Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4165 and Pf6N2E2_4166 are separated by 114 nucleotides Pf6N2E2_4166 and Pf6N2E2_4167 overlap by 1 nucleotides Pf6N2E2_4167 and Pf6N2E2_4168 overlap by 8 nucleotides Pf6N2E2_4168 and Pf6N2E2_4169 are separated by 37 nucleotides
Pf6N2E2_4165 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 4,847,624 to 4,848,553
_4165
Pf6N2E2_4166 - Zinc uptake regulation protein ZUR, at 4,848,668 to 4,849,102
_4166
Pf6N2E2_4167 - Zinc ABC transporter, ATP-binding protein ZnuC, at 4,849,102 to 4,849,884
_4167
Pf6N2E2_4168 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 4,849,877 to 4,850,665
_4168
Pf6N2E2_4169 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,850,703 to 4,851,416
_4169
Position (kb)
4849
4850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4848.322 kb on - strand, within Pf6N2E2_4165 at 4848.372 kb on + strand, within Pf6N2E2_4165 at 4848.373 kb on - strand, within Pf6N2E2_4165 at 4848.507 kb on - strand at 4848.566 kb on + strand at 4848.566 kb on + strand at 4848.566 kb on + strand at 4848.567 kb on - strand at 4848.587 kb on + strand at 4848.588 kb on - strand at 4848.625 kb on + strand at 4848.625 kb on + strand at 4848.626 kb on - strand at 4848.626 kb on - strand at 4848.626 kb on - strand at 4848.628 kb on + strand at 4848.628 kb on + strand at 4848.628 kb on + strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.316 kb on - strand, within Pf6N2E2_4167 at 4849.317 kb on + strand, within Pf6N2E2_4167 at 4849.317 kb on + strand, within Pf6N2E2_4167 at 4849.318 kb on - strand, within Pf6N2E2_4167 at 4849.318 kb on - strand, within Pf6N2E2_4167 at 4849.490 kb on + strand, within Pf6N2E2_4167 at 4849.490 kb on + strand, within Pf6N2E2_4167 at 4849.582 kb on + strand, within Pf6N2E2_4167 at 4849.583 kb on - strand, within Pf6N2E2_4167 at 4849.697 kb on + strand, within Pf6N2E2_4167 at 4849.697 kb on + strand, within Pf6N2E2_4167 at 4849.697 kb on + strand, within Pf6N2E2_4167 at 4849.697 kb on + strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.698 kb on - strand, within Pf6N2E2_4167 at 4849.783 kb on + strand, within Pf6N2E2_4167 at 4849.783 kb on + strand, within Pf6N2E2_4167 at 4849.783 kb on + strand, within Pf6N2E2_4167 at 4849.783 kb on + strand, within Pf6N2E2_4167 at 4849.784 kb on - strand, within Pf6N2E2_4167 at 4849.784 kb on - strand, within Pf6N2E2_4167 at 4849.896 kb on - strand at 4849.896 kb on - strand at 4849.973 kb on + strand, within Pf6N2E2_4168 at 4849.973 kb on + strand, within Pf6N2E2_4168 at 4850.085 kb on + strand, within Pf6N2E2_4168 at 4850.085 kb on + strand, within Pf6N2E2_4168 at 4850.086 kb on - strand, within Pf6N2E2_4168 at 4850.175 kb on + strand, within Pf6N2E2_4168 at 4850.176 kb on - strand, within Pf6N2E2_4168 at 4850.178 kb on + strand, within Pf6N2E2_4168 at 4850.178 kb on + strand, within Pf6N2E2_4168 at 4850.178 kb on + strand, within Pf6N2E2_4168 at 4850.178 kb on + strand, within Pf6N2E2_4168 at 4850.179 kb on - strand, within Pf6N2E2_4168 at 4850.179 kb on - strand, within Pf6N2E2_4168 at 4850.179 kb on - strand, within Pf6N2E2_4168 at 4850.179 kb on - strand, within Pf6N2E2_4168 at 4850.216 kb on + strand, within Pf6N2E2_4168 at 4850.216 kb on + strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.217 kb on - strand, within Pf6N2E2_4168 at 4850.452 kb on + strand, within Pf6N2E2_4168 at 4850.453 kb on - strand, within Pf6N2E2_4168 at 4850.647 kb on - strand at 4850.663 kb on + strand at 4850.663 kb on + strand at 4850.663 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.771 kb on + strand at 4850.