Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3906 and Pf6N2E2_3907 overlap by 1 nucleotides Pf6N2E2_3907 and Pf6N2E2_3908 are separated by 255 nucleotides Pf6N2E2_3908 and Pf6N2E2_3909 are separated by 250 nucleotides
Pf6N2E2_3906 - Transcription accessory protein (S1 RNA-binding domain), at 4,539,819 to 4,542,143
_3906
Pf6N2E2_3907 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 4,542,143 to 4,542,526
_3907
Pf6N2E2_3908 - Glutamate--cysteine ligase (EC 6.3.2.2), at 4,542,782 to 4,544,380
_3908
Pf6N2E2_3909 - Taurine-binding periplasmic protein TauA, at 4,544,631 to 4,545,608
_3909
Position (kb)
4542
4543
4544
4545 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4541.845 kb on + strand, within Pf6N2E2_3906 at 4541.845 kb on + strand, within Pf6N2E2_3906 at 4541.846 kb on - strand, within Pf6N2E2_3906 at 4541.846 kb on - strand, within Pf6N2E2_3906 at 4542.383 kb on + strand, within Pf6N2E2_3907 at 4542.383 kb on + strand, within Pf6N2E2_3907 at 4542.383 kb on + strand, within Pf6N2E2_3907 at 4542.383 kb on + strand, within Pf6N2E2_3907 at 4542.383 kb on + strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.384 kb on - strand, within Pf6N2E2_3907 at 4542.522 kb on + strand at 4542.522 kb on + strand at 4542.522 kb on + strand at 4542.523 kb on - strand at 4542.523 kb on - strand at 4542.524 kb on + strand at 4542.524 kb on + strand at 4542.525 kb on - strand at 4542.549 kb on - strand at 4542.764 kb on + strand at 4542.765 kb on - strand at 4542.984 kb on - strand, within Pf6N2E2_3908 at 4543.095 kb on - strand, within Pf6N2E2_3908 at 4543.095 kb on - strand, within Pf6N2E2_3908 at 4543.098 kb on + strand, within Pf6N2E2_3908 at 4543.098 kb on + strand, within Pf6N2E2_3908 at 4543.098 kb on + strand, within Pf6N2E2_3908 at 4543.098 kb on + strand, within Pf6N2E2_3908 at 4543.099 kb on - strand, within Pf6N2E2_3908 at 4543.157 kb on + strand, within Pf6N2E2_3908 at 4543.187 kb on + strand, within Pf6N2E2_3908 at 4543.187 kb on + strand, within Pf6N2E2_3908 at 4543.187 kb on + strand, within Pf6N2E2_3908 at 4543.188 kb on - strand, within Pf6N2E2_3908 at 4543.188 kb on - strand, within Pf6N2E2_3908 at 4543.188 kb on - strand, within Pf6N2E2_3908 at 4543.188 kb on - strand, within Pf6N2E2_3908 at 4543.344 kb on - strand, within Pf6N2E2_3908 at 4543.391 kb on + strand, within Pf6N2E2_3908 at 4543.392 kb on - strand, within Pf6N2E2_3908 at 4543.392 kb on - strand, within Pf6N2E2_3908 at 4543.478 kb on + strand, within Pf6N2E2_3908 at 4543.478 kb on + strand, within Pf6N2E2_3908 at 4543.557 kb on - strand, within Pf6N2E2_3908 at 4543.557 kb on - strand, within Pf6N2E2_3908 at 4543.666 kb on + strand, within Pf6N2E2_3908 at 4543.694 kb on + strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.727 kb on + strand, within Pf6N2E2_3908 at 4543.776 kb on - strand, within Pf6N2E2_3908 at 4543.865 kb on + strand, within Pf6N2E2_3908 at 4543.866 kb on - strand, within Pf6N2E2_3908 at 4544.349 kb on - strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.508 