Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4593

Experiment: Phosphomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotidesPf6N2E2_4592 and Pf6N2E2_4593 are separated by 21 nucleotidesPf6N2E2_4593 and Pf6N2E2_4594 are separated by 62 nucleotidesPf6N2E2_4594 and Pf6N2E2_4595 overlap by 4 nucleotides Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627 _4591 Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095 _4592 Pf6N2E2_4593 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 5,321,117 to 5,322,250 _4593 Pf6N2E2_4594 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 5,322,313 to 5,322,909 _4594 Pf6N2E2_4595 - FIG003551: hypothetical protein, at 5,322,906 to 5,323,340 _4595 Position (kb) 5321 5322 5323Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5320.457 kb on + strand, within Pf6N2E2_4591at 5320.457 kb on + strand, within Pf6N2E2_4591at 5320.457 kb on + strand, within Pf6N2E2_4591at 5320.457 kb on + strand, within Pf6N2E2_4591at 5320.458 kb on - strand, within Pf6N2E2_4591at 5320.458 kb on - strand, within Pf6N2E2_4591at 5320.466 kb on + strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.467 kb on - strand, within Pf6N2E2_4591at 5320.626 kb on - strandat 5320.690 kb on + strandat 5320.690 kb on + strandat 5320.691 kb on - strandat 5320.691 kb on - strandat 5320.716 kb on + strandat 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.836 kb on + strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.837 kb on - strand, within Pf6N2E2_4592at 5320.881 kb on + strand, within Pf6N2E2_4592at 5320.881 kb on + strand, within Pf6N2E2_4592at 5320.881 kb on + strand, within Pf6N2E2_4592at 5320.882 kb on - strand, within Pf6N2E2_4592at 5320.882 kb on - strand, within Pf6N2E2_4592at 5320.882 kb on - strand, within Pf6N2E2_4592at 5320.882 kb on - strand, within Pf6N2E2_4592at 5320.931 kb on + strand, within Pf6N2E2_4592at 5321.115 kb on - strandat 5321.118 kb on + strandat 5321.118 kb on + strandat 5321.118 kb on + strandat 5321.119 kb on - strandat 5321.119 kb on - strandat 5321.119 kb on - strandat 5321.119 kb on - strandat 5321.119 kb on - strandat 5321.388 kb on + strand, within Pf6N2E2_4593at 5321.388 kb on + strand, within Pf6N2E2_4593at 5321.388 kb on + strand, within Pf6N2E2_4593at 5321.389 kb on - strand, within Pf6N2E2_4593at 5321.420 kb on + strand, within Pf6N2E2_4593at 5321.421 kb on - strand, within Pf6N2E2_4593at 5321.496 kb on + strand, within Pf6N2E2_4593at 5321.496 kb on + strand, within Pf6N2E2_4593at 5321.497 kb on - strand, within Pf6N2E2_4593at 5321.506 kb on + strand, within Pf6N2E2_4593at 5321.550 kb on + strand, within Pf6N2E2_4593at 5322.141 kb on + strandat 5322.141 kb on + strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.142 kb on - strandat 5322.228 kb on + strandat 5322.228 kb on + strandat 5322.228 kb on + strandat 5322.228 kb on + strandat 5322.229 kb on - strandat 5322.229 kb on - strandat 5322.229 kb on - strandat 5322.653 kb on + strand, within Pf6N2E2_4594at 5322.654 kb on - strand, within Pf6N2E2_4594at 5322.654 kb on - strand, within Pf6N2E2_4594at 5322.654 kb on - strand, within Pf6N2E2_4594at 5322.820 kb on + strand, within Pf6N2E2_4594at 5322.886 kb on + strandat 5322.887 kb on - strandat 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.021 kb on + strand, within Pf6N2E2_4595at 5323.022 kb on - strand, within Pf6N2E2_4595at 5323.022 kb on - strand, within Pf6N2E2_4595at 5323.022 kb on - strand, within Pf6N2E2_4595at 5323.022 kb on - strand, within Pf6N2E2_4595at 5323.024 kb on + strand, within Pf6N2E2_4595at 5323.025 kb on - strand, within Pf6N2E2_4595at 5323.025 kb on - strand, within Pf6N2E2_4595at 5323.025 kb on - strand, within Pf6N2E2_4595at 5323.025 kb on - strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.228 kb on + strand, within Pf6N2E2_4595at 5323.229 kb on - strand, within Pf6N2E2_4595at 5323.229 kb on - strand, within Pf6N2E2_4595at 5323.229 kb on - strand, within Pf6N2E2_4595at 5323.229 kb on - strand, within Pf6N2E2_4595at 5323.242 kb on - strand, within Pf6N2E2_4595

