Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3650 and Pf6N2E2_3651 overlap by 4 nucleotides Pf6N2E2_3651 and Pf6N2E2_3652 are separated by 34 nucleotides Pf6N2E2_3652 and Pf6N2E2_3653 are separated by 147 nucleotides
Pf6N2E2_3650 - Oxidoreductase, FAD-binding, at 4,250,313 to 4,251,488
_3650
Pf6N2E2_3651 - OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY, at 4,251,485 to 4,252,297
_3651
Pf6N2E2_3652 - Cation transport ATPase, at 4,252,332 to 4,253,297
_3652
Pf6N2E2_3653 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 4,253,445 to 4,254,866
_3653
Position (kb)
4252
4253
4254 Strain fitness (log2 ratio)
-1
0
1
2 at 4252.272 kb on + strand at 4252.340 kb on + strand at 4252.416 kb on + strand at 4252.416 kb on + strand at 4252.416 kb on + strand at 4252.417 kb on - strand at 4252.417 kb on - strand at 4252.564 kb on - strand, within Pf6N2E2_3652 at 4252.564 kb on - strand, within Pf6N2E2_3652 at 4252.630 kb on - strand, within Pf6N2E2_3652 at 4252.908 kb on + strand, within Pf6N2E2_3652 at 4252.909 kb on - strand, within Pf6N2E2_3652 at 4252.909 kb on - strand, within Pf6N2E2_3652 at 4253.026 kb on - strand, within Pf6N2E2_3652 at 4253.026 kb on - strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.067 kb on + strand, within Pf6N2E2_3652 at 4253.068 kb on - strand, within Pf6N2E2_3652 at 4253.068 kb on - strand, within Pf6N2E2_3652 at 4253.068 kb on - strand, within Pf6N2E2_3652 at 4253.089 kb on + strand, within Pf6N2E2_3652 at 4253.089 kb on + strand, within Pf6N2E2_3652 at 4253.089 kb on + strand, within Pf6N2E2_3652 at 4253.090 kb on - strand, within Pf6N2E2_3652 at 4253.090 kb on - strand, within Pf6N2E2_3652 at 4253.090 kb on - strand, within Pf6N2E2_3652 at 4253.116 kb on - strand, within Pf6N2E2_3652 at 4253.295 kb on + strand at 4253.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,252,272 + -1.0 4,252,340 + +1.5 4,252,416 + +0.0 4,252,416 + +0.2 4,252,416 + -0.1 4,252,417 - +0.3 4,252,417 - +1.4 4,252,564 - Pf6N2E2_3652 0.24 -0.3 4,252,564 - Pf6N2E2_3652 0.24 +0.8 4,252,630 - Pf6N2E2_3652 0.31 -0.1 4,252,908 + Pf6N2E2_3652 0.60 +1.7 4,252,909 - Pf6N2E2_3652 0.60 +0.9 4,252,909 - Pf6N2E2_3652 0.60 +0.8 4,253,026 - Pf6N2E2_3652 0.72 -0.3 4,253,026 - Pf6N2E2_3652 0.72 +1.4 4,253,067 + Pf6N2E2_3652 0.76 +1.3 4,253,067 + Pf6N2E2_3652 0.76 +0.6 4,253,067 + Pf6N2E2_3652 0.76 +1.3 4,253,067 + Pf6N2E2_3652 0.76 +1.0 4,253,067 + Pf6N2E2_3652 0.76 +1.3 4,253,067 + Pf6N2E2_3652 0.76 +0.4 4,253,068 - Pf6N2E2_3652 0.76 +0.5 4,253,068 - Pf6N2E2_3652 0.76 +1.3 4,253,068 - Pf6N2E2_3652 0.76 +1.7 4,253,089 + Pf6N2E2_3652 0.78 +1.5 4,253,089 + Pf6N2E2_3652 0.78 +1.9 4,253,089 + Pf6N2E2_3652 0.78 +0.9 4,253,090 - Pf6N2E2_3652 0.78 +1.1 4,253,090 - Pf6N2E2_3652 0.78 -0.8 4,253,090 - Pf6N2E2_3652 0.78 +1.5 4,253,116 - Pf6N2E2_3652 0.81 +1.0 4,253,295 + +1.0 4,253,437 - +1.1
Or see this region's nucleotide sequence