Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4368 and Pf6N2E2_4369 are separated by 93 nucleotides Pf6N2E2_4369 and Pf6N2E2_4370 are separated by 83 nucleotides Pf6N2E2_4370 and Pf6N2E2_4371 are separated by 44 nucleotides
Pf6N2E2_4368 - Sodium/glutamate symport protein, at 5,096,800 to 5,098,008
_4368
Pf6N2E2_4369 - Exodeoxyribonuclease III (EC 3.1.11.2), at 5,098,102 to 5,098,881
_4369
Pf6N2E2_4370 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 5,098,965 to 5,099,609
_4370
Pf6N2E2_4371 - hypothetical protein, at 5,099,654 to 5,099,968
_4371
Position (kb)
5098
5099 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.601 kb on + strand, within Pf6N2E2_4368 at 5097.601 kb on + strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.629 kb on + strand, within Pf6N2E2_4368 at 5097.630 kb on - strand, within Pf6N2E2_4368 at 5097.630 kb on - strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.905 kb on + strand at 5097.905 kb on + strand at 5097.948 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5098.033 kb on + strand at 5098.046 kb on + strand at 5098.047 kb on - strand at 5098.047 kb on - strand at 5098.047 kb on - strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.448 kb on + strand, within Pf6N2E2_4369 at 5098.448 kb on + strand, within Pf6N2E2_4369 at 5098.449 kb on - strand, within Pf6N2E2_4369 at 5098.449 kb on - strand, within Pf6N2E2_4369 at 5098.715 kb on + strand, within Pf6N2E2_4369 at 5098.724 kb on + strand, within Pf6N2E2_4369 at 5098.724 kb on + strand, within Pf6N2E2_4369 at 5098.725 kb on - strand, within Pf6N2E2_4369 at 5098.725 kb on - strand, within Pf6N2E2_4369 at 5098.881 kb on + strand at 5098.882 kb on - strand at 5098.882 kb on - strand at 5098.908 kb on + strand at 5098.909 kb on - strand at 5098.929 kb on - strand at 5098.932 kb on + strand at 5098.936 kb on + strand at 5098.936 kb on + strand at 5098.947 kb on + strand at 5099.061 kb on + strand at 5099.178 kb on + strand, within Pf6N2E2_4370 at 5099.587 kb on - strand at 5099.641 kb on + strand at 5099.641 kb on + strand at 5099.681 kb on + strand at 5099.681 kb on + strand at 5099.681 kb on + strand at 5099.682 kb on - strand at 5099.769 kb on - strand, within Pf6N2E2_4371 at 5099.785 kb on - strand, within Pf6N2E2_4371
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,097,308 + Pf6N2E2_4368 0.42 +0.0 5,097,308 + Pf6N2E2_4368 0.42 -0.2 5,097,308 + Pf6N2E2_4368 0.42 -3.3 5,097,309 - Pf6N2E2_4368 0.42 +0.0 5,097,309 - Pf6N2E2_4368 0.42 -1.4 5,097,309 - Pf6N2E2_4368 0.42 +0.6 5,097,309 - Pf6N2E2_4368 0.42 +0.1 5,097,309 - Pf6N2E2_4368 0.42 +0.8 5,097,309 - Pf6N2E2_4368 0.42 -0.6 5,097,601 + Pf6N2E2_4368 0.66 +0.3 5,097,601 + Pf6N2E2_4368 0.66 +1.3 5,097,602 - Pf6N2E2_4368 0.66 +0.3 5,097,602 - Pf6N2E2_4368 0.66 -0.0 5,097,602 - Pf6N2E2_4368 0.66 +1.1 5,097,602 - Pf6N2E2_4368 0.66 -1.0 5,097,602 - Pf6N2E2_4368 0.66 -0.6 5,097,623 + Pf6N2E2_4368 0.68 +1.2 5,097,623 + Pf6N2E2_4368 0.68 -1.7 5,097,623 + Pf6N2E2_4368 0.68 +0.3 5,097,624 - Pf6N2E2_4368 0.68 -0.1 5,097,624 - Pf6N2E2_4368 0.68 +1.5 5,097,624 - Pf6N2E2_4368 0.68 -1.1 5,097,624 - Pf6N2E2_4368 0.68 +0.6 5,097,629 + Pf6N2E2_4368 0.69 -1.3 5,097,630 - Pf6N2E2_4368 0.69 +1.9 5,097,630 - Pf6N2E2_4368 0.69 +1.0 5,097,796 + Pf6N2E2_4368 0.82 +0.6 5,097,796 + Pf6N2E2_4368 0.82 -1.6 5,097,796 + Pf6N2E2_4368 0.82 +0.8 5,097,905 + +0.2 5,097,905 + -1.5 5,097,948 + +0.1 5,097,976 + -0.4 5,097,976 + -0.8 5,097,976 + +0.3 5,097,976 + -2.0 5,098,033 + -1.1 5,098,046 + -2.5 5,098,047 - -3.5 5,098,047 - -3.1 5,098,047 - -3.0 5,098,103 + -1.3 5,098,103 + -4.1 5,098,103 + -2.9 5,098,103 + -4.1 5,098,103 + -1.7 5,098,104 - -3.2 5,098,104 - -3.3 5,098,104 - -2.3 5,098,104 - -1.0 5,098,104 - -2.4 5,098,104 - -1.8 5,098,104 - +0.0 5,098,448 + Pf6N2E2_4369 0.44 +0.3 5,098,448 + Pf6N2E2_4369 0.44 -2.0 5,098,449 - Pf6N2E2_4369 0.44 -1.3 5,098,449 - Pf6N2E2_4369 0.44 -1.7 5,098,715 + Pf6N2E2_4369 0.79 -0.5 5,098,724 + Pf6N2E2_4369 0.80 +1.9 5,098,724 + Pf6N2E2_4369 0.80 -1.7 5,098,725 - Pf6N2E2_4369 0.80 -0.5 5,098,725 - Pf6N2E2_4369 0.80 -1.3 5,098,881 + -3.3 5,098,882 - -1.1 5,098,882 - -0.1 5,098,908 + -1.0 5,098,909 - -0.5 5,098,929 - +0.3 5,098,932 + -1.2 5,098,936 + -2.3 5,098,936 + -2.9 5,098,947 + -1.1 5,099,061 + +0.3 5,099,178 + Pf6N2E2_4370 0.33 -0.7 5,099,587 - -2.2 5,099,641 + -0.3 5,099,641 + -0.6 5,099,681 + -1.8 5,099,681 + -3.6 5,099,681 + -0.5 5,099,682 - +0.3 5,099,769 - Pf6N2E2_4371 0.37 +0.9 5,099,785 - Pf6N2E2_4371 0.42 -0.4
Or see this region's nucleotide sequence