Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4539 and Pf6N2E2_4540 are separated by 419 nucleotides Pf6N2E2_4540 and Pf6N2E2_4541 are separated by 124 nucleotides Pf6N2E2_4541 and Pf6N2E2_4542 are separated by 206 nucleotides Pf6N2E2_4542 and Pf6N2E2_4543 are separated by 6 nucleotides Pf6N2E2_4543 and Pf6N2E2_4544 overlap by 4 nucleotides
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Pf6N2E2_4540 - hypothetical protein, at 5,273,666 to 5,273,881
_4540
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Pf6N2E2_4542 - negative regulator of hrp expression HrpV, at 5,274,662 to 5,275,039
_4542
Pf6N2E2_4543 - hypothetical protein, at 5,275,046 to 5,275,255
_4543
Pf6N2E2_4544 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 5,275,252 to 5,277,369
_4544
Position (kb) 5274
5275 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5273.113 kb on - strand, within Pf6N2E2_4539 at 5273.133 kb on + strand, within Pf6N2E2_4539 at 5273.173 kb on - strand at 5273.266 kb on + strand at 5273.394 kb on + strand at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.762 kb on - strand, within Pf6N2E2_4540 at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.898 kb on - strand at 5273.999 kb on + strand at 5273.999 kb on + strand at 5274.000 kb on - strand at 5274.362 kb on - strand, within Pf6N2E2_4541 at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.472 kb on - strand at 5274.666 kb on + strand at 5274.666 kb on + strand at 5274.667 kb on - strand at 5274.678 kb on + strand at 5274.678 kb on + strand at 5274.679 kb on - strand at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.822 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.899 kb on + strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.040 kb on - strand at 5275.040 kb on - strand at 5275.069 kb on + strand, within Pf6N2E2_4543 at 5275.070 kb on - strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,273,113 - Pf6N2E2_4539 0.88 +0.6 5,273,133 + Pf6N2E2_4539 0.90 -1.4 5,273,173 - -0.1 5,273,266 + -0.4 5,273,394 + +0.5 5,273,761 + Pf6N2E2_4540 0.44 +0.5 5,273,761 + Pf6N2E2_4540 0.44 +0.1 5,273,761 + Pf6N2E2_4540 0.44 -2.9 5,273,762 - Pf6N2E2_4540 0.44 +0.4 5,273,897 + -0.9 5,273,897 + -0.5 5,273,897 + +0.6 5,273,898 - +0.6 5,273,999 + -0.6 5,273,999 + -0.4 5,274,000 - +1.1 5,274,362 - Pf6N2E2_4541 0.79 -0.8 5,274,471 + -0.4 5,274,471 + -0.2 5,274,471 + +0.8 5,274,472 - -0.1 5,274,666 + +0.3 5,274,666 + +0.3 5,274,667 - -1.0 5,274,678 + -0.9 5,274,678 + -1.3 5,274,679 - -0.9 5,274,806 + Pf6N2E2_4542 0.38 +0.3 5,274,806 + Pf6N2E2_4542 0.38 +0.4 5,274,807 - Pf6N2E2_4542 0.38 -1.5 5,274,807 - Pf6N2E2_4542 0.38 +0.4 5,274,822 + Pf6N2E2_4542 0.42 +0.2 5,274,893 + Pf6N2E2_4542 0.61 +0.2 5,274,893 + Pf6N2E2_4542 0.61 +0.2 5,274,893 + Pf6N2E2_4542 0.61 +1.6 5,274,894 - Pf6N2E2_4542 0.61 +0.0 5,274,894 - Pf6N2E2_4542 0.61 -0.0 5,274,899 + Pf6N2E2_4542 0.63 +0.7 5,274,957 - Pf6N2E2_4542 0.78 -0.6 5,274,957 - Pf6N2E2_4542 0.78 +0.5 5,274,957 - Pf6N2E2_4542 0.78 -0.7 5,274,967 - Pf6N2E2_4542 0.81 -2.1 5,274,967 - Pf6N2E2_4542 0.81 +0.3 5,274,967 - Pf6N2E2_4542 0.81 -0.6 5,275,017 - -0.4 5,275,017 - -0.3 5,275,017 - -0.8 5,275,017 - -0.6 5,275,017 - +0.9 5,275,039 + -0.4 5,275,039 + +0.9 5,275,039 + -0.5 5,275,040 - -0.8 5,275,040 - +0.5 5,275,069 + Pf6N2E2_4543 0.11 +0.6 5,275,070 - Pf6N2E2_4543 0.11 +0.6 5,275,226 + Pf6N2E2_4543 0.86 +0.5 5,275,226 + Pf6N2E2_4543 0.86 +0.5
Or see this region's nucleotide sequence