Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4808 and Pf6N2E2_4809 are separated by 130 nucleotides Pf6N2E2_4809 and Pf6N2E2_4810 overlap by 4 nucleotides Pf6N2E2_4810 and Pf6N2E2_4811 are separated by 112 nucleotides
Pf6N2E2_4808 - Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor, at 5,557,728 to 5,560,496
_4808
Pf6N2E2_4809 - COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases, at 5,560,627 to 5,561,652
_4809
Pf6N2E2_4810 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 5,561,649 to 5,562,320
_4810
Pf6N2E2_4811 - DnaJ-like protein DjlA, at 5,562,433 to 5,563,089
_4811
Position (kb)
5560
5561
5562 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5560.582 kb on - strand at 5560.582 kb on - strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.643 kb on + strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.644 kb on - strand at 5560.769 kb on - strand, within Pf6N2E2_4809 at 5560.851 kb on + strand, within Pf6N2E2_4809 at 5560.851 kb on + strand, within Pf6N2E2_4809 at 5560.881 kb on + strand, within Pf6N2E2_4809 at 5560.954 kb on + strand, within Pf6N2E2_4809 at 5560.954 kb on + strand, within Pf6N2E2_4809 at 5560.964 kb on + strand, within Pf6N2E2_4809 at 5560.964 kb on + strand, within Pf6N2E2_4809 at 5560.964 kb on + strand, within Pf6N2E2_4809 at 5560.964 kb on + strand, within Pf6N2E2_4809 at 5560.965 kb on - strand, within Pf6N2E2_4809 at 5560.991 kb on + strand, within Pf6N2E2_4809 at 5560.991 kb on + strand at 5560.991 kb on + strand, within Pf6N2E2_4809 at 5560.992 kb on - strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.059 kb on + strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.060 kb on - strand, within Pf6N2E2_4809 at 5561.061 kb on - strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.107 kb on + strand, within Pf6N2E2_4809 at 5561.108 kb on - strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.227 kb on + strand, within Pf6N2E2_4809 at 5561.228 kb on + strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.228 kb on - strand, within Pf6N2E2_4809 at 5561.293 kb on + strand, within Pf6N2E2_4809 at 5561.294 kb on - strand, within Pf6N2E2_4809 at 5561.294 kb on - strand, within Pf6N2E2_4809 at 5561.294 kb on - strand, within Pf6N2E2_4809 at 5561.294 kb on - strand, within Pf6N2E2_4809 at 5561.308 kb on + strand, within Pf6N2E2_4809 at 5561.308 kb on + strand, within Pf6N2E2_4809 at 5561.308 kb on + strand, within Pf6N2E2_4809 at 5561.309 kb on - strand, within Pf6N2E2_4809 at 5561.309 kb on - strand, within Pf6N2E2_4809 at 5561.383 kb on + strand, within Pf6N2E2_4809 at 5561.384 kb on - strand, within Pf6N2E2_4809 at 5561.477 kb on + strand, within Pf6N2E2_4809 at 5561.477 kb on + strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.478 kb on - strand, within Pf6N2E2_4809 at 5561.530 kb on + strand, within Pf6N2E2_4809 at 5561.531 kb on - strand, within Pf6N2E2_4809 at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.560 kb on + strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.561 kb on - strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.562 kb on + strand