Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4364 and Pf6N2E2_4365 are separated by 271 nucleotides Pf6N2E2_4365 and Pf6N2E2_4366 are separated by 31 nucleotides Pf6N2E2_4366 and Pf6N2E2_4367 are separated by 140 nucleotides Pf6N2E2_4367 and Pf6N2E2_4368 are separated by 66 nucleotides
Pf6N2E2_4364 - Protein YicC, at 5,094,180 to 5,095,043
_4364
Pf6N2E2_4365 - Ribonuclease PH (EC 2.7.7.56), at 5,095,315 to 5,096,037
_4365
Pf6N2E2_4366 - FIG00955116: hypothetical protein, at 5,096,069 to 5,096,437
_4366
Pf6N2E2_4367 - hypothetical protein, at 5,096,578 to 5,096,733
_4367
Pf6N2E2_4368 - Sodium/glutamate symport protein, at 5,096,800 to 5,098,008
_4368
Position (kb)
5095
5096
5097 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 5095.134 kb on + strand at 5095.135 kb on - strand at 5095.254 kb on + strand at 5095.272 kb on + strand at 5095.272 kb on + strand at 5095.272 kb on + strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.307 kb on + strand at 5095.307 kb on + strand at 5095.366 kb on - strand at 5095.375 kb on + strand at 5095.376 kb on - strand at 5095.376 kb on - strand at 5095.401 kb on - strand, within Pf6N2E2_4365 at 5095.401 kb on - strand, within Pf6N2E2_4365 at 5095.420 kb on - strand, within Pf6N2E2_4365 at 5095.420 kb on - strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.969 kb on - strand at 5096.416 kb on + strand at 5096.417 kb on - strand at 5096.546 kb on - strand at 5096.546 kb on - strand at 5096.576 kb on - strand at 5096.607 kb on + strand, within Pf6N2E2_4367 at 5096.608 kb on - strand, within Pf6N2E2_4367 at 5096.653 kb on - strand, within Pf6N2E2_4367 at 5096.758 kb on - strand at 5096.758 kb on - strand at 5096.777 kb on - strand at 5096.778 kb on + strand at 5096.778 kb on + strand at 5096.779 kb on - strand at 5096.818 kb on + strand at 5096.871 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.896 kb on + strand at 5096.902 kb on - strand at 5096.916 kb on - strand at 5096.930 kb on + strand, within Pf6N2E2_4368 at 5096.930 kb on + strand, within Pf6N2E2_4368 at 5096.931 kb on - strand, within Pf6N2E2_4368 at 5096.931 kb on - strand, within Pf6N2E2_4368 at 5096.985 kb on + strand, within Pf6N2E2_4368 at 5096.986 kb on - strand, within Pf6N2E2_4368
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 5,095,134 + -2.2 5,095,135 - +0.2 5,095,254 + +1.5 5,095,272 + +1.0 5,095,272 + -0.4 5,095,272 + +0.4 5,095,273 - +1.9 5,095,273 - +1.3 5,095,273 - +2.0 5,095,273 - +1.6 5,095,274 + +1.0 5,095,274 + +2.3 5,095,274 + +0.8 5,095,274 + +0.4 5,095,307 + +4.3 5,095,307 + +4.5 5,095,366 - +3.7 5,095,375 + +3.4 5,095,376 - +4.2 5,095,376 - +4.1 5,095,401 - Pf6N2E2_4365 0.12 +6.3 5,095,401 - Pf6N2E2_4365 0.12 +2.4 5,095,420 - Pf6N2E2_4365 0.15 +3.8 5,095,420 - Pf6N2E2_4365 0.15 +2.7 5,095,654 + Pf6N2E2_4365 0.47 +4.3 5,095,654 + Pf6N2E2_4365 0.47 +2.4 5,095,654 + Pf6N2E2_4365 0.47 +5.0 5,095,655 - Pf6N2E2_4365 0.47 +3.7 5,095,655 - Pf6N2E2_4365 0.47 +4.0 5,095,655 - Pf6N2E2_4365 0.47 +3.9 5,095,819 + Pf6N2E2_4365 0.70 +4.2 5,095,819 + Pf6N2E2_4365 0.70 +4.3 5,095,819 + Pf6N2E2_4365 0.70 +4.3 5,095,820 - Pf6N2E2_4365 0.70 +3.4 5,095,820 - Pf6N2E2_4365 0.70 +4.6 5,095,820 - Pf6N2E2_4365 0.70 +4.0 5,095,820 - Pf6N2E2_4365 0.70 +4.6 5,095,820 - Pf6N2E2_4365 0.70 +3.8 5,095,820 - Pf6N2E2_4365 0.70 +3.5 5,095,849 + Pf6N2E2_4365 0.74 +4.7 5,095,849 + Pf6N2E2_4365 0.74 +4.4 5,095,849 + Pf6N2E2_4365 0.74 +3.7 5,095,850 - Pf6N2E2_4365 0.74 +4.9 5,095,850 - Pf6N2E2_4365 0.74 +4.6 5,095,850 - Pf6N2E2_4365 0.74 +2.7 5,095,886 + Pf6N2E2_4365 0.79 +4.0 5,095,886 + Pf6N2E2_4365 0.79 +5.1 5,095,887 - Pf6N2E2_4365 0.79 +4.2 5,095,887 - Pf6N2E2_4365 0.79 +3.5 5,095,969 - +4.1 5,096,416 + +0.6 5,096,417 - +0.7 5,096,546 - +0.7 5,096,546 - +1.4 5,096,576 - +0.6 5,096,607 + Pf6N2E2_4367 0.19 +1.2 5,096,608 - Pf6N2E2_4367 0.19 +0.9 5,096,653 - Pf6N2E2_4367 0.48 +0.3 5,096,758 - +0.9 5,096,758 - +0.0 5,096,777 - +1.1 5,096,778 + +0.6 5,096,778 + +1.9 5,096,779 - +1.0 5,096,818 + +0.0 5,096,871 + -0.2 5,096,896 + +0.1 5,096,896 + +0.3 5,096,896 + +0.3 5,096,896 + -0.6 5,096,902 - +1.0 5,096,916 - -1.1 5,096,930 + Pf6N2E2_4368 0.11 +1.4 5,096,930 + Pf6N2E2_4368 0.11 -0.2 5,096,931 - Pf6N2E2_4368 0.11 +1.1 5,096,931 - Pf6N2E2_4368 0.11 +0.2 5,096,985 + Pf6N2E2_4368 0.15 +0.6 5,096,986 - Pf6N2E2_4368 0.15 +0.1
Or see this region's nucleotide sequence