Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_366 and Pf6N2E2_367 overlap by 4 nucleotides Pf6N2E2_367 and Pf6N2E2_368 are separated by 254 nucleotides
Pf6N2E2_366 - Opine oxidase subunit B, at 432,477 to 433,631
_366
Pf6N2E2_367 - Acetylornithine deacetylase (EC 3.5.1.16), at 433,628 to 434,785
_367
Pf6N2E2_368 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 435,040 to 436,497
_368
Position (kb) 433
434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 432.772 kb on + strand, within Pf6N2E2_366 at 432.895 kb on - strand, within Pf6N2E2_366 at 433.177 kb on - strand, within Pf6N2E2_366 at 433.177 kb on - strand, within Pf6N2E2_366 at 433.204 kb on - strand, within Pf6N2E2_366 at 433.204 kb on - strand, within Pf6N2E2_366 at 433.231 kb on - strand, within Pf6N2E2_366 at 433.231 kb on - strand, within Pf6N2E2_366 at 433.256 kb on + strand, within Pf6N2E2_366 at 433.256 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.311 kb on + strand, within Pf6N2E2_366 at 433.312 kb on - strand, within Pf6N2E2_366 at 433.312 kb on - strand, within Pf6N2E2_366 at 433.416 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.470 kb on + strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.471 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.474 kb on - strand, within Pf6N2E2_366 at 433.642 kb on - strand at 433.917 kb on - strand, within Pf6N2E2_367 at 433.917 kb on - strand, within Pf6N2E2_367 at 434.318 kb on + strand, within Pf6N2E2_367 at 434.528 kb on + strand, within Pf6N2E2_367 at 434.783 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 435.007 kb on + strand at 435.018 kb on + strand at 435.088 kb on + strand at 435.089 kb on - strand at 435.174 kb on - strand at 435.427 kb on + strand, within Pf6N2E2_368
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 432,772 + Pf6N2E2_366 0.26 +0.9 432,895 - Pf6N2E2_366 0.36 -0.5 433,177 - Pf6N2E2_366 0.61 -0.5 433,177 - Pf6N2E2_366 0.61 +0.7 433,204 - Pf6N2E2_366 0.63 -0.5 433,204 - Pf6N2E2_366 0.63 +0.5 433,231 - Pf6N2E2_366 0.65 -0.6 433,231 - Pf6N2E2_366 0.65 +0.2 433,256 + Pf6N2E2_366 0.67 -1.9 433,256 + Pf6N2E2_366 0.67 -0.4 433,311 + Pf6N2E2_366 0.72 +1.1 433,311 + Pf6N2E2_366 0.72 -1.4 433,311 + Pf6N2E2_366 0.72 +0.7 433,312 - Pf6N2E2_366 0.72 +1.2 433,312 - Pf6N2E2_366 0.72 +0.6 433,416 + Pf6N2E2_366 0.81 +0.9 433,470 + Pf6N2E2_366 0.86 +0.8 433,470 + Pf6N2E2_366 0.86 +0.5 433,470 + Pf6N2E2_366 0.86 +2.1 433,470 + Pf6N2E2_366 0.86 +0.4 433,470 + Pf6N2E2_366 0.86 -0.4 433,470 + Pf6N2E2_366 0.86 -1.9 433,471 - Pf6N2E2_366 0.86 -0.8 433,471 - Pf6N2E2_366 0.86 +1.2 433,471 - Pf6N2E2_366 0.86 -0.4 433,471 - Pf6N2E2_366 0.86 -0.0 433,471 - Pf6N2E2_366 0.86 -0.9 433,471 - Pf6N2E2_366 0.86 -1.5 433,474 - Pf6N2E2_366 0.86 +0.2 433,474 - Pf6N2E2_366 0.86 -1.7 433,474 - Pf6N2E2_366 0.86 +1.0 433,474 - Pf6N2E2_366 0.86 +0.1 433,642 - -0.4 433,917 - Pf6N2E2_367 0.25 -0.1 433,917 - Pf6N2E2_367 0.25 +0.0 434,318 + Pf6N2E2_367 0.60 -0.7 434,528 + Pf6N2E2_367 0.78 -0.3 434,783 + +0.6 434,792 + +0.9 434,792 + -0.5 434,792 + +0.4 434,792 + -0.9 434,792 + -1.6 434,792 + +0.5 434,793 - +0.1 434,793 - -2.7 434,793 - +1.1 434,793 - -0.0 434,793 - +0.7 434,800 + -1.4 434,800 + +0.3 434,800 + +0.0 434,801 - -0.4 434,801 - +0.5 434,801 - +0.4 434,893 + -0.1 434,893 + -2.8 434,893 + +0.6 434,894 - -0.5 434,894 - -0.1 434,894 - +0.1 434,894 - -0.7 434,894 - +1.3 434,894 - -0.5 435,007 + +0.2 435,018 + +0.7 435,088 + +0.5 435,089 - +0.6 435,174 - -0.1 435,427 + Pf6N2E2_368 0.27 -0.5
Or see this region's nucleotide sequence