Experiment: Phosphomycin 0.004 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_4605 and Pf6N2E2_4606 overlap by 4 nucleotides Pf6N2E2_4606 and Pf6N2E2_4607 are separated by 24 nucleotides Pf6N2E2_4607 and Pf6N2E2_4608 are separated by 85 nucleotides 
        Pf6N2E2_4605 - Dihydroorotase (EC 3.5.2.3), at 5,330,519 to 5,331,790 
        _4605 
         
        
        Pf6N2E2_4606 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 5,331,787 to 5,332,791 
        _4606 
         
        
        Pf6N2E2_4607 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 5,332,816 to 5,333,322 
        _4607 
         
        
        Pf6N2E2_4608 - Putative Holliday junction resolvase YggF, at 5,333,408 to 5,333,845 
        _4608 
         Position (kb) 5331 
5332 
5333 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.806 kb on - strand at 5332.818 kb on - strand at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.036 kb on - strand, within Pf6N2E2_4607 at 5333.136 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.232 kb on - strand, within Pf6N2E2_4607 at 5333.368 kb on - strand at 5333.374 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Phosphomycin 0.004 mg/ml remove 5,332,806 -  -1.5 5,332,806 -  -1.8 5,332,806 -  -2.0 5,332,806 -  +0.5 5,332,806 -  -1.5 5,332,806 -  -1.3 5,332,806 -  -1.9 5,332,818 -  +0.2 5,333,036 -  Pf6N2E2_4607 0.43  +1.8 5,333,036 -  Pf6N2E2_4607 0.43  +0.0 5,333,036 -  Pf6N2E2_4607 0.43  +0.8 5,333,036 -  Pf6N2E2_4607 0.43  +1.7 5,333,036 -  Pf6N2E2_4607 0.43  +0.0 5,333,136 -  Pf6N2E2_4607 0.63  +1.0 5,333,232 -  Pf6N2E2_4607 0.82  +0.8 5,333,232 -  Pf6N2E2_4607 0.82  +1.3 5,333,232 -  Pf6N2E2_4607 0.82  -1.0 5,333,368 -  +1.3 5,333,374 -  -0.1 
Or see this region's nucleotide sequence