on + strand, within Pf6N2E2_5334 at 6130.563 kb on + strand, within Pf6N2E2_5334 at 6130.564 kb on - strand, within Pf6N2E2_5334 at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.682 kb on + strand at 6130.747 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 6,128,703 + Pf6N2E2_5333 0.26 -0.0 6,128,742 + Pf6N2E2_5333 0.29 -0.0 6,128,742 + Pf6N2E2_5333 0.29 -0.3 6,128,742 + Pf6N2E2_5333 0.29 +1.2 6,128,743 - Pf6N2E2_5333 0.29 -1.6 6,128,743 - Pf6N2E2_5333 0.29 -3.5 6,128,743 - Pf6N2E2_5333 0.29 -1.3 6,128,743 - Pf6N2E2_5333 0.29 -3.6 6,128,856 + Pf6N2E2_5333 0.39 -0.5 6,128,856 + Pf6N2E2_5333 0.39 -0.5 6,128,856 + Pf6N2E2_5333 0.39 -2.1 6,128,857 - Pf6N2E2_5333 0.39 -2.5 6,128,857 - Pf6N2E2_5333 0.39 +0.1 6,128,857 - Pf6N2E2_5333 0.39 -0.7 6,128,857 - Pf6N2E2_5333 0.39 -3.8 6,128,857 - Pf6N2E2_5333 0.39 -0.9 6,128,857 - Pf6N2E2_5333 0.39 -2.1 6,129,114 + Pf6N2E2_5333 0.62 -0.7 6,129,131 - Pf6N2E2_5333 0.63 -1.1 6,129,204 + Pf6N2E2_5333 0.70 +1.0 6,129,204 + Pf6N2E2_5333 0.70 +0.8 6,129,205 - Pf6N2E2_5333 0.70 -1.8 6,129,205 - Pf6N2E2_5333 0.70 -0.2 6,129,375 + Pf6N2E2_5333 0.85 -0.4 6,129,375 + Pf6N2E2_5333 0.85 +0.3 6,129,375 + Pf6N2E2_5333 0.85 +0.4 6,129,375 + Pf6N2E2_5333 0.85 -1.9 6,129,376 - Pf6N2E2_5333 0.85 +0.4 6,129,498 + +1.1 6,129,499 - +0.8 6,129,622 + +0.4 6,129,622 + -0.0 6,129,622 + +0.2 6,129,622 + -0.7 6,129,622 + -0.9 6,129,622 + +0.5 6,129,891 + Pf6N2E2_5334 0.20 +0.3 6,129,891 + Pf6N2E2_5334 0.20 -0.1 6,129,892 - Pf6N2E2_5334 0.20 +1.3 6,129,928 - Pf6N2E2_5334 0.24 +1.1 6,129,945 + Pf6N2E2_5334 0.25 +0.9 6,129,945 + Pf6N2E2_5334 0.25 +0.4 6,129,945 + Pf6N2E2_5334 0.25 +1.4 6,129,946 - Pf6N2E2_5334 0.26 +0.4 6,129,946 - +0.4 6,129,946 - Pf6N2E2_5334 0.26 +0.1 6,129,946 - Pf6N2E2_5334 0.26 -0.1 6,129,946 - Pf6N2E2_5334 0.26 +1.0 6,129,946 - Pf6N2E2_5334 0.26 -0.2 6,129,988 - Pf6N2E2_5334 0.30 +0.8 6,130,098 + Pf6N2E2_5334 0.40 -0.4 6,130,098 + Pf6N2E2_5334 0.40 +0.3 6,130,098 + Pf6N2E2_5334 0.40 +0.9 6,130,335 + Pf6N2E2_5334 0.63 +0.2 6,130,335 + Pf6N2E2_5334 0.63 +0.0 6,130,336 - Pf6N2E2_5334 0.63 +0.3 6,130,348 - Pf6N2E2_5334 0.64 +1.0 6,130,348 - Pf6N2E2_5334 0.64 +0.8 6,130,431 + Pf6N2E2_5334 0.72 +1.0 6,130,432 - Pf6N2E2_5334 0.72 +0.3 6,130,438 + Pf6N2E2_5334 0.73 +1.5 6,130,506 + Pf6N2E2_5334 0.79 +0.4 6,130,506 + Pf6N2E2_5334 0.79 +0.0 6,130,507 - Pf6N2E2_5334 0.79 +0.4 6,130,518 + Pf6N2E2_5334 0.80 +1.0 6,130,518 + Pf6N2E2_5334 0.80 +1.3 6,130,518 + Pf6N2E2_5334 0.80 +0.2 6,130,563 + Pf6N2E2_5334 0.85 +0.1 6,130,563 + Pf6N2E2_5334 0.85 -0.5 6,130,563 + Pf6N2E2_5334 0.85 +1.4 6,130,564 - Pf6N2E2_5334 0.85 -0.2 6,130,682 + +0.8 6,130,682 + +0.8 6,130,682 + -0.4 6,130,682 + +2.0 6,130,682 + -1.6 6,130,682 + +0.5 6,130,682 + -0.4 6,130,747 + +1.0
Or see this region's nucleotide sequence