Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4982 and Pf6N2E2_4983 are separated by 8 nucleotides Pf6N2E2_4983 and Pf6N2E2_4984 overlap by 8 nucleotides Pf6N2E2_4984 and Pf6N2E2_4985 are separated by 250 nucleotides Pf6N2E2_4985 and Pf6N2E2_4986 are separated by 27 nucleotides
Pf6N2E2_4982 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 5,749,338 to 5,750,687
_4982
Pf6N2E2_4983 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 5,750,696 to 5,751,331
_4983
Pf6N2E2_4984 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,751,324 to 5,751,608
_4984
Pf6N2E2_4985 - Nucleoside-diphosphate-sugar epimerases, at 5,751,859 to 5,752,500
_4985
Pf6N2E2_4986 - MORN repeat family protein, at 5,752,528 to 5,754,246
_4986
Position (kb)
5751
5752 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5750.579 kb on + strand at 5751.329 kb on + strand at 5751.329 kb on + strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.420 kb on + strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.606 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.722 kb on + strand at 5751.723 kb on - strand at 5751.723 kb on - strand at 5751.769 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.787 kb on - strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.857 kb on - strand at 5751.857 kb on - strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.873 kb on + strand at 5751.873 kb on + strand at 5751.874 kb on - strand at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5752.127 kb on + strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.518 kb on - strand at 5752.529 kb on + strand at 5752.529 kb on + strand at 5752.530 kb on - strand at 5752.530 kb on - strand at 5752.530 kb on - strand at 5752.578 kb on + strand at 5752.578 kb on + strand at 5752.579 kb on - strand at 5752.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,750,579 + +2.0 5,751,329 + -0.2 5,751,329 + +0.8 5,751,330 - +0.3 5,751,330 - -1.9 5,751,330 - +1.5 5,751,330 - -2.4 5,751,420 + Pf6N2E2_4984 0.34 +1.5 5,751,445 - Pf6N2E2_4984 0.42 +0.5 5,751,445 - Pf6N2E2_4984 0.42 +0.2 5,751,445 - Pf6N2E2_4984 0.42 +0.5 5,751,606 + -0.0 5,751,634 + -0.0 5,751,634 + -0.0 5,751,634 + +0.8 5,751,634 + +0.8 5,751,635 - +0.1 5,751,635 - -0.0 5,751,635 - -1.6 5,751,635 - +0.8 5,751,722 + +0.0 5,751,723 - -1.7 5,751,723 - +0.0 5,751,769 + -0.6 5,751,786 + +2.7 5,751,786 + -0.8 5,751,786 + -0.9 5,751,786 + -1.4 5,751,787 - +0.5 5,751,856 + -0.7 5,751,856 + -0.5 5,751,856 + -2.6 5,751,856 + +1.1 5,751,856 + -1.3 5,751,856 + -0.5 5,751,856 + -1.0 5,751,856 + +0.8 5,751,856 + -0.6 5,751,857 - +0.4 5,751,857 - +0.7 5,751,860 + +0.1 5,751,860 + -0.8 5,751,860 + -0.4 5,751,860 + +2.5 5,751,860 + +0.4 5,751,860 + +0.5 5,751,860 + +1.2 5,751,861 - -0.1 5,751,861 - -1.5 5,751,861 - +1.1 5,751,861 - +1.2 5,751,861 - +0.8 5,751,861 - +0.3 5,751,861 - +0.3 5,751,873 + +3.1 5,751,873 + -0.8 5,751,874 - +0.6 5,751,972 - Pf6N2E2_4985 0.18 +3.1 5,751,972 - Pf6N2E2_4985 0.18 -0.3 5,751,972 - Pf6N2E2_4985 0.18 -1.6 5,751,972 - Pf6N2E2_4985 0.18 +0.3 5,752,127 + Pf6N2E2_4985 0.42 +0.4 5,752,128 - Pf6N2E2_4985 0.42 +0.2 5,752,128 - Pf6N2E2_4985 0.42 -0.4 5,752,128 - Pf6N2E2_4985 0.42 -0.2 5,752,496 + -0.8 5,752,496 + -0.3 5,752,496 + +0.8 5,752,496 + +0.3 5,752,496 + +0.9 5,752,496 + +0.1 5,752,496 + -0.6 5,752,496 + +0.1 5,752,497 - -0.2 5,752,497 - -0.8 5,752,497 - +1.5 5,752,497 - +0.6 5,752,497 - +1.1 5,752,497 - +0.4 5,752,497 - +0.2 5,752,497 - -0.7 5,752,497 - +0.9 5,752,497 - +0.3 5,752,497 - +0.4 5,752,497 - +0.8 5,752,500 + -1.6 5,752,500 + +1.0 5,752,500 + -0.1 5,752,500 + -1.0 5,752,501 - +0.9 5,752,501 - -0.2 5,752,501 - -1.7 5,752,501 - +0.2 5,752,501 - +0.0 5,752,501 - -0.2 5,752,501 - -0.4 5,752,501 - +0.1 5,752,501 - +0.8 5,752,501 - +0.9 5,752,518 - -1.0 5,752,529 + +0.5 5,752,529 + +0.4 5,752,530 - -1.2 5,752,530 - +0.1 5,752,530 - -0.5 5,752,578 + +0.1 5,752,578 + +0.3 5,752,579 - +1.6 5,752,579 - -1.3
Or see this region's nucleotide sequence