Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4496 and Pf6N2E2_4497 are separated by 132 nucleotides Pf6N2E2_4497 and Pf6N2E2_4498 are separated by 473 nucleotides
Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989
_4496
Pf6N2E2_4497 - N-acetylglutamate synthase (EC 2.3.1.1), at 5,229,122 to 5,230,420
_4497
Pf6N2E2_4498 - Outer membrane porin, OprD family, at 5,230,894 to 5,232,216
_4498
Position (kb)
5229
5230
5231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.825 kb on + strand, within Pf6N2E2_4496 at 5228.826 kb on - strand, within Pf6N2E2_4496 at 5228.826 kb on - strand, within Pf6N2E2_4496 at 5228.900 kb on + strand at 5228.901 kb on - strand at 5228.901 kb on - strand at 5228.960 kb on + strand at 5229.100 kb on + strand at 5229.100 kb on + strand at 5229.100 kb on + strand at 5229.104 kb on + strand at 5229.107 kb on + strand at 5229.107 kb on + strand at 5229.107 kb on + strand at 5230.418 kb on + strand at 5230.419 kb on - strand at 5230.419 kb on - strand at 5230.436 kb on + strand at 5230.500 kb on - strand at 5230.537 kb on + strand at 5230.548 kb on + strand at 5230.548 kb on + strand at 5230.549 kb on - strand at 5230.566 kb on - strand at 5230.613 kb on + strand at 5230.613 kb on + strand at 5230.669 kb on + strand at 5230.669 kb on + strand at 5230.671 kb on + strand at 5230.671 kb on + strand at 5230.748 kb on + strand at 5230.749 kb on - strand at 5230.749 kb on - strand at 5230.749 kb on - strand at 5230.757 kb on + strand at 5230.758 kb on - strand at 5230.758 kb on - strand at 5230.776 kb on + strand at 5230.776 kb on + strand at 5230.776 kb on + strand at 5230.776 kb on + strand at 5230.777 kb on - strand at 5230.861 kb on + strand at 5230.861 kb on + strand at 5230.861 kb on + strand at 5230.862 kb on - strand at 5230.873 kb on - strand at 5230.965 kb on + strand at 5230.983 kb on + strand at 5230.983 kb on + strand at 5230.984 kb on - strand at 5231.005 kb on + strand at 5231.005 kb on + strand at 5231.005 kb on + strand at 5231.005 kb on + strand at 5231.006 kb on - strand at 5231.006 kb on - strand at 5231.037 kb on + strand, within Pf6N2E2_4498 at 5231.037 kb on + strand, within Pf6N2E2_4498 at 5231.037 kb on + strand, within Pf6N2E2_4498 at 5231.037 kb on + strand, within Pf6N2E2_4498 at 5231.038 kb on - strand, within Pf6N2E2_4498 at 5231.038 kb on - strand, within Pf6N2E2_4498 at 5231.195 kb on - strand, within Pf6N2E2_4498
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,228,686 + Pf6N2E2_4496 0.74 +1.8 5,228,686 + Pf6N2E2_4496 0.74 +1.8 5,228,702 + Pf6N2E2_4496 0.75 +0.9 5,228,702 + Pf6N2E2_4496 0.75 +0.4 5,228,825 + Pf6N2E2_4496 0.86 +0.5 5,228,825 + Pf6N2E2_4496 0.86 +0.6 5,228,825 + Pf6N2E2_4496 0.86 +0.0 5,228,825 + Pf6N2E2_4496 0.86 +1.2 5,228,825 + Pf6N2E2_4496 0.86 +1.5 5,228,826 - Pf6N2E2_4496 0.86 +0.5 5,228,826 - Pf6N2E2_4496 0.86 -0.3 5,228,900 + +0.7 5,228,901 - +0.8 5,228,901 - -0.1 5,228,960 + +0.1 5,229,100 + -0.3 5,229,100 + -0.4 5,229,100 + +0.7 5,229,104 + -0.3 5,229,107 + +0.8 5,229,107 + -0.4 5,229,107 + +1.7 5,230,418 + -1.0 5,230,419 - -0.3 5,230,419 - -0.0 5,230,436 + +0.6 5,230,500 - -1.4 5,230,537 + +1.3 5,230,548 + +1.6 5,230,548 + -0.2 5,230,549 - -0.1 5,230,566 - -0.3 5,230,613 + +0.6 5,230,613 + +0.7 5,230,669 + -0.1 5,230,669 + +1.7 5,230,671 + +2.7 5,230,671 + +0.3 5,230,748 + -0.3 5,230,749 - -0.1 5,230,749 - -2.4 5,230,749 - -1.9 5,230,757 + +0.4 5,230,758 - +1.6 5,230,758 - -1.6 5,230,776 + +0.6 5,230,776 + -0.0 5,230,776 + +0.4 5,230,776 + -0.2 5,230,777 - +1.8 5,230,861 + +1.1 5,230,861 + -0.8 5,230,861 + +1.1 5,230,862 - -0.4 5,230,873 - -0.4 5,230,965 + -1.1 5,230,983 + -1.5 5,230,983 + -0.9 5,230,984 - -2.5 5,231,005 + -1.2 5,231,005 + -1.5 5,231,005 + -0.1 5,231,005 + -0.3 5,231,006 - -1.5 5,231,006 - -1.0 5,231,037 + Pf6N2E2_4498 0.11 -0.4 5,231,037 + Pf6N2E2_4498 0.11 -3.2 5,231,037 + Pf6N2E2_4498 0.11 -0.9 5,231,037 + Pf6N2E2_4498 0.11 -1.9 5,231,038 - Pf6N2E2_4498 0.11 -3.2 5,231,038 - Pf6N2E2_4498 0.11 -1.4 5,231,195 - Pf6N2E2_4498 0.23 -2.2
Or see this region's nucleotide sequence