Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4365 and Pf6N2E2_4366 are separated by 31 nucleotides Pf6N2E2_4366 and Pf6N2E2_4367 are separated by 140 nucleotides Pf6N2E2_4367 and Pf6N2E2_4368 are separated by 66 nucleotides Pf6N2E2_4368 and Pf6N2E2_4369 are separated by 93 nucleotides Pf6N2E2_4369 and Pf6N2E2_4370 are separated by 83 nucleotides
Pf6N2E2_4365 - Ribonuclease PH (EC 2.7.7.56), at 5,095,315 to 5,096,037
_4365
Pf6N2E2_4366 - FIG00955116: hypothetical protein, at 5,096,069 to 5,096,437
_4366
Pf6N2E2_4367 - hypothetical protein, at 5,096,578 to 5,096,733
_4367
Pf6N2E2_4368 - Sodium/glutamate symport protein, at 5,096,800 to 5,098,008
_4368
Pf6N2E2_4369 - Exodeoxyribonuclease III (EC 3.1.11.2), at 5,098,102 to 5,098,881
_4369
Pf6N2E2_4370 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 5,098,965 to 5,099,609
_4370
Position (kb)
5096
5097
5098
5099 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.969 kb on - strand at 5096.416 kb on + strand at 5096.417 kb on - strand at 5096.546 kb on - strand at 5096.546 kb on - strand at 5096.576 kb on - strand at 5096.607 kb on + strand, within Pf6N2E2_4367 at 5096.608 kb on - strand, within Pf6N2E2_4367 at 5096.653 kb on - strand, within Pf6N2E2_4367 at 5096.758 kb on - strand at 5096.758 kb on - strand at 5096.777 kb on - strand at 5096.778 kb on + strand at 5096.778 kb on + strand at 5096.779 kb on - strand at 5096.818 kb on + strand at 5096.871 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.902 kb on - strand at 5096.916 kb on - strand at 5096.930 kb on + strand, within Pf6N2E2_4368 at 5096.930 kb on + strand, within Pf6N2E2_4368 at 5096.931 kb on - strand, within Pf6N2E2_4368 at 5096.931 kb on - strand, within Pf6N2E2_4368 at 5096.985 kb on + strand, within Pf6N2E2_4368 at 5096.986 kb on - strand, within Pf6N2E2_4368 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.308 kb on + strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.309 kb on - strand, within Pf6N2E2_4368 at 5097.601 kb on + strand, within Pf6N2E2_4368 at 5097.601 kb on + strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.602 kb on - strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.623 kb on + strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.624 kb on - strand, within Pf6N2E2_4368 at 5097.629 kb on + strand, within Pf6N2E2_4368 at 5097.630 kb on - strand, within Pf6N2E2_4368 at 5097.630 kb on - strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.796 kb on + strand, within Pf6N2E2_4368 at 5097.905 kb on + strand at 5097.905 kb on + strand at 5097.948 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5097.976 kb on + strand at 5098.033 kb on + strand at 5098.046 kb on + strand at 5098.047 kb on - strand at 5098.047 kb on - strand at 5098.047 kb on - strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.103 kb on + strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.104 kb on - strand at 5098.448 kb on + strand, within Pf6N2E2_4369 at 5098.448 kb on + strand, within Pf6N2E2_4369 at 5098.449 kb on - strand, within Pf6N2E2_4369 at 5098.449 kb on - strand, within Pf6N2E2_4369 at 5098.715 kb on + strand, within Pf6N2E2_4369 at 5098.724 kb on + strand, within Pf6N2E2_4369 at 5098.724 kb on + strand, within Pf6N2E2_4369 at 5098.725 kb on - strand, within Pf6N2E2_4369 at 5098.725 kb on - strand, within Pf6N2E2_4369 at 5098.881 kb on + strand at 5098.882 kb on - strand at 5098.882 kb on - strand at 5098.908 kb on + strand at 5098.909 kb on - strand at 5098.929 kb on - strand at 5098.932 kb on + strand at 5098.936 kb on + strand at 5098.936 kb on + strand at 5098.