Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4486

Experiment: Phosphomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4485 and Pf6N2E2_4486 are separated by 53 nucleotidesPf6N2E2_4486 and Pf6N2E2_4487 are separated by 355 nucleotides Pf6N2E2_4485 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 5,218,266 to 5,219,348 _4485 Pf6N2E2_4486 - Glycine cleavage system H protein, at 5,219,402 to 5,219,785 _4486 Pf6N2E2_4487 - YD repeat protein, at 5,220,141 to 5,221,238 _4487 Position (kb) 5219 5220Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5218.412 kb on - strand, within Pf6N2E2_4485at 5218.412 kb on - strand, within Pf6N2E2_4485at 5218.412 kb on - strand, within Pf6N2E2_4485at 5218.518 kb on + strand, within Pf6N2E2_4485at 5218.518 kb on + strand, within Pf6N2E2_4485at 5218.519 kb on - strand, within Pf6N2E2_4485at 5218.565 kb on + strand, within Pf6N2E2_4485at 5218.565 kb on + strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.566 kb on - strand, within Pf6N2E2_4485at 5218.569 kb on + strand, within Pf6N2E2_4485at 5218.569 kb on + strand, within Pf6N2E2_4485at 5218.569 kb on + strand, within Pf6N2E2_4485at 5218.570 kb on - strand, within Pf6N2E2_4485at 5218.570 kb on - strand, within Pf6N2E2_4485at 5218.570 kb on - strand, within Pf6N2E2_4485at 5218.827 kb on + strand, within Pf6N2E2_4485at 5218.969 kb on - strand, within Pf6N2E2_4485at 5219.095 kb on + strand, within Pf6N2E2_4485at 5219.096 kb on - strand, within Pf6N2E2_4485at 5219.096 kb on - strand, within Pf6N2E2_4485at 5219.096 kb on - strand, within Pf6N2E2_4485at 5219.096 kb on - strand, within Pf6N2E2_4485at 5219.096 kb on - strand, within Pf6N2E2_4485at 5219.198 kb on + strand, within Pf6N2E2_4485at 5219.198 kb on + strand, within Pf6N2E2_4485at 5219.198 kb on + strand, within Pf6N2E2_4485at 5219.199 kb on - strand, within Pf6N2E2_4485at 5219.210 kb on + strand, within Pf6N2E2_4485at 5219.211 kb on - strand, within Pf6N2E2_4485at 5219.211 kb on - strand, within Pf6N2E2_4485at 5219.216 kb on + strand, within Pf6N2E2_4485at 5219.217 kb on - strand, within Pf6N2E2_4485at 5219.273 kb on - strandat 5219.285 kb on + strandat 5219.286 kb on - strandat 5219.346 kb on + strandat 5219.346 kb on + strandat 5219.347 kb on - strandat 5219.347 kb on - strandat 5219.347 kb on - strandat 5219.385 kb on + strandat 5219.385 kb on + strandat 5219.386 kb on - strandat 5219.386 kb on - strandat 5219.401 kb on + strandat 5219.401 kb on + strandat 5219.401 kb on + strandat 5219.401 kb on + strandat 5219.401 kb on + strandat 5219.401 kb on + strandat 5219.402 kb on - strandat 5219.402 kb on - strandat 5219.410 kb on + strandat 5219.612 kb on + strand, within Pf6N2E2_4486at 5219.612 kb on + strand, within Pf6N2E2_4486at 5219.612 kb on + strand, within Pf6N2E2_4486at 5219.612 kb on + strand, within Pf6N2E2_4486at 5219.612 kb on + strand, within