Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5005 and Pf6N2E2_5006 are separated by 151 nucleotides Pf6N2E2_5006 and Pf6N2E2_5007 are separated by 112 nucleotides
Pf6N2E2_5005 - hypothetical protein, at 5,771,374 to 5,771,499
_5005
Pf6N2E2_5006 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 5,771,651 to 5,772,595
_5006
Pf6N2E2_5007 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 5,772,708 to 5,774,030
_5007
Position (kb)
5771
5772
5773 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5770.818 kb on + strand at 5770.818 kb on + strand at 5770.819 kb on - strand at 5770.881 kb on + strand at 5770.882 kb on - strand at 5770.882 kb on - strand at 5770.884 kb on - strand at 5770.884 kb on - strand at 5770.898 kb on - strand at 5770.898 kb on - strand at 5770.950 kb on + strand at 5770.950 kb on + strand at 5770.950 kb on + strand at 5770.951 kb on - strand at 5770.951 kb on - strand at 5771.048 kb on + strand at 5771.048 kb on + strand at 5771.049 kb on - strand at 5771.057 kb on - strand at 5771.089 kb on - strand at 5771.103 kb on - strand at 5771.270 kb on - strand at 5771.363 kb on + strand at 5771.547 kb on + strand at 5771.578 kb on + strand at 5771.592 kb on + strand at 5771.592 kb on + strand at 5771.592 kb on + strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.593 kb on - strand at 5771.597 kb on - strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.620 kb on + strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.621 kb on - strand at 5771.628 kb on - strand at 5771.628 kb on - strand at 5771.628 kb on - strand at 5771.628 kb on - strand at 5771.628 kb on - strand at 5771.664 kb on - strand at 5771.664 kb on - strand at 5771.670 kb on + strand at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.853 kb on + strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.996 kb on + strand, within Pf6N2E2_5006 at 5771.997 kb on - strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.177 kb on - strand, within Pf6N2E2_5006 at 5772.323 kb on + strand, within Pf6N2E2_5006 at 5772.378 kb on + strand, within Pf6N2E2_5006 at 5772.594 kb on - strand at 5772.601 kb on + strand at 5772.602 kb on - strand at 5772.602 kb on - strand at 5772.700 kb on - strand at 5772.922 kb on + strand, within Pf6N2E2_5007 at 5772.923 kb on - strand, within Pf6N2E2_5007 at 5772.958 kb on - strand, within Pf6N2E2_5007 at 5772.976 kb on + strand, within Pf6N2E2_5007 at 5772.977 kb on - strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.995 kb on - strand, within Pf6N2E2_5007 at 5773.111 kb on + strand, within Pf6N2E2_5007 at 5773.112 kb on - strand, within Pf6N2E2_5007 at 5773.177 kb on + strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,770,818 + +1.0 5,770,818 + -0.3 5,770,819 - +0.2 5,770,881 + -0.8 5,770,882 - -1.5 5,770,882 - -1.6 5,770,884 - -0.3 5,770,884 - -0.2 5,770,898 - -0.4 5,770,898 - +0.4 5,770,950 + +3.5 5,770,950 + +1.1 5,770,950 + -0.2 5,770,951 - -0.5 5,770,951 - -1.5 5,771,048 + -2.0 5,771,048 + -0.2 5,771,049 - +0.9 5,771,057 - +1.0 5,771,089 - -2.1 5,771,103 - -0.1 5,771,270 - -0.3 5,771,363 + +0.4 5,771,547 + +0.8 5,771,578 + -0.1 5,771,592 + +1.1 5,771,592 + +0.5 5,771,592 + +0.5 5,771,593 - -0.6 5,771,593 - +0.9 5,771,593 - -0.8 5,771,593 - +1.4 5,771,593 - +1.3 5,771,593 - -2.0 5,771,593 - -0.4 5,771,593 - -2.5 5,771,593 - -1.1 5,771,597 - -0.6 5,771,620 + +1.3 5,771,620 + -0.6 5,771,620 + +0.7 5,771,620 + +1.3 5,771,620 + -0.6 5,771,620 + +2.1 5,771,620 + -0.7 5,771,620 + -0.4 5,771,621 - -0.0 5,771,621 - -0.1 5,771,621 - +0.7 5,771,621 - -0.5 5,771,621 - -0.6 5,771,621 - -0.1 5,771,621 - -0.6 5,771,621 - -0.8 5,771,621 - -2.2 5,771,621 - +0.8 5,771,621 - -0.6 5,771,621 - +0.1 5,771,621 - -1.5 5,771,621 - +0.7 5,771,621 - +1.7 5,771,621 - -0.1 5,771,628 - -1.9 5,771,628 - -0.8 5,771,628 - -0.2 5,771,628 - +0.4 5,771,628 - -4.0 5,771,664 - -3.1 5,771,664 - -0.9 5,771,670 + -0.3 5,771,831 + Pf6N2E2_5006 0.19 +0.5 5,771,831 + Pf6N2E2_5006 0.19 -0.9 5,771,831 + Pf6N2E2_5006 0.19 -0.9 5,771,832 - Pf6N2E2_5006 0.19 -0.2 5,771,832 - Pf6N2E2_5006 0.19 -1.5 5,771,832 - Pf6N2E2_5006 0.19 -2.1 5,771,853 + Pf6N2E2_5006 0.21 -0.9 5,771,854 - Pf6N2E2_5006 0.21 +0.8 5,771,854 - Pf6N2E2_5006 0.21 -3.1 5,771,991 + Pf6N2E2_5006 0.36 -0.4 5,771,991 + Pf6N2E2_5006 0.36 -0.3 5,771,996 + Pf6N2E2_5006 0.37 +1.3 5,771,997 - Pf6N2E2_5006 0.37 -0.6 5,772,159 + Pf6N2E2_5006 0.54 -0.7 5,772,159 + Pf6N2E2_5006 0.54 -2.8 5,772,160 - Pf6N2E2_5006 0.54 -0.5 5,772,160 - Pf6N2E2_5006 0.54 -0.6 5,772,160 - Pf6N2E2_5006 0.54 -0.2 5,772,176 + Pf6N2E2_5006 0.56 -2.9 5,772,176 + Pf6N2E2_5006 0.56 -3.8 5,772,177 - Pf6N2E2_5006 0.56 -1.6 5,772,323 + Pf6N2E2_5006 0.71 -0.5 5,772,378 + Pf6N2E2_5006 0.77 +0.7 5,772,594 - +0.2 5,772,601 + -0.1 5,772,602 - +0.4 5,772,602 - +1.5 5,772,700 - +1.1 5,772,922 + Pf6N2E2_5007 0.16 +0.9 5,772,923 - Pf6N2E2_5007 0.16 -0.3 5,772,958 - Pf6N2E2_5007 0.19 +1.2 5,772,976 + Pf6N2E2_5007 0.20 -1.5 5,772,977 - Pf6N2E2_5007 0.20 -0.3 5,772,994 + Pf6N2E2_5007 0.22 -0.0 5,772,994 + Pf6N2E2_5007 0.22 +1.1 5,772,994 + Pf6N2E2_5007 0.22 -0.4 5,772,994 + Pf6N2E2_5007 0.22 -0.2 5,772,994 + Pf6N2E2_5007 0.22 +1.6 5,772,994 + Pf6N2E2_5007 0.22 -1.0 5,772,995 - Pf6N2E2_5007 0.22 +1.1 5,773,111 + Pf6N2E2_5007 0.30 -0.8 5,773,112 - Pf6N2E2_5007 0.31 +1.5 5,773,177 + Pf6N2E2_5007 0.35 +0.2 5,773,250 - Pf6N2E2_5007 0.41 -0.2 5,773,250 - Pf6N2E2_5007 0.41 +0.5 5,773,336 + Pf6N2E2_5007 0.47 +1.1 5,773,336 + Pf6N2E2_5007 0.47 +0.8 5,773,336 + Pf6N2E2_5007 0.47 +0.4 5,773,336 + Pf6N2E2_5007 0.47 -1.8 5,773,336 + Pf6N2E2_5007 0.47 +0.8 5,773,337 - Pf6N2E2_5007 0.48 -0.6 5,773,337 - Pf6N2E2_5007 0.48 +0.1 5,773,337 - Pf6N2E2_5007 0.48 +0.6 5,773,514 + Pf6N2E2_5007 0.61 +1.6 5,773,514 + Pf6N2E2_5007 0.61 -0.8
Or see this region's nucleotide sequence