Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_372 and Pf6N2E2_373 are separated by 288 nucleotides Pf6N2E2_373 and Pf6N2E2_374 are separated by 87 nucleotides Pf6N2E2_374 and Pf6N2E2_375 are separated by 45 nucleotides
Pf6N2E2_372 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 439,581 to 440,507
_372
Pf6N2E2_373 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 440,796 to 442,088
_373
Pf6N2E2_374 - FIG00953249: hypothetical protein, at 442,176 to 442,832
_374
Pf6N2E2_375 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 442,878 to 443,366
_375
Position (kb) 440
441
442
443 Strain fitness (log2 ratio) -2
-1
0
1 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 440.067 kb on + strand, within Pf6N2E2_372 at 440.215 kb on + strand, within Pf6N2E2_372 at 440.274 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.492 kb on + strand at 440.494 kb on + strand at 440.494 kb on + strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.540 kb on + strand at 440.586 kb on - strand at 440.676 kb on + strand at 440.677 kb on - strand at 440.912 kb on - strand at 441.063 kb on + strand, within Pf6N2E2_373 at 441.114 kb on + strand, within Pf6N2E2_373 at 441.115 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.270 kb on + strand, within Pf6N2E2_373 at 441.271 kb on - strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.699 kb on + strand, within Pf6N2E2_373 at 441.700 kb on - strand, within Pf6N2E2_373 at 442.133 kb on + strand at 442.327 kb on + strand, within Pf6N2E2_374 at 442.328 kb on - strand, within Pf6N2E2_374 at 442.341 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.504 kb on - strand, within Pf6N2E2_374 at 442.774 kb on - strand at 442.774 kb on - strand at 442.839 kb on + strand at 442.853 kb on - strand at 442.853 kb on - strand at 443.043 kb on + strand, within Pf6N2E2_375 at 443.043 kb on + strand, within Pf6N2E2_375
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 439,938 + Pf6N2E2_372 0.39 +0.9 439,938 + Pf6N2E2_372 0.39 -0.2 439,938 + Pf6N2E2_372 0.39 -0.1 439,939 - Pf6N2E2_372 0.39 +0.1 439,939 - Pf6N2E2_372 0.39 +0.4 439,939 - Pf6N2E2_372 0.39 -1.1 439,939 - Pf6N2E2_372 0.39 -1.8 440,067 + Pf6N2E2_372 0.52 -0.0 440,215 + Pf6N2E2_372 0.68 +0.3 440,274 - Pf6N2E2_372 0.75 +1.3 440,384 - Pf6N2E2_372 0.87 -0.4 440,384 - Pf6N2E2_372 0.87 -0.7 440,384 - Pf6N2E2_372 0.87 +0.5 440,384 - Pf6N2E2_372 0.87 -0.1 440,492 + -2.5 440,494 + -1.3 440,494 + +1.4 440,495 - -1.7 440,495 - +0.5 440,495 - +0.9 440,540 + -2.2 440,586 - -0.9 440,676 + +1.3 440,677 - +1.3 440,912 - +0.7 441,063 + Pf6N2E2_373 0.21 +1.1 441,114 + Pf6N2E2_373 0.25 -0.0 441,115 - Pf6N2E2_373 0.25 -0.9 441,117 - Pf6N2E2_373 0.25 -0.2 441,117 - Pf6N2E2_373 0.25 +0.3 441,270 + Pf6N2E2_373 0.37 -0.2 441,271 - Pf6N2E2_373 0.37 +0.5 441,330 + Pf6N2E2_373 0.41 +0.1 441,330 + Pf6N2E2_373 0.41 +0.6 441,699 + Pf6N2E2_373 0.70 +1.0 441,700 - Pf6N2E2_373 0.70 +1.4 442,133 + -1.8 442,327 + Pf6N2E2_374 0.23 -0.1 442,328 - Pf6N2E2_374 0.23 +0.3 442,341 + Pf6N2E2_374 0.25 -0.7 442,503 + Pf6N2E2_374 0.50 +0.5 442,503 + Pf6N2E2_374 0.50 +0.4 442,503 + Pf6N2E2_374 0.50 +0.9 442,504 - Pf6N2E2_374 0.50 +0.7 442,774 - +0.3 442,774 - -0.1 442,839 + -2.1 442,853 - -0.0 442,853 - +0.1 443,043 + Pf6N2E2_375 0.34 +0.3 443,043 + Pf6N2E2_375 0.34 +0.1
Or see this region's nucleotide sequence