Pf6N2E2_4486at 5219.613 kb on - strand, within Pf6N2E2_4486at 5219.613 kb on - strand, within Pf6N2E2_4486at 5219.613 kb on - strand, within Pf6N2E2_4486at 5219.613 kb on - strand, within Pf6N2E2_4486at 5219.613 kb on - strand, within Pf6N2E2_4486at 5219.690 kb on + strand, within Pf6N2E2_4486at 5219.740 kb on + strand, within Pf6N2E2_4486at 5219.762 kb on + strandat 5219.773 kb on + strandat 5219.905 kb on + strandat 5219.906 kb on - strandat 5219.921 kb on - strandat 5219.921 kb on - strandat 5220.032 kb on - strandat 5220.033 kb on + strandat 5220.033 kb on + strandat 5220.033 kb on + strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.034 kb on - strandat 5220.036 kb on + strandat 5220.036 kb on + strandat 5220.049 kb on - strandat 5220.068 kb on + strandat 5220.068 kb on + strandat 5220.108 kb on - strandat 5220.111 kb on + strandat 5220.112 kb on - strandat 5220.112 kb on - strandat 5220.112 kb on - strandat 5220.124 kb on + strandat 5220.124 kb on + strandat 5220.213 kb on + strandat 5220.235 kb on + strandat 5220.302 kb on + strand, within Pf6N2E2_4487at 5220.302 kb on + strand, within Pf6N2E2_4487at 5220.346 kb on + strand, within Pf6N2E2_4487at 5220.346 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.438 kb on + strand, within Pf6N2E2_4487at 5220.439 kb on - strand, within Pf6N2E2_4487at 5220.439 kb on - strand, within Pf6N2E2_4487at 5220.439 kb on - strand, within Pf6N2E2_4487at 5220.439 kb on - strand, within Pf6N2E2_4487at 5220.440 kb on + strand, within Pf6N2E2_4487at 5220.440 kb on + strand, within Pf6N2E2_4487at 5220.441 kb on - strand, within Pf6N2E2_4487at 5220.441 kb on - strand, within Pf6N2E2_4487at 5220.528 kb on + strand, within Pf6N2E2_4487at 5220.528 kb on + strand, within Pf6N2E2_4487at 5220.528 kb on + strand, within Pf6N2E2_4487at 5220.528 kb on + strand, within Pf6N2E2_4487at 5220.529 kb on - strand, within Pf6N2E2_4487at 5220.529 kb on - strand, within Pf6N2E2_4487at 5220.529 kb on - strand, within Pf6N2E2_4487at 5220.532 kb on + strand, within Pf6N2E2_4487at 5220.532 kb on + strand, within Pf6N2E2_4487at 5220.533 kb on - strand, within Pf6N2E2_4487at 5220.555 kb on + strand, within Pf6N2E2_4487at 5220.555 kb on + strand, within Pf6N2E2_4487at 5220.556 kb on - strand, within Pf6N2E2_4487at 5220.556 kb on - strand, within Pf6N2E2_4487at 5220.556 kb on - strand, within Pf6N2E2_4487at 5220.557 kb on + strand, within Pf6N2E2_4487at 5220.557 kb on + strand, within Pf6N2E2_4487at 5220.557 kb on + strand, within Pf6N2E2_4487at 5220.558 kb on - strand, within Pf6N2E2_4487at 5220.633 kb on + strand, within Pf6N2E2_4487at 5220.633 kb on + strand, within Pf6N2E2_4487at 5220.633 kb on + strand, within Pf6N2E2_4487at 5220.633 kb on + strand, within Pf6N2E2_4487at 5220.634 kb on - strand, within Pf6N2E2_4487at 5220.634 kb on - strand, within Pf6N2E2_4487at 5220.634 kb