at 5561.563 kb on - strand at 5561.563 kb on - strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.564 kb on + strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.565 kb on - strand at 5561.705 kb on + strand at 5561.706 kb on - strand at 5561.706 kb on - strand at 5561.754 kb on + strand, within Pf6N2E2_4810 at 5561.982 kb on + strand, within Pf6N2E2_4810 at 5561.983 kb on - strand, within Pf6N2E2_4810 at 5562.098 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.126 kb on + strand, within Pf6N2E2_4810 at 5562.127 kb on - strand, within Pf6N2E2_4810 at 5562.127 kb on - strand, within Pf6N2E2_4810 at 5562.127 kb on - strand, within Pf6N2E2_4810 at 5562.127 kb on - strand, within Pf6N2E2_4810 at 5562.127 kb on - strand, within Pf6N2E2_4810 at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.321 kb on + strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.322 kb on - strand at 5562.402 kb on - strand at 5562.404 kb on + strand at 5562.428 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,560,582 - -2.2 5,560,582 - -1.2 5,560,643 + -1.0 5,560,643 + -0.1 5,560,643 + -2.1 5,560,643 + -3.9 5,560,643 + -0.0 5,560,643 + +0.1 5,560,643 + -2.4 5,560,643 + -4.2 5,560,643 + -0.4 5,560,644 - -2.0 5,560,644 - -2.8 5,560,644 - -3.1 5,560,644 - -1.4 5,560,644 - -2.8 5,560,644 - -3.6 5,560,644 - -2.3 5,560,644 - -2.6 5,560,644 - -2.7 5,560,644 - +0.6 5,560,644 - -3.8 5,560,769 - Pf6N2E2_4809 0.14 -1.4 5,560,851 + Pf6N2E2_4809 0.22 -3.6 5,560,851 + Pf6N2E2_4809 0.22 -4.6 5,560,881 + Pf6N2E2_4809 0.25 -0.2 5,560,954 + Pf6N2E2_4809 0.32 -2.9 5,560,954 + Pf6N2E2_4809 0.32 -1.9 5,560,964 + Pf6N2E2_4809 0.33 -1.5 5,560,964 + Pf6N2E2_4809 0.33 -2.5 5,560,964 + Pf6N2E2_4809 0.33 -2.8 5,560,964 + Pf6N2E2_4809 0.33 -3.2 5,560,965 - Pf6N2E2_4809 0.33 -3.5 5,560,991 + Pf6N2E2_4809 0.35 -2.4 5,560,991 + +1.6 5,560,991 + Pf6N2E2_4809 0.35 -3.1 5,560,992 - Pf6N2E2_4809 0.36 -4.0 5,561,059 + Pf6N2E2_4809 0.42 -1.9 5,561,059 + Pf6N2E2_4809 0.42 -3.1 5,561,059 + Pf6N2E2_4809 0.42 -3.3 5,561,059 + Pf6N2E2_4809 0.42 -1.9 5,561,059 + Pf6N2E2_4809 0.42 -2.8 5,561,059 + Pf6N2E2_4809 0.42 -2.7 5,561,059 + Pf6N2E2_4809 0.42 -3.3 5,561,059 + Pf6N2E2_4809 0.42 -1.9 5,561,059 + Pf6N2E2_4809 0.42 -3.0 5,561,059 + Pf6N2E2_4809 0.42 -3.4 5,561,060 - Pf6N2E2_4809 0.42 -3.6 5,561,060 - Pf6N2E2_4809 0.42 -3.6 5,561,060 - Pf6N2E2_4809 0.42 +0.6 5,561,060 - Pf6N2E2_4809 0.42 -2.3 5,561,060 - Pf6N2E2_4809 0.42 +0.9 5,561,060 - Pf6N2E2_4809 0.42 -1.5 5,561,060 - Pf6N2E2_4809 0.42 -4.9 5,561,061 - Pf6N2E2_4809 0.42 +1.6 5,561,107 + Pf6N2E2_4809 0.47 -4.4 5,561,107 + Pf6N2E2_4809 0.47 -3.0 5,561,107 + Pf6N2E2_4809 0.47 -2.1 5,561,107 + Pf6N2E2_4809 0.47 -3.4 5,561,107 + Pf6N2E2_4809 0.47 -2.8 5,561,107 + Pf6N2E2_4809 0.47 -3.0 5,561,108 - Pf6N2E2_4809 0.47 -4.2 5,561,227 + Pf6N2E2_4809 0.58 -2.6 5,561,227 + Pf6N2E2_4809 0.58 -2.4 5,561,227 + Pf6N2E2_4809 0.58 -3.9 5,561,227 + Pf6N2E2_4809 0.58 -4.1 5,561,227 + Pf6N2E2_4809 0.58 -4.4 5,561,227 + Pf6N2E2_4809 0.58 -4.6 5,561,227 + Pf6N2E2_4809 0.58 -3.7 5,561,227 + Pf6N2E2_4809 0.58 -3.4 5,561,227 + Pf6N2E2_4809 0.58 -3.2 5,561,228 + Pf6N2E2_4809 