Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4161 and Pf6N2E2_4162 are separated by 374 nucleotides Pf6N2E2_4162 and Pf6N2E2_4163 are separated by 74 nucleotides Pf6N2E2_4163 and Pf6N2E2_4164 are separated by 59 nucleotides
Pf6N2E2_4161 - GTP-binding protein EngB, at 4,842,302 to 4,842,943
_4161
Pf6N2E2_4162 - DNA polymerase I (EC 2.7.7.7), at 4,843,318 to 4,846,086
_4162
Pf6N2E2_4163 - FIG00953549: hypothetical protein, at 4,846,161 to 4,846,448
_4163
Pf6N2E2_4164 - Homoserine kinase (EC 2.7.1.39), at 4,846,508 to 4,847,458
_4164
Position (kb)
4843
4844
4845
4846
4847 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4842.942 kb on - strand at 4842.979 kb on - strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.117 kb on + strand at 4843.118 kb on - strand at 4843.127 kb on + strand at 4843.139 kb on + strand at 4843.140 kb on - strand at 4843.140 kb on - strand at 4843.161 kb on + strand at 4843.161 kb on + strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.313 kb on - strand at 4844.187 kb on - strand, within Pf6N2E2_4162 at 4844.187 kb on - strand, within Pf6N2E2_4162 at 4844.768 kb on + strand, within Pf6N2E2_4162 at 4844.768 kb on + strand, within Pf6N2E2_4162 at 4845.144 kb on - strand, within Pf6N2E2_4162 at 4845.144 kb on - strand, within Pf6N2E2_4162 at 4846.121 kb on - strand at 4846.157 kb on + strand at 4846.157 kb on + strand at 4846.158 kb on - strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.167 kb on - strand at 4846.167 kb on - strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.172 kb on - strand at 4846.280 kb on + strand, within Pf6N2E2_4163 at 4846.318 kb on - strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.435 kb on + strand at 4846.435 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,842,942 - -0.4 4,842,979 - -1.2 4,843,034 + -0.2 4,843,034 + -0.3 4,843,034 + +1.0 4,843,034 + -1.7 4,843,048 + -0.3 4,843,048 + -0.6 4,843,048 + +0.1 4,843,048 + -3.6 4,843,048 + -0.1 4,843,048 + -0.6 4,843,048 + -0.1 4,843,049 - -3.5 4,843,049 - -0.4 4,843,049 - +1.6 4,843,049 - -1.3 4,843,049 - -1.3 4,843,049 - -2.2 4,843,049 - -0.1 4,843,049 - -0.9 4,843,058 + +0.1 4,843,058 + +0.2 4,843,058 + +0.5 4,843,058 + -1.3 4,843,058 + +0.8 4,843,058 + -1.3 4,843,058 + -0.4 4,843,058 + -0.6 4,843,059 - -0.2 4,843,059 - -0.3 4,843,059 - -1.4 4,843,059 - -0.1 4,843,059 - +0.1 4,843,059 - -2.8 4,843,059 - -1.2 4,843,117 + -0.2 4,843,118 - +1.5 4,843,127 + -1.5 4,843,139 + -3.3 4,843,140 - -1.5 4,843,140 - +0.1 4,843,161 + -2.1 4,843,161 + -0.6 4,843,162 - -1.2 4,843,162 - +1.5 4,843,162 - -0.2 4,843,162 - -0.0 4,843,192 + -0.5 4,843,192 + -0.2 4,843,192 + -0.5 4,843,192 + -0.7 4,843,192 + +0.2 4,843,192 + +0.1 4,843,192 + -1.5 4,843,192 + -1.4 4,843,193 - -0.3 4,843,193 - -0.4 4,843,193 - -1.6 4,843,193 - +0.4 4,843,193 - -1.0 4,843,193 - +0.3 4,843,193 - -1.1 4,843,193 - -1.4 4,843,193 - -0.8 4,843,193 - -0.2 4,843,193 - -0.6 4,843,193 - -1.5 4,843,193 - -0.2 4,843,193 - -0.9 4,843,193 - -0.8 4,843,313 - +0.5 4,844,187 - Pf6N2E2_4162 0.31 -1.4 4,844,187 - Pf6N2E2_4162 0.31 +0.4 4,844,768 + Pf6N2E2_4162 0.52 -0.9 4,844,768 + Pf6N2E2_4162 0.52 -1.1 4,845,144 - Pf6N2E2_4162 0.66 -3.5 4,845,144 - Pf6N2E2_4162 0.66 -0.2 4,846,121 - -0.2 4,846,157 + -1.0 4,846,157 + -1.2 4,846,158 - -1.8 4,846,166 + +0.6 4,846,166 + -0.1 4,846,166 + -1.1 4,846,166 + +0.2 4,846,167 - -1.5 4,846,167 - -0.0 4,846,171 + +0.7 4,846,171 + -0.3 4,846,171 + -1.1 4,846,172 - +0.5 4,846,280 + Pf6N2E2_4163 0.41 -1.0 4,846,318 - Pf6N2E2_4163 0.55 +2.4 4,846,353 + Pf6N2E2_4163 0.67 +2.6 4,846,353 + Pf6N2E2_4163 0.67 +0.6 4,846,353 + Pf6N2E2_4163 0.67 +0.3 4,846,353 + Pf6N2E2_4163 0.67 +0.5 4,846,353 + Pf6N2E2_4163 0.67 +0.3 4,846,435 + -1.4 4,846,435 + +0.0 4,846,446 + +1.1 4,846,446 + +0.4 4,846,446 + -0.2
Or see this region's nucleotide sequence