Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4484 and Pf6N2E2_4485 are separated by 155 nucleotides Pf6N2E2_4485 and Pf6N2E2_4486 are separated by 53 nucleotides Pf6N2E2_4486 and Pf6N2E2_4487 are separated by 355 nucleotides
Pf6N2E2_4484 - Ferric iron ABC transporter, permease protein, at 5,216,494 to 5,218,110
_4484
Pf6N2E2_4485 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 5,218,266 to 5,219,348
_4485
Pf6N2E2_4486 - Glycine cleavage system H protein, at 5,219,402 to 5,219,785
_4486
Pf6N2E2_4487 - YD repeat protein, at 5,220,141 to 5,221,238
_4487
Position (kb) 5218
5219
5220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.313 kb on + strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.314 kb on - strand, within Pf6N2E2_4484 at 5217.315 kb on + strand, within Pf6N2E2_4484 at 5217.315 kb on + strand, within Pf6N2E2_4484 at 5217.315 kb on + strand, within Pf6N2E2_4484 at 5217.315 kb on + strand, within Pf6N2E2_4484 at 5217.315 kb on + strand, within Pf6N2E2_4484 at 5217.316 kb on - strand, within Pf6N2E2_4484 at 5217.316 kb on - strand, within Pf6N2E2_4484 at 5217.316 kb on - strand, within Pf6N2E2_4484 at 5217.448 kb on + strand, within Pf6N2E2_4484 at 5217.475 kb on + strand, within Pf6N2E2_4484 at 5217.475 kb on + strand, within Pf6N2E2_4484 at 5217.476 kb on - strand, within Pf6N2E2_4484 at 5217.476 kb on - strand, within Pf6N2E2_4484 at 5217.592 kb on + strand, within Pf6N2E2_4484 at 5217.593 kb on - strand, within Pf6N2E2_4484 at 5217.644 kb on - strand, within Pf6N2E2_4484 at 5217.644 kb on - strand, within Pf6N2E2_4484 at 5217.739 kb on + strand, within Pf6N2E2_4484 at 5217.766 kb on + strand, within Pf6N2E2_4484 at 5217.767 kb on - strand, within Pf6N2E2_4484 at 5217.865 kb on + strand, within Pf6N2E2_4484 at 5217.865 kb on + strand, within Pf6N2E2_4484 at 5217.865 kb on + strand, within Pf6N2E2_4484 at 5217.865 kb on + strand, within Pf6N2E2_4484 at 5217.866 kb on - strand, within Pf6N2E2_4484 at 5217.866 kb on - strand, within Pf6N2E2_4484 at 5218.049 kb on + strand at 5218.083 kb on - strand at 5218.083 kb on - strand at 5218.108 kb on + strand at 5218.108 kb on + strand at 5218.108 kb on + strand at 5218.108 kb on + strand at 5218.109 kb on - strand at 5218.122 kb on - strand at 5218.122 kb on - strand at 5218.134 kb on + strand at 5218.134 kb on + strand at 5218.134 kb on + strand at 5218.135 kb on - strand at 5218.277 kb on + strand at 5218.277 kb on + strand at 5218.277 kb on + strand at 5218.278 kb on - strand at 5218.278 kb on - strand at 5218.287 kb on + strand at 5218.287 kb on + strand at 5218.287 kb on + strand at 5218.288 kb on - strand at 5218.288 kb on - strand at 5218.288 kb on - strand at 5218.288 kb on - strand at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.518 kb on + strand, within Pf6N2E2_4485 at 5218.518 kb on + strand, within Pf6N2E2_4485 at 5218.519 kb on - strand, within Pf6N2E2_4485 at 5218.565 kb on + strand, within Pf6N2E2_4485 at 5218.565 kb on + strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.827 kb on + strand, within Pf6N2E2_4485 at 5218.969 kb on - strand, within Pf6N2E2_4485 at 5219.095 kb on + strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.199 kb on - strand, within