0.59 -3.9 5,561,228 - Pf6N2E2_4809 0.59 -3.5 5,561,228 - Pf6N2E2_4809 0.59 -4.3 5,561,228 - Pf6N2E2_4809 0.59 -2.0 5,561,228 - Pf6N2E2_4809 0.59 -2.8 5,561,228 - Pf6N2E2_4809 0.59 -3.2 5,561,228 - Pf6N2E2_4809 0.59 -1.7 5,561,228 - Pf6N2E2_4809 0.59 -3.4 5,561,228 - Pf6N2E2_4809 0.59 -1.9 5,561,228 - Pf6N2E2_4809 0.59 -3.4 5,561,293 + Pf6N2E2_4809 0.65 -4.9 5,561,294 - Pf6N2E2_4809 0.65 -2.2 5,561,294 - Pf6N2E2_4809 0.65 -4.1 5,561,294 - Pf6N2E2_4809 0.65 -0.4 5,561,294 - Pf6N2E2_4809 0.65 -3.8 5,561,308 + Pf6N2E2_4809 0.66 -3.2 5,561,308 + Pf6N2E2_4809 0.66 -3.9 5,561,308 + Pf6N2E2_4809 0.66 -2.2 5,561,309 - Pf6N2E2_4809 0.66 -3.1 5,561,309 - Pf6N2E2_4809 0.66 -3.2 5,561,383 + Pf6N2E2_4809 0.74 -4.4 5,561,384 - Pf6N2E2_4809 0.74 -2.2 5,561,477 + Pf6N2E2_4809 0.83 -2.4 5,561,477 + Pf6N2E2_4809 0.83 -3.7 5,561,478 - Pf6N2E2_4809 0.83 -3.9 5,561,478 - Pf6N2E2_4809 0.83 -3.4 5,561,478 - Pf6N2E2_4809 0.83 -2.2 5,561,478 - Pf6N2E2_4809 0.83 -4.5 5,561,478 - Pf6N2E2_4809 0.83 -3.6 5,561,478 - Pf6N2E2_4809 0.83 -3.7 5,561,530 + Pf6N2E2_4809 0.88 -2.9 5,561,531 - Pf6N2E2_4809 0.88 -3.4 5,561,560 + -4.6 5,561,560 + -1.4 5,561,560 + -1.7 5,561,560 + -2.1 5,561,560 + -3.6 5,561,560 + -3.2 5,561,560 + -3.4 5,561,560 + -3.1 5,561,560 + -3.7 5,561,560 + -2.7 5,561,560 + -0.7 5,561,560 + -3.7 5,561,560 + -4.3 5,561,561 - -2.9 5,561,561 - -2.5 5,561,561 - -3.6 5,561,561 - -4.0 5,561,561 - -1.8 5,561,561 - -4.0 5,561,561 - -3.7 5,561,561 - -2.9 5,561,561 - -3.3 5,561,562 + -2.9 5,561,562 + -1.9 5,561,562 + -1.5 5,561,562 + -1.3 5,561,562 + -3.1 5,561,562 + -4.1 5,561,562 + -2.6 5,561,562 + -1.6 5,561,562 + -3.4 5,561,563 - -4.3 5,561,563 - -4.7 5,561,564 + -4.5 5,561,564 + -4.4 5,561,564 + -1.5 5,561,564 + -1.2 5,561,564 + -2.3 5,561,564 + -3.9 5,561,564 + -2.7 5,561,564 + -4.6 5,561,564 + -2.0 5,561,564 + -4.6 5,561,564 + -3.4 5,561,564 + -3.3 5,561,564 + -0.4 5,561,564 + -4.1 5,561,564 + -3.9 5,561,565 - -1.5 5,561,565 - -1.3 5,561,565 - -3.4 5,561,565 - -1.2 5,561,565 - -4.1 5,561,565 - -3.0 5,561,565 - -3.2 5,561,565 - -1.9 5,561,565 - -2.3 5,561,565 - -2.3 5,561,565 - -3.0 5,561,565 - -4.3 5,561,705 + -2.6 5,561,706 - -1.7 5,561,706 - -2.0 5,561,754 + Pf6N2E2_4810 0.16 -2.2 5,561,982 + Pf6N2E2_4810 0.50 -2.1 5,561,983 - Pf6N2E2_4810 0.50 -3.3 5,562,098 + Pf6N2E2_4810 0.67 -1.7 5,562,126 + Pf6N2E2_4810 0.71 -2.7 5,562,126 + Pf6N2E2_4810 0.71 -4.2 5,562,126 + Pf6N2E2_4810 0.71 -4.2 5,562,126 + Pf6N2E2_4810 0.71 -3.1 5,562,126 + Pf6N2E2_4810 0.71 -1.5 5,562,126 + Pf6N2E2_4810 0.71 -2.2 5,562,126 + Pf6N2E2_4810 0.71 -2.1 5,562,127 - Pf6N2E2_4810 0.71 -1.9 5,562,127 - Pf6N2E2_4810 0.71 -2.9 5,562,127 - Pf6N2E2_4810 0.71 -2.8 5,562,127 - Pf6N2E2_4810 0.71 -4.4 5,562,127 - Pf6N2E2_4810 0.71 -4.1 5,562,321 + -0.9 5,562,321 + -0.5 5,562,321 + +0.8 5,562,321 + +0.2 5,562,321 + +1.2 5,562,321 + +0.5 5,562,321 + +0.9 5,562,321 + +0.2 5,562,321 + +0.6 5,562,322 - +0.5 5,562,322 - +0.4 5,562,322 - +0.5 5,562,322 - -0.6 5,562,322 - -0.7 5,562,322 - -0.6 5,562,322 - +0.2 5,562,322 - +1.0 5,562,402 - -0.8 5,562,404 + -0.1 5,562,428 + -0.5
Or see this region's nucleotide sequence