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml
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5,320,457 + Pf6N2E2_4591 0.76 -1.4
5,320,457 + Pf6N2E2_4591 0.76 -0.2
5,320,457 + Pf6N2E2_4591 0.76 -0.7
5,320,457 + Pf6N2E2_4591 0.76 -0.1
5,320,458 - Pf6N2E2_4591 0.77 -1.2
5,320,458 - Pf6N2E2_4591 0.77 +0.4
5,320,466 + Pf6N2E2_4591 0.78 -0.4
5,320,467 - Pf6N2E2_4591 0.78 -0.2
5,320,467 - Pf6N2E2_4591 0.78 -1.0
5,320,467 - Pf6N2E2_4591 0.78 +0.5
5,320,467 - Pf6N2E2_4591 0.78 +2.8
5,320,467 - Pf6N2E2_4591 0.78 -0.7
5,320,467 - Pf6N2E2_4591 0.78 +0.1
5,320,467 - Pf6N2E2_4591 0.78 -1.2
5,320,626 - -3.4
5,320,690 + +1.4
5,320,690 + -0.6
5,320,691 - +0.2
5,320,691 - +1.4
5,320,716 + -1.3
5,320,836 + Pf6N2E2_4592 0.20 -0.2
5,320,836 + Pf6N2E2_4592 0.20 +1.3
5,320,836 + Pf6N2E2_4592 0.20 +0.2
5,320,836 + Pf6N2E2_4592 0.20 -0.5
5,320,836 + Pf6N2E2_4592 0.20 -1.1
5,320,836 + Pf6N2E2_4592 0.20 -0.8
5,320,837 - Pf6N2E2_4592 0.20 -1.0
5,320,837 - Pf6N2E2_4592 0.20 +0.5
5,320,837 - Pf6N2E2_4592 0.20 +0.6
5,320,837 - Pf6N2E2_4592 0.20 +0.1
5,320,837 - Pf6N2E2_4592 0.20 -0.5
5,320,837 - Pf6N2E2_4592 0.20 -0.5
5,320,837 - Pf6N2E2_4592 0.20 +1.5
5,320,837 - Pf6N2E2_4592 0.20 -0.6
5,320,881 + Pf6N2E2_4592 0.34 -0.9
5,320,881 + Pf6N2E2_4592 0.34 +0.3
5,320,881 + Pf6N2E2_4592 0.34 +1.2
5,320,882 - Pf6N2E2_4592 0.34 -0.2
5,320,882 - Pf6N2E2_4592 0.34 -0.7
5,320,882 - Pf6N2E2_4592 0.34 -0.9
5,320,882 - Pf6N2E2_4592 0.34 -0.4
5,320,931 + Pf6N2E2_4592 0.49 -0.7
5,321,115 - +1.2
5,321,118 + +0.3
5,321,118 + -2.3
5,321,118 + -0.2
5,321,119 - -0.9
5,321,119 - -0.0
5,321,119 - +0.6
5,321,119 - -0.2
5,321,119 - +0.0
5,321,388 + Pf6N2E2_4593 0.24 +0.4
5,321,388 + Pf6N2E2_4593 0.24 +1.4
5,321,388 + Pf6N2E2_4593 0.24 +1.0
5,321,389 - Pf6N2E2_4593 0.24 +1.5
5,321,420 + Pf6N2E2_4593 0.27 +3.4
5,321,421 - Pf6N2E2_4593 0.27 +0.4
5,321,496 + Pf6N2E2_4593 0.33 +1.2
5,321,496 + Pf6N2E2_4593 0.33 -0.7
5,321,497 - Pf6N2E2_4593 0.34 +1.6
5,321,506 + Pf6N2E2_4593 0.34 -1.4
5,321,550 + Pf6N2E2_4593 0.38 +2.2
5,322,141 + +0.5
5,322,141 + +0.5
5,322,142 - +2.6
5,322,142 - +1.3
5,322,142 - +0.9
5,322,142 - +0.7
5,322,142 - +0.6
5,322,142 - +0.9
5,322,142 - +1.9
5,322,228 + +0.2
5,322,228 + +1.5
5,322,228 + -0.3
5,322,228 + +2.1
5,322,229 - +1.7
5,322,229 - +1.0
5,322,229 - +0.6
5,322,653 + Pf6N2E2_4594 0.57 -0.4
5,322,654 - Pf6N2E2_4594 0.57 -2.5
5,322,654 - Pf6N2E2_4594 0.57 +1.7
5,322,654 - Pf6N2E2_4594 0.57 +0.7
5,322,820 + Pf6N2E2_4594 0.85 +1.5
5,322,886 + +1.0
5,322,887 - -1.9
5,323,021 + Pf6N2E2_4595 0.26 +0.5
5,323,021 + Pf6N2E2_4595 0.26 -0.1
5,323,021 + Pf6N2E2_4595 0.26 +0.4
5,323,021 + Pf6N2E2_4595 0.26 +1.6
5,323,021 + Pf6N2E2_4595 0.26 +0.2
5,323,021 + Pf6N2E2_4595 0.26 +1.0
5,323,022 - Pf6N2E2_4595 0.27 +0.1
5,323,022 - Pf6N2E2_4595 0.27 +1.2
5,323,022 - Pf6N2E2_4595 0.27 +0.4
5,323,022 - Pf6N2E2_4595 0.27 -1.3
5,323,024 + Pf6N2E2_4595 0.27 +0.4
5,323,025 - Pf6N2E2_4595 0.27 +0.4
5,323,025 - Pf6N2E2_4595 0.27 -0.6
5,323,025 - Pf6N2E2_4595 0.27 +1.8
5,323,025 - Pf6N2E2_4595 0.27 -1.2
5,323,228 + Pf6N2E2_4595 0.74 +1.1
5,323,228 + Pf6N2E2_4595 0.74 -0.0
5,323,228 + Pf6N2E2_4595 0.74 -0.2
5,323,228 + Pf6N2E2_4595 0.74 -0.4
5,323,228 + Pf6N2E2_4595 0.74 +1.9
5,323,228 + Pf6N2E2_4595 0.74 +0.3
5,323,229 - Pf6N2E2_4595 0.74 +0.4
5,323,229 - Pf6N2E2_4595 0.74 +0.1
5,323,229 - Pf6N2E2_4595 0.74 -0.6
5,323,229 - Pf6N2E2_4595 0.74 +1.3
5,323,242 - Pf6N2E2_4595 0.77 +1.7

Or see this region's nucleotide sequence