Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4802 and Pf6N2E2_4803 are separated by 32 nucleotides Pf6N2E2_4803 and Pf6N2E2_4804 overlap by 1 nucleotides Pf6N2E2_4804 and Pf6N2E2_4805 are separated by 116 nucleotides Pf6N2E2_4805 and Pf6N2E2_4806 overlap by 4 nucleotides
Pf6N2E2_4802 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 5,552,896 to 5,553,225
_4802
Pf6N2E2_4803 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41), at 5,553,258 to 5,554,133
_4803
Pf6N2E2_4804 - ApaG protein, at 5,554,133 to 5,554,513
_4804
Pf6N2E2_4805 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 5,554,630 to 5,555,448
_4805
Pf6N2E2_4806 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 5,555,445 to 5,556,434
_4806
Position (kb) 5554
5555 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.595 kb on + strand, within Pf6N2E2_4803 at 5553.596 kb on - strand, within Pf6N2E2_4803 at 5553.664 kb on + strand, within Pf6N2E2_4803 at 5553.664 kb on + strand, within Pf6N2E2_4803 at 5554.124 kb on - strand at 5554.124 kb on - strand at 5554.135 kb on - strand at 5554.216 kb on - strand, within Pf6N2E2_4804 at 5554.216 kb on - strand, within Pf6N2E2_4804 at 5554.266 kb on + strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.411 kb on + strand, within Pf6N2E2_4804 at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.527 kb on + strand at 5554.528 kb on - strand at 5554.569 kb on + strand at 5554.569 kb on + strand at 5554.570 kb on - strand at 5554.588 kb on + strand at 5554.588 kb on + strand at 5554.589 kb on - strand at 5554.604 kb on - strand at 5554.961 kb on + strand, within Pf6N2E2_4805 at 5555.112 kb on - strand, within Pf6N2E2_4805 at 5555.370 kb on + strand at 5555.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,553,239 - +0.9 5,553,239 - +0.1 5,553,239 - +0.7 5,553,239 - +0.8 5,553,339 + +1.4 5,553,339 + +1.8 5,553,339 + +1.9 5,553,339 + +1.5 5,553,339 + +1.6 5,553,339 + +1.6 5,553,339 + +0.6 5,553,339 + +0.4 5,553,339 + +0.2 5,553,339 + +5.1 5,553,339 + +1.1 5,553,339 + +0.5 5,553,339 + +1.2 5,553,339 + +0.8 5,553,340 - +0.7 5,553,340 - +1.9 5,553,340 - +1.4 5,553,340 - -0.4 5,553,340 - +0.1 5,553,340 - +1.1 5,553,340 - +0.4 5,553,595 + Pf6N2E2_4803 0.38 +4.3 5,553,596 - Pf6N2E2_4803 0.39 -0.2 5,553,664 + Pf6N2E2_4803 0.46 +3.2 5,553,664 + Pf6N2E2_4803 0.46 +2.9 5,554,124 - +0.5 5,554,124 - +0.8 5,554,135 - +1.9 5,554,216 - Pf6N2E2_4804 0.22 +0.7 5,554,216 - Pf6N2E2_4804 0.22 +0.1 5,554,266 + Pf6N2E2_4804 0.35 +1.6 5,554,267 - Pf6N2E2_4804 0.35 +0.2 5,554,267 - Pf6N2E2_4804 0.35 +0.2 5,554,267 - Pf6N2E2_4804 0.35 +1.1 5,554,267 - Pf6N2E2_4804 0.35 -0.2 5,554,267 - Pf6N2E2_4804 0.35 +1.6 5,554,267 - Pf6N2E2_4804 0.35 +1.1 5,554,411 + Pf6N2E2_4804 0.73 +2.7 5,554,519 + +2.9 5,554,519 + +2.8 5,554,519 + +2.5 5,554,519 + -0.2 5,554,520 - -0.3 5,554,520 - -0.7 5,554,520 - -0.4 5,554,520 - +0.9 5,554,520 - +0.9 5,554,520 - +0.2 5,554,520 - -0.1 5,554,520 - +0.5 5,554,520 - +0.4 5,554,527 + +1.9 5,554,528 - +0.9 5,554,569 + +0.6 5,554,569 + +0.6 5,554,570 - +1.2 5,554,588 + +1.4 5,554,588 + +0.8 5,554,589 - +1.1 5,554,604 - +0.6 5,554,961 + Pf6N2E2_4805 0.40 -0.7 5,555,112 - Pf6N2E2_4805 0.59 +1.3 5,555,370 + -0.9 5,555,435 + +0.5
Or see this region's nucleotide sequence