Pf6N2E2_4485 at 5219.210 kb on + strand, within Pf6N2E2_4485 at 5219.211 kb on - strand, within Pf6N2E2_4485 at 5219.211 kb on - strand, within Pf6N2E2_4485 at 5219.216 kb on + strand, within Pf6N2E2_4485 at 5219.217 kb on - strand, within Pf6N2E2_4485 at 5219.273 kb on - strand at 5219.285 kb on + strand at 5219.286 kb on - strand at 5219.346 kb on + strand at 5219.346 kb on + strand at 5219.347 kb on - strand at 5219.347 kb on - strand at 5219.347 kb on - strand at 5219.385 kb on + strand at 5219.385 kb on + strand at 5219.386 kb on - strand at 5219.386 kb on - strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.402 kb on - strand at 5219.402 kb on - strand at 5219.410 kb on + strand at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.690 kb on + strand, within Pf6N2E2_4486 at 5219.740 kb on + strand, within Pf6N2E2_4486 at 5219.762 kb on + strand at 5219.773 kb on + strand at 5219.905 kb on + strand at 5219.906 kb on - strand at 5219.921 kb on - strand at 5219.921 kb on - strand at 5220.032 kb on - strand at 5220.033 kb on + strand at 5220.033 kb on + strand at 5220.033 kb on + strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.036 kb on + strand at 5220.036 kb on + strand at 5220.049 kb on - strand at 5220.068 kb on + strand at 5220.068 kb on + strand at 5220.108 kb on - strand at 5220.111 kb on + strand at 5220.112 kb on - strand at 5220.112 kb on - strand at 5220.112 kb on - strand at 5220.124 kb on + strand at 5220.124 kb on + strand at 5220.213 kb on + strand at 5220.235 kb on + strand at 5220.302 kb on + strand, within Pf6N2E2_4487 at 5220.302 kb on + strand, within Pf6N2E2_4487 at 5220.346 kb on + strand, within Pf6N2E2_4487 at 5220.346 kb on + strand, within Pf6N2E2_4487
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,217,313 + Pf6N2E2_4484 0.51 +0.1 5,217,313 + Pf6N2E2_4484 0.51 +0.3 5,217,313 + Pf6N2E2_4484 0.51 +0.0 5,217,313 + Pf6N2E2_4484 0.51 +0.7 5,217,313 + Pf6N2E2_4484 0.51 +1.0 5,217,313 + Pf6N2E2_4484 0.51 -0.5 5,217,313 + Pf6N2E2_4484 0.51 +0.5 5,217,314 - Pf6N2E2_4484 0.51 +0.3 5,217,314 - Pf6N2E2_4484 0.51 -0.1 5,217,314 - Pf6N2E2_4484 0.51 -0.1 5,217,314 - Pf6N2E2_4484 0.51 +0.3 5,217,314 - Pf6N2E2_4484 0.51 -3.6 5,217,314 - Pf6N2E2_4484 0.51 +0.4 5,217,315 + Pf6N2E2_4484 0.51 -0.5 5,217,315 + Pf6N2E2_4484 0.51 -1.5 5,217,315 + Pf6N2E2_4484 0.51 +0.0 5,217,315 + Pf6N2E2_4484 0.51 -0.5 5,217,315 + Pf6N2E2_4484 0.51 +0.2 5,217,316 - Pf6N2E2_4484 0.51 +1.1 5,217,316 - Pf6N2E2_4484 0.51 +0.8 5,217,316 - Pf6N2E2_4484 0.51 -0.1 5,217,448 + Pf6N2E2_4484 0.59 +0.2 5,217,475 + Pf6N2E2_4484 0.61 +0.7 5,217,475 + Pf6N2E2_4484 0.61 -0.0 5,217,476 - Pf6N2E2_4484 0.61 +1.0 5,217,476 - Pf6N2E2_4484 0.61 +0.7 5,217,592 + Pf6N2E2_4484 0.68 +1.4 5,217,593 - Pf6N2E2_4484 0.68 +1.8 5,217,644 - Pf6N2E2_4484 0.71 +0.6 5,217,644 - Pf6N2E2_4484 0.71 -1.2 5,217,739 + Pf6N2E2_4484 0.77 -1.2 5,217,766 + Pf6N2E2_4484 0.79 +0.3 5,217,767 - Pf6N2E2_4484 0.79 +0.6 5,217,865 + Pf6N2E2_4484 0.85 +0.4 5,217,865 + Pf6N2E2_4484 0.85 +0.5 5,217,865 + Pf6N2E2_4484 0.85 +0.1 5,217,865 + Pf6N2E2_4484 0.85 +0.7 5,217,866 - Pf6N2E2_4484 0.85 -1.2 5,217,866 - Pf6N2E2_4484 0.85 +1.2 5,218,049 + +1.5 5,218,083 - +0.7 5,218,083 - -0.6 5,218,108 + -0.4 