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,095,819 + Pf6N2E2_4365 0.70 +4.2 5,095,819 + Pf6N2E2_4365 0.70 +4.3 5,095,819 + Pf6N2E2_4365 0.70 +4.3 5,095,820 - Pf6N2E2_4365 0.70 +3.4 5,095,820 - Pf6N2E2_4365 0.70 +4.6 5,095,820 - Pf6N2E2_4365 0.70 +4.0 5,095,820 - Pf6N2E2_4365 0.70 +4.6 5,095,820 - Pf6N2E2_4365 0.70 +3.8 5,095,820 - Pf6N2E2_4365 0.70 +3.5 5,095,849 + Pf6N2E2_4365 0.74 +4.7 5,095,849 + Pf6N2E2_4365 0.74 +4.4 5,095,849 + Pf6N2E2_4365 0.74 +3.7 5,095,850 - Pf6N2E2_4365 0.74 +4.9 5,095,850 - Pf6N2E2_4365 0.74 +4.6 5,095,850 - Pf6N2E2_4365 0.74 +2.7 5,095,886 + Pf6N2E2_4365 0.79 +4.0 5,095,886 + Pf6N2E2_4365 0.79 +5.1 5,095,887 - Pf6N2E2_4365 0.79 +4.2 5,095,887 - Pf6N2E2_4365 0.79 +3.5 5,095,969 - +4.1 5,096,416 + +0.6 5,096,417 - +0.7 5,096,546 - +0.7 5,096,546 - +1.4 5,096,576 - +0.6 5,096,607 + Pf6N2E2_4367 0.19 +1.2 5,096,608 - Pf6N2E2_4367 0.19 +0.9 5,096,653 - Pf6N2E2_4367 0.48 +0.3 5,096,758 - +0.9 5,096,758 - +0.0 5,096,777 - +1.1 5,096,778 + +0.6 5,096,778 + +1.9 5,096,779 - +1.0 5,096,818 + +0.0 5,096,871 + -0.2 5,096,896 + +0.1 5,096,896 + +0.3 5,096,896 + +0.3 5,096,896 + -0.6 5,096,902 - +1.0 5,096,916 - -1.1 5,096,930 + Pf6N2E2_4368 0.11 +1.4 5,096,930 + Pf6N2E2_4368 0.11 -0.2 5,096,931 - Pf6N2E2_4368 0.11 +1.1 5,096,931 - Pf6N2E2_4368 0.11 +0.2 5,096,985 + Pf6N2E2_4368 0.15 +0.6 5,096,986 - Pf6N2E2_4368 0.15 +0.1 5,097,308 + Pf6N2E2_4368 0.42 +0.0 5,097,308 + Pf6N2E2_4368 0.42 -0.2 5,097,308 + Pf6N2E2_4368 0.42 -3.3 5,097,309 - Pf6N2E2_4368 0.42 +0.0 5,097,309 - Pf6N2E2_4368 0.42 -1.4 5,097,309 - Pf6N2E2_4368 0.42 +0.6 5,097,309 - Pf6N2E2_4368 0.42 +0.1 5,097,309 - Pf6N2E2_4368 0.42 +0.8 5,097,309 - Pf6N2E2_4368 0.42 -0.6 5,097,601 + Pf6N2E2_4368 0.66 +0.3 5,097,601 + Pf6N2E2_4368 0.66 +1.3 5,097,602 - Pf6N2E2_4368 0.66 +0.3 5,097,602 - Pf6N2E2_4368 0.66 -0.0 5,097,602 - Pf6N2E2_4368 0.66 +1.1 5,097,602 - Pf6N2E2_4368 0.66 -1.0 5,097,602 - Pf6N2E2_4368 0.66 -0.6 5,097,623 + Pf6N2E2_4368 0.68 +1.2 5,097,623 + Pf6N2E2_4368 0.68 -1.7 5,097,623 + Pf6N2E2_4368 0.68 +0.3 5,097,624 - Pf6N2E2_4368 0.68 -0.1 5,097,624 - Pf6N2E2_4368 0.68 +1.5 5,097,624 - Pf6N2E2_4368 0.68 -1.1 5,097,624 - Pf6N2E2_4368 0.68 +0.6 5,097,629 + Pf6N2E2_4368 0.69 -1.3 5,097,630 - Pf6N2E2_4368 0.69 +1.9 5,097,630 - Pf6N2E2_4368 0.69 +1.0 5,097,796 + Pf6N2E2_4368 0.82 +0.6 5,097,796 + Pf6N2E2_4368 0.82 -1.6 5,097,796 + Pf6N2E2_4368 0.82 +0.8 5,097,905 + +0.2 5,097,905 + -1.5 5,097,948 + +0.1 5,097,976 + -0.4 5,097,976 + -0.8 5,097,976 + +0.3 5,097,976 + -2.0 5,098,033 + -1.1 5,098,046 + -2.5 5,098,047 - -3.5 5,098,047 - -3.1 5,098,047 - -3.0 5,098,103 + -1.3 5,098,103 + -4.1 5,098,103 + -2.9 5,098,103 + -4.1 5,098,103 + -1.7 5,098,104 - -3.2 5,098,104 - -3.3 5,098,104 - -2.3 5,098,104 - -1.0 5,098,104 - -2.4 5,098,104 - -1.8 5,098,104 - +0.0 5,098,448 + Pf6N2E2_4369 0.44 +0.3 5,098,448 + Pf6N2E2_4369 0.44 -2.0 5,098,449 - Pf6N2E2_4369 0.44 -1.3 5,098,449 - Pf6N2E2_4369 0.44 -1.7 5,098,715 + Pf6N2E2_4369 0.79 -0.5 5,098,724 + Pf6N2E2_4369 0.80 +1.9 5,098,724 + Pf6N2E2_4369 0.80 -1.7 5,098,725 - Pf6N2E2_4369 0.80 -0.5 5,098,725 - Pf6N2E2_4369 0.80 -1.3 5,098,881 + -3.3 5,098,882 - -1.1 5,098,882 - -0.1 5,098,908 + -1.0 5,098,909 - -0.5 5,098,929 - +0.3 5,098,932 + -1.2 5,098,936 + -2.3 5,098,936 + -2.9 5,098,947 + -1.1
Or see this region's nucleotide sequence