Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5080 and Pf6N2E2_5081 are separated by 140 nucleotides Pf6N2E2_5081 and Pf6N2E2_5082 are separated by 316 nucleotides Pf6N2E2_5082 and Pf6N2E2_5083 are separated by 224 nucleotides
Pf6N2E2_5080 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 5,857,079 to 5,858,047
_5080
Pf6N2E2_5081 - hypothetical protein, at 5,858,188 to 5,858,631
_5081
Pf6N2E2_5082 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,858,948 to 5,860,201
_5082
Pf6N2E2_5083 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,860,426 to 5,864,202
_5083
Position (kb)
5858
5859
5860
5861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5858.153 kb on - strand at 5858.200 kb on + strand at 5858.201 kb on - strand at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.269 kb on + strand, within Pf6N2E2_5081 at 5858.404 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.640 kb on + strand at 5858.723 kb on + strand at 5858.752 kb on - strand at 5858.775 kb on + strand at 5858.803 kb on + strand at 5858.809 kb on + strand at 5858.893 kb on + strand at 5858.930 kb on + strand at 5860.266 kb on + strand at 5860.266 kb on + strand at 5860.267 kb on - strand at 5860.267 kb on - strand at 5860.503 kb on - strand at 5860.706 kb on + strand at 5860.707 kb on - strand at 5860.816 kb on - strand, within Pf6N2E2_5083 at 5860.872 kb on + strand, within Pf6N2E2_5083 at 5860.873 kb on - strand, within Pf6N2E2_5083 at 5861.147 kb on + strand, within Pf6N2E2_5083 at 5861.148 kb on - strand, within Pf6N2E2_5083 at 5861.150 kb on + strand, within Pf6N2E2_5083 at 5861.151 kb on - strand, within Pf6N2E2_5083
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,857,983 + +0.3 5,857,983 + +0.5 5,857,983 + -2.8 5,857,984 - -0.1 5,857,984 - -0.0 5,857,984 - -0.8 5,857,984 - -1.1 5,858,153 - -0.6 5,858,200 + -2.3 5,858,201 - -0.6 5,858,243 + Pf6N2E2_5081 0.12 -0.2 5,858,243 + Pf6N2E2_5081 0.12 +1.0 5,858,269 + Pf6N2E2_5081 0.18 -0.7 5,858,404 + Pf6N2E2_5081 0.49 +1.2 5,858,450 + Pf6N2E2_5081 0.59 -0.8 5,858,450 + Pf6N2E2_5081 0.59 -0.3 5,858,640 + +0.1 5,858,723 + -0.6 5,858,752 - +1.1 5,858,775 + -0.1 5,858,803 + +1.3 5,858,809 + -0.9 5,858,893 + -0.2 5,858,930 + +0.6 5,860,266 + -0.8 5,860,266 + -0.1 5,860,267 - +0.3 5,860,267 - +0.2 5,860,503 - -0.1 5,860,706 + -1.0 5,860,707 - -0.1 5,860,816 - Pf6N2E2_5083 0.10 -1.0 5,860,872 + Pf6N2E2_5083 0.12 -1.7 5,860,873 - Pf6N2E2_5083 0.12 -0.4 5,861,147 + Pf6N2E2_5083 0.19 -1.5 5,861,148 - Pf6N2E2_5083 0.19 +1.8 5,861,150 + Pf6N2E2_5083 0.19 -0.6 5,861,151 - Pf6N2E2_5083 0.19 -1.5
Or see this region's nucleotide sequence