5,218,108 + -0.0 5,218,108 + -1.9 5,218,108 + +0.1 5,218,109 - -0.4 5,218,122 - +1.8 5,218,122 - +0.2 5,218,134 + +0.9 5,218,134 + -0.4 5,218,134 + +0.6 5,218,135 - +2.2 5,218,277 + -2.0 5,218,277 + +0.5 5,218,277 + -0.2 5,218,278 - +0.7 5,218,278 - +0.8 5,218,287 + -0.8 5,218,287 + +0.2 5,218,287 + -2.5 5,218,288 - +0.4 5,218,288 - +0.1 5,218,288 - +0.5 5,218,288 - +2.1 5,218,412 - Pf6N2E2_4485 0.13 +0.7 5,218,412 - Pf6N2E2_4485 0.13 +0.7 5,218,412 - Pf6N2E2_4485 0.13 +0.5 5,218,518 + Pf6N2E2_4485 0.23 +0.4 5,218,518 + Pf6N2E2_4485 0.23 -0.7 5,218,519 - Pf6N2E2_4485 0.23 +1.0 5,218,565 + Pf6N2E2_4485 0.28 +0.4 5,218,565 + Pf6N2E2_4485 0.28 +0.5 5,218,566 - Pf6N2E2_4485 0.28 -0.9 5,218,566 - Pf6N2E2_4485 0.28 +0.2 5,218,566 - Pf6N2E2_4485 0.28 +0.4 5,218,566 - Pf6N2E2_4485 0.28 +0.7 5,218,566 - Pf6N2E2_4485 0.28 +0.6 5,218,566 - Pf6N2E2_4485 0.28 +0.3 5,218,566 - Pf6N2E2_4485 0.28 -1.8 5,218,566 - Pf6N2E2_4485 0.28 -0.3 5,218,569 + Pf6N2E2_4485 0.28 +0.0 5,218,569 + Pf6N2E2_4485 0.28 -1.3 5,218,569 + Pf6N2E2_4485 0.28 +0.6 5,218,570 - Pf6N2E2_4485 0.28 -1.5 5,218,570 - Pf6N2E2_4485 0.28 +1.1 5,218,570 - Pf6N2E2_4485 0.28 -0.5 5,218,827 + Pf6N2E2_4485 0.52 -1.2 5,218,969 - Pf6N2E2_4485 0.65 -0.1 5,219,095 + Pf6N2E2_4485 0.77 -0.8 5,219,096 - Pf6N2E2_4485 0.77 +1.4 5,219,096 - Pf6N2E2_4485 0.77 -0.7 5,219,096 - Pf6N2E2_4485 0.77 -0.6 5,219,096 - Pf6N2E2_4485 0.77 -0.1 5,219,096 - Pf6N2E2_4485 0.77 +0.3 5,219,198 + Pf6N2E2_4485 0.86 -0.1 5,219,198 + Pf6N2E2_4485 0.86 +0.4 5,219,198 + Pf6N2E2_4485 0.86 -0.6 5,219,199 - Pf6N2E2_4485 0.86 +0.8 5,219,210 + Pf6N2E2_4485 0.87 -0.1 5,219,211 - Pf6N2E2_4485 0.87 +1.2 5,219,211 - Pf6N2E2_4485 0.87 -1.6 5,219,216 + Pf6N2E2_4485 0.88 -0.3 5,219,217 - Pf6N2E2_4485 0.88 +0.9 5,219,273 - -0.2 5,219,285 + +0.8 5,219,286 - +0.7 5,219,346 + -0.2 5,219,346 + -0.6 5,219,347 - -0.1 5,219,347 - +2.4 5,219,347 - +0.2 5,219,385 + +0.2 5,219,385 + +1.9 5,219,386 - +1.6 5,219,386 - +0.5 5,219,401 + +1.3 5,219,401 + -0.5 5,219,401 + -0.1 5,219,401 + +0.0 5,219,401 + +0.3 5,219,401 + +0.6 5,219,402 - +0.5 5,219,402 - -0.9 5,219,410 + -1.0 5,219,612 + Pf6N2E2_4486 0.55 +0.2 5,219,612 + Pf6N2E2_4486 0.55 -1.5 5,219,612 + Pf6N2E2_4486 0.55 -0.6 5,219,612 + Pf6N2E2_4486 0.55 -1.3 5,219,612 + Pf6N2E2_4486 0.55 -0.9 5,219,613 - Pf6N2E2_4486 0.55 -0.2 5,219,613 - Pf6N2E2_4486 0.55 -0.1 5,219,613 - Pf6N2E2_4486 0.55 -0.5 5,219,613 - Pf6N2E2_4486 0.55 +0.5 5,219,613 - Pf6N2E2_4486 0.55 -0.7 5,219,690 + Pf6N2E2_4486 0.75 -0.8 5,219,740 + Pf6N2E2_4486 0.88 +2.4 5,219,762 + -0.0 5,219,773 + -1.2 5,219,905 + +0.8 5,219,906 - +0.6 5,219,921 - -0.1 5,219,921 - -0.3 5,220,032 - +0.2 5,220,033 + +0.4 5,220,033 + +0.8 5,220,033 + -1.8 5,220,034 - +0.2 5,220,034 - -0.8 5,220,034 - -0.1 5,220,034 - -0.0 5,220,034 - -0.6 5,220,034 - -0.3 5,220,034 - -0.5 5,220,036 + +0.1 5,220,036 + +0.7 5,220,049 - +1.2 5,220,068 + +0.4 5,220,068 + +0.5 5,220,108 - -1.0 5,220,111 + +0.2 5,220,112 - +0.8 5,220,112 - +0.6 5,220,112 - -0.7 5,220,124 + +0.7 5,220,124 + -1.9 5,220,213 + +0.3 5,220,235 + +1.1 5,220,302 + Pf6N2E2_4487 0.15 +0.5 5,220,302 + Pf6N2E2_4487 0.15 -0.1 5,220,346 + Pf6N2E2_4487 0.19 +0.2 5,220,346 + Pf6N2E2_4487 0.19 +0.2
Or see this region's nucleotide sequence