kb on - strand at 4544.770 kb on - strand, within Pf6N2E2_3909 at 4545.045 kb on + strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.100 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,541,845 + Pf6N2E2_3906 0.87 +0.6 4,541,845 + Pf6N2E2_3906 0.87 +0.7 4,541,846 - Pf6N2E2_3906 0.87 -1.6 4,541,846 - Pf6N2E2_3906 0.87 -2.4 4,542,383 + Pf6N2E2_3907 0.62 +0.6 4,542,383 + Pf6N2E2_3907 0.62 -1.0 4,542,383 + Pf6N2E2_3907 0.62 -0.2 4,542,383 + Pf6N2E2_3907 0.62 +1.6 4,542,383 + Pf6N2E2_3907 0.62 +0.5 4,542,384 - Pf6N2E2_3907 0.63 +0.4 4,542,384 - Pf6N2E2_3907 0.63 -0.7 4,542,384 - Pf6N2E2_3907 0.63 -0.8 4,542,384 - Pf6N2E2_3907 0.63 -1.6 4,542,384 - Pf6N2E2_3907 0.63 +1.3 4,542,384 - Pf6N2E2_3907 0.63 -0.0 4,542,522 + +0.8 4,542,522 + +0.5 4,542,522 + -0.5 4,542,523 - +0.3 4,542,523 - -0.8 4,542,524 + -0.2 4,542,524 + +1.2 4,542,525 - -2.7 4,542,549 - +0.9 4,542,764 + +1.6 4,542,765 - -2.6 4,542,984 - Pf6N2E2_3908 0.13 -2.6 4,543,095 - Pf6N2E2_3908 0.20 -1.5 4,543,095 - Pf6N2E2_3908 0.20 -2.5 4,543,098 + Pf6N2E2_3908 0.20 -2.3 4,543,098 + Pf6N2E2_3908 0.20 -1.8 4,543,098 + Pf6N2E2_3908 0.20 -2.7 4,543,098 + Pf6N2E2_3908 0.20 -2.0 4,543,099 - Pf6N2E2_3908 0.20 +0.2 4,543,157 + Pf6N2E2_3908 0.23 -3.0 4,543,187 + Pf6N2E2_3908 0.25 -2.0 4,543,187 + Pf6N2E2_3908 0.25 -2.6 4,543,187 + Pf6N2E2_3908 0.25 -1.8 4,543,188 - Pf6N2E2_3908 0.25 -1.6 4,543,188 - Pf6N2E2_3908 0.25 -2.7 4,543,188 - Pf6N2E2_3908 0.25 -1.3 4,543,188 - Pf6N2E2_3908 0.25 -3.0 4,543,344 - Pf6N2E2_3908 0.35 -3.0 4,543,391 + Pf6N2E2_3908 0.38 -1.6 4,543,392 - Pf6N2E2_3908 0.38 -2.4 4,543,392 - Pf6N2E2_3908 0.38 -1.9 4,543,478 + Pf6N2E2_3908 0.44 -1.5 4,543,478 + Pf6N2E2_3908 0.44 -2.8 4,543,557 - Pf6N2E2_3908 0.48 -1.3 4,543,557 - Pf6N2E2_3908 0.48 -2.3 4,543,666 + Pf6N2E2_3908 0.55 -3.0 4,543,694 + Pf6N2E2_3908 0.57 -3.0 4,543,695 - Pf6N2E2_3908 0.57 -3.5 4,543,695 - Pf6N2E2_3908 0.57 -2.4 4,543,697 + Pf6N2E2_3908 0.57 -2.0 4,543,697 + Pf6N2E2_3908 0.57 -1.2 4,543,727 + Pf6N2E2_3908 0.59 -2.2 4,543,776 - Pf6N2E2_3908 0.62 -2.3 4,543,865 + Pf6N2E2_3908 0.68 -1.8 4,543,866 - Pf6N2E2_3908 0.68 -2.5 4,544,349 - -0.4 4,544,374 + +0.4 4,544,374 + +0.4 4,544,374 + -0.2 4,544,375 - +0.8 4,544,375 - -1.4 4,544,375 - -0.2 4,544,508 - -0.3 4,544,770 - Pf6N2E2_3909 0.14 +0.1 4,545,045 + Pf6N2E2_3909 0.42 -0.1 4,545,046 - Pf6N2E2_3909 0.42 +1.2 4,545,046 - Pf6N2E2_3909 0.42 -0.8 4,545,046 - Pf6N2E2_3909 0.42 +0.1 4,545,046 - Pf6N2E2_3909 0.42 +0.9 4,545,100 + Pf6N2E2_3909 0.48 +1.4 4,545,162 + Pf6N2E2_3909 0.54 +1.6 4,545,162 + Pf6N2E2_3909 0.54 +1.5 4,545,162 + Pf6N2E2_3909 0.54 -1.2 4,545,162 + Pf6N2E2_3909 0.54 -0.0 4,545,162 + Pf6N2E2_3909 0.54 +0.2 4,545,163 - Pf6N2E2_3909 0.54 -0.2 4,545,163 - Pf6N2E2_3909 0.54 -0.7 4,545,163 - Pf6N2E2_3909 0.54 -1.7 4,545,163 - Pf6N2E2_3909 0.54 -0.3 4,545,163 - Pf6N2E2_3909 0.54 -0.0 4,545,163 - Pf6N2E2_3909 0.54 -1.1 4,545,336 + Pf6N2E2_3909 0.72 +1.0 4,545,336 + Pf6N2E2_3909 0.72 -0.3
Or see this region's nucleotide sequence