on - strand, within Pf6N2E2_4487at 5220.653 kb on + strand, within Pf6N2E2_4487at 5220.654 kb on - strand, within Pf6N2E2_4487at 5220.656 kb on + strand, within Pf6N2E2_4487at 5220.656 kb on + strand, within Pf6N2E2_4487at 5220.657 kb on - strand, within Pf6N2E2_4487at 5220.668 kb on + strand, within Pf6N2E2_4487at 5220.686 kb on - strand, within Pf6N2E2_4487at 5220.698 kb on + strand, within Pf6N2E2_4487at 5220.707 kb on + strand, within Pf6N2E2_4487at 5220.708 kb on - strand, within Pf6N2E2_4487at 5220.708 kb on - strand, within Pf6N2E2_4487at 5220.711 kb on + strand, within Pf6N2E2_4487at 5220.719 kb on + strand, within Pf6N2E2_4487at 5220.738 kb on + strand, within Pf6N2E2_4487at 5220.741 kb on + strand, within Pf6N2E2_4487at 5220.743 kb on + strand, within Pf6N2E2_4487

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml
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5,218,412 - Pf6N2E2_4485 0.13 +0.7
5,218,412 - Pf6N2E2_4485 0.13 +0.7
5,218,412 - Pf6N2E2_4485 0.13 +0.5
5,218,518 + Pf6N2E2_4485 0.23 +0.4
5,218,518 + Pf6N2E2_4485 0.23 -0.7
5,218,519 - Pf6N2E2_4485 0.23 +1.0
5,218,565 + Pf6N2E2_4485 0.28 +0.4
5,218,565 + Pf6N2E2_4485 0.28 +0.5
5,218,566 - Pf6N2E2_4485 0.28 -0.9
5,218,566 - Pf6N2E2_4485 0.28 +0.2
5,218,566 - Pf6N2E2_4485 0.28 +0.4
5,218,566 - Pf6N2E2_4485 0.28 +0.7
5,218,566 - Pf6N2E2_4485 0.28 +0.6
5,218,566 - Pf6N2E2_4485 0.28 +0.3
5,218,566 - Pf6N2E2_4485 0.28 -1.8
5,218,566 - Pf6N2E2_4485 0.28 -0.3
5,218,569 + Pf6N2E2_4485 0.28 +0.0
5,218,569 + Pf6N2E2_4485 0.28 -1.3
5,218,569 + Pf6N2E2_4485 0.28 +0.6
5,218,570 - Pf6N2E2_4485 0.28 -1.5
5,218,570 - Pf6N2E2_4485 0.28 +1.1
5,218,570 - Pf6N2E2_4485 0.28 -0.5
5,218,827 + Pf6N2E2_4485 0.52 -1.2
5,218,969 - Pf6N2E2_4485 0.65 -0.1
5,219,095 + Pf6N2E2_4485 0.77 -0.8
5,219,096 - Pf6N2E2_4485 0.77 +1.4
5,219,096 - Pf6N2E2_4485 0.77 -0.7
5,219,096 - Pf6N2E2_4485 0.77 -0.6
5,219,096 - Pf6N2E2_4485 0.77 -0.1
5,219,096 - Pf6N2E2_4485 0.77 +0.3
5,219,198 + Pf6N2E2_4485 0.86 -0.1
5,219,198 + Pf6N2E2_4485 0.86 +0.4
5,219,198 + Pf6N2E2_4485 0.86 -0.6
5,219,199 - Pf6N2E2_4485 0.86 +0.8
5,219,210 + Pf6N2E2_4485 0.87 -0.1
5,219,211 - Pf6N2E2_4485 0.87 +1.2
5,219,211 - Pf6N2E2_4485 0.87 -1.6
5,219,216 + Pf6N2E2_4485 0.88 -0.3
5,219,217 - Pf6N2E2_4485 0.88 +0.9
5,219,273 - -0.2
5,219,285 + +0.8
5,219,286 - +0.7
5,219,346 + -0.2
5,219,346 + -0.6
5,219,347 - -0.1
5,219,347 - +2.4
5,219,347 - +0.2
5,219,385 + +0.2
5,219,385 + +1.9
5,219,386 - +1.6
5,219,386 - +0.5
5,219,401 + +1.3
5,219,401 + -0.5
5,219,401 + -0.1
5,219,401 + +0.0
5,219,401 + +0.3
5,219,401 + +0.6
5,219,402 - +0.5
5,219,402 - -0.9
5,219,410 + -1.0
5,219,612 + Pf6N2E2_4486 0.55 +0.2
5,219,612 + Pf6N2E2_4486 0.55 -1.5
5,219,612 + Pf6N2E2_4486 0.55 -0.6
5,219,612 + Pf6N2E2_4486 0.55 -1.3
5,219,612 + Pf6N2E2_4486 0.55 -0.9
5,219,613 - Pf6N2E2_4486 0.55 -0.2
5,219,613 - Pf6N2E2_4486 0.55 -0.1