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,848,322 - Pf6N2E2_4165 0.75 -1.8 4,848,372 + Pf6N2E2_4165 0.80 -0.6 4,848,373 - Pf6N2E2_4165 0.81 -0.5 4,848,507 - -2.5 4,848,566 + -1.8 4,848,566 + -1.1 4,848,566 + -0.8 4,848,567 - +0.9 4,848,587 + -2.0 4,848,588 - -2.1 4,848,625 + -3.3 4,848,625 + -1.5 4,848,626 - +0.0 4,848,626 - -1.6 4,848,626 - -1.5 4,848,628 + -0.2 4,848,628 + -2.4 4,848,628 + -1.2 4,848,629 - -2.2 4,848,629 - -0.7 4,848,629 - -1.3 4,848,629 - -2.2 4,848,629 - -1.6 4,849,315 + Pf6N2E2_4167 0.27 -2.5 4,849,315 + Pf6N2E2_4167 0.27 -0.6 4,849,315 + Pf6N2E2_4167 0.27 -1.5 4,849,316 - Pf6N2E2_4167 0.27 -1.5 4,849,317 + Pf6N2E2_4167 0.27 -0.9 4,849,317 + Pf6N2E2_4167 0.27 -0.4 4,849,318 - Pf6N2E2_4167 0.28 -2.0 4,849,318 - Pf6N2E2_4167 0.28 -0.3 4,849,490 + Pf6N2E2_4167 0.50 -0.6 4,849,490 + Pf6N2E2_4167 0.50 -0.9 4,849,582 + Pf6N2E2_4167 0.61 -0.9 4,849,583 - Pf6N2E2_4167 0.61 -0.6 4,849,697 + Pf6N2E2_4167 0.76 -2.7 4,849,697 + Pf6N2E2_4167 0.76 -1.2 4,849,697 + Pf6N2E2_4167 0.76 -1.4 4,849,697 + Pf6N2E2_4167 0.76 -0.6 4,849,698 - Pf6N2E2_4167 0.76 -0.9 4,849,698 - Pf6N2E2_4167 0.76 -1.9 4,849,698 - Pf6N2E2_4167 0.76 -0.1 4,849,698 - Pf6N2E2_4167 0.76 +0.2 4,849,698 - Pf6N2E2_4167 0.76 -2.5 4,849,698 - Pf6N2E2_4167 0.76 -0.9 4,849,698 - Pf6N2E2_4167 0.76 -0.7 4,849,698 - Pf6N2E2_4167 0.76 +0.1 4,849,698 - Pf6N2E2_4167 0.76 +0.1 4,849,698 - Pf6N2E2_4167 0.76 -0.4 4,849,698 - Pf6N2E2_4167 0.76 +0.2 4,849,783 + Pf6N2E2_4167 0.87 +0.4 4,849,783 + Pf6N2E2_4167 0.87 -1.3 4,849,783 + Pf6N2E2_4167 0.87 -0.3 4,849,783 + Pf6N2E2_4167 0.87 +0.6 4,849,784 - Pf6N2E2_4167 0.87 -0.7 4,849,784 - Pf6N2E2_4167 0.87 -0.8 4,849,896 - +0.0 4,849,896 - -1.5 4,849,973 + Pf6N2E2_4168 0.12 -1.2 4,849,973 + Pf6N2E2_4168 0.12 -0.3 4,850,085 + Pf6N2E2_4168 0.26 -0.4 4,850,085 + Pf6N2E2_4168 0.26 +0.4 4,850,086 - Pf6N2E2_4168 0.26 -0.6 4,850,175 + Pf6N2E2_4168 0.38 +0.6 4,850,176 - Pf6N2E2_4168 0.38 +1.9 4,850,178 + Pf6N2E2_4168 0.38 -1.9 4,850,178 + Pf6N2E2_4168 0.38 -2.2 4,850,178 + Pf6N2E2_4168 0.38 -2.1 4,850,178 + Pf6N2E2_4168 0.38 -0.5 4,850,179 - Pf6N2E2_4168 0.38 -0.9 4,850,179 - Pf6N2E2_4168 0.38 -1.6 4,850,179 - Pf6N2E2_4168 0.38 +0.1 4,850,179 - Pf6N2E2_4168 0.38 -1.2 4,850,216 + Pf6N2E2_4168 0.43 -1.0 4,850,216 + Pf6N2E2_4168 0.43 +0.0 4,850,217 - Pf6N2E2_4168 0.43 -0.3 4,850,217 - Pf6N2E2_4168 0.43 -2.3 4,850,217 - Pf6N2E2_4168 0.43 -0.2 4,850,217 - Pf6N2E2_4168 0.43 +0.8 4,850,217 - Pf6N2E2_4168 0.43 -2.1 4,850,217 - Pf6N2E2_4168 0.43 +0.8 4,850,452 + Pf6N2E2_4168 0.73 -0.5 4,850,453 - Pf6N2E2_4168 0.73 +0.9 4,850,647 - -1.2 4,850,663 + +0.2 4,850,663 + -1.6 4,850,663 + +0.3 4,850,688 + -0.8 4,850,688 + +0.6 4,850,688 + +0.5 4,850,688 + +1.9 4,850,771 + -1.5 4,850,772 - -3.3
Or see this region's nucleotide sequence