5,219,613 - Pf6N2E2_4486 0.55 -0.5
5,219,613 - Pf6N2E2_4486 0.55 +0.5
5,219,613 - Pf6N2E2_4486 0.55 -0.7
5,219,690 + Pf6N2E2_4486 0.75 -0.8
5,219,740 + Pf6N2E2_4486 0.88 +2.4
5,219,762 + -0.0
5,219,773 + -1.2
5,219,905 + +0.8
5,219,906 - +0.6
5,219,921 - -0.1
5,219,921 - -0.3
5,220,032 - +0.2
5,220,033 + +0.4
5,220,033 + +0.8
5,220,033 + -1.8
5,220,034 - +0.2
5,220,034 - -0.8
5,220,034 - -0.1
5,220,034 - -0.0
5,220,034 - -0.6
5,220,034 - -0.3
5,220,034 - -0.5
5,220,036 + +0.1
5,220,036 + +0.7
5,220,049 - +1.2
5,220,068 + +0.4
5,220,068 + +0.5
5,220,108 - -1.0
5,220,111 + +0.2
5,220,112 - +0.8
5,220,112 - +0.6
5,220,112 - -0.7
5,220,124 + +0.7
5,220,124 + -1.9
5,220,213 + +0.3
5,220,235 + +1.1
5,220,302 + Pf6N2E2_4487 0.15 +0.5
5,220,302 + Pf6N2E2_4487 0.15 -0.1
5,220,346 + Pf6N2E2_4487 0.19 +0.2
5,220,346 + Pf6N2E2_4487 0.19 +0.2
5,220,438 + Pf6N2E2_4487 0.27 +2.1
5,220,438 + Pf6N2E2_4487 0.27 +0.6
5,220,438 + Pf6N2E2_4487 0.27 -0.3
5,220,438 + Pf6N2E2_4487 0.27 +0.6
5,220,438 + Pf6N2E2_4487 0.27 +0.6
5,220,438 + Pf6N2E2_4487 0.27 -3.0
5,220,439 - Pf6N2E2_4487 0.27 -1.2
5,220,439 - Pf6N2E2_4487 0.27 -0.3
5,220,439 - Pf6N2E2_4487 0.27 -0.3
5,220,439 - Pf6N2E2_4487 0.27 -0.3
5,220,440 + Pf6N2E2_4487 0.27 +1.0
5,220,440 + Pf6N2E2_4487 0.27 +0.8
5,220,441 - Pf6N2E2_4487 0.27 -1.0
5,220,441 - Pf6N2E2_4487 0.27 +0.0
5,220,528 + Pf6N2E2_4487 0.35 -0.3
5,220,528 + Pf6N2E2_4487 0.35 -0.4
5,220,528 + Pf6N2E2_4487 0.35 +0.5
5,220,528 + Pf6N2E2_4487 0.35 +0.2
5,220,529 - Pf6N2E2_4487 0.35 +0.7
5,220,529 - Pf6N2E2_4487 0.35 +0.0
5,220,529 - Pf6N2E2_4487 0.35 +1.1
5,220,532 + Pf6N2E2_4487 0.36 +1.2
5,220,532 + Pf6N2E2_4487 0.36 +0.9
5,220,533 - Pf6N2E2_4487 0.36 +1.3
5,220,555 + Pf6N2E2_4487 0.38 +0.1
5,220,555 + Pf6N2E2_4487 0.38 +0.0
5,220,556 - Pf6N2E2_4487 0.38 -1.8
5,220,556 - Pf6N2E2_4487 0.38 +1.2
5,220,556 - Pf6N2E2_4487 0.38 +0.3
5,220,557 + Pf6N2E2_4487 0.38 -0.8
5,220,557 + Pf6N2E2_4487 0.38 +0.7
5,220,557 + Pf6N2E2_4487 0.38 -0.4
5,220,558 - Pf6N2E2_4487 0.38 +0.2
5,220,633 + Pf6N2E2_4487 0.45 +1.7
5,220,633 + Pf6N2E2_4487 0.45 -0.3
5,220,633 + Pf6N2E2_4487 0.45 +1.0
5,220,633 + Pf6N2E2_4487 0.45 +0.1
5,220,634 - Pf6N2E2_4487 0.45 +1.1
5,220,634 - Pf6N2E2_4487 0.45 +0.3
5,220,634 - Pf6N2E2_4487 0.45 +0.1
5,220,653 + Pf6N2E2_4487 0.47 +0.5
5,220,654 - Pf6N2E2_4487 0.47 +0.7
5,220,656 + Pf6N2E2_4487 0.47 +0.2
5,220,656 + Pf6N2E2_4487 0.47 +0.2
5,220,657 - Pf6N2E2_4487 0.47 -0.5
5,220,668 + Pf6N2E2_4487 0.48 -1.7
5,220,686 - Pf6N2E2_4487 0.50 -0.7
5,220,698 + Pf6N2E2_4487 0.51 +0.9
5,220,707 + Pf6N2E2_4487 0.52 -1.5
5,220,708 - Pf6N2E2_4487 0.52 +0.6
5,220,708 - Pf6N2E2_4487 0.52 -0.4
5,220,711 + Pf6N2E2_4487 0.52 +0.3
5,220,719 + Pf6N2E2_4487 0.53 +1.0
5,220,738 + Pf6N2E2_4487 0.54 +1.7
5,220,741 + Pf6N2E2_4487 0.55 -2.7
5,220,743 + Pf6N2E2_4487 0.55 +0.0

Or see this